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Investigation of Antioxidant Activities and Hypoglycemic Effect of Black and White Myrtle Fruits

Year 2021, Volume: 14 Issue: 1, 314 - 321, 31.03.2021

Sevim Çiftçi Yegin , Aytaç Güder

https://doi.org/10.18185/erzifbed.804421
Cited By: 1

Abstract

Fruits, are in black or white colour Mrytus communis, is known as locally name murt, hambales, mersin. It is an aromatic and medicinal plant that grows naturally in maquis form in regions with Mediterranean climate. We aimed to investigate the hypoglycemic effect and antioxidant activity of Myrtus communis L. in this research. White-black myrtle were collected from Hassa-Hatay and Tarsus-Mersin, respectively in October. Fruits were separated from the leaves and dried in the oven at 40ºC. Fruit extracts were obtained in absolute ethanol (1:15) by using magnetic stirrer at the room temperature for one day.
In this study, radical scaving activity-(DPPH), the hydrogen peroxide scavenging activity-(HPSA), metal chelating activity-(MCA), ferric reducing antioxidant power activity-(FRAP), total phenol-flavonoid contents and α-amylase, α-glycosidase inhibition were examined to determine characteristics of samples. DPPH and HPSA values were expressed as µg/ml in terms of SC50. FRAP and MCA features of extract were given as % activity. The hypoglycemic effect was investigated with -amylase--glucosidase inhibition assays. The DPPH-HPSA-FRAP-MCA of white-black myrtle samples were found 56.75-63.52 µg/ml, 193.95-207.73 µg/ml, 81.68-90.87%, and 20.88-27.71%, respectively. When the antioxidant results compare with the used standards such as Buthylated-Hydroxy-Anisole, Rutin, Trolox, white-black myrtle samples have effective antioxidant activity. Samples have shown hypoglycemic effect.

Keywords

Antioxidant activities Hypoglycemic effect Mrytus communis L.

References

  • Alexander, R.J. (1992). “Production and Applications”, World Wide Technology. New York, VHC Publishers.
  • Asgarpanah, J., Ariamanesh, A. 2015. “Phytochemistry and pharmacological properties of Myrtus communis L.”, Indian Journal of Traditional Knowledge, 1(1), 82-87.
  • Asif, H.M., Akram, M., Uddin, S., UlHasan, Z., Sami, A., Iqbal, A., Tauseef, A., Bari, A. 2011. “Myrtus communis L. (Pharmacological activity)”, Journal of Medicinal Plants Research, 5(26), 6257-6259.
  • Baharvand-Ahmadi, B., Bahmani, M., Naghdi, N., Saki, K., Baharvand-Ahmadi, S., Rafieian-Kopaei, M. 2015. “Review on phytochemistry, therapeutic and pharmacological effects of myrtus (Myrtus communis)”, Scholars Research Library Der Pharmacia Lettre, 7 (11), 160-165.
  • Baz, H. 2014. “Streptozotocı̇nle ı̇ndüklenen dı̇yabetlı̇ ratlar üzerı̇nde Myrtus communis L. yaprağı su ekstresı̇ etkı̇lerı̇nı̇n araştırılması”, Yüksek Lisans Tezi, Atatürk Üniversitesi Sağlık Bilimleri Enstitüsü, Erzurum,69-70.
  • Benkhayal, F.A., Musbah, E.G., Ramesh, S., Dhayabaran, D.2009. “Biochemical studies on the effect of phenolic compounds extracted from Myrtus communis in diabetic rats”, Tamil Nadu Journal of Veterinary Animal and Sciences, 5 (3), 87-93.
  • Bernfeld, P. (1955). “Methods in Enzymology”, New York, Academic Press.
  • Blois, M.S. 1958. “Antioxidant determinations by the use of a stable free radical”, Nature, 26, 1199-1200.
  • Chang, C.C., Yang, M.H., Wen, H.M., Chern, J.C. 2002. “Estimation of total flavonoid content in propolis by two complementary colorimetric methods”, Journal of Food and Drug Analysis, 10, 178-182.
  • Chryssavgi, G., Vassiliki, P., Athanasios, M., Kibouris, T., Michael, K. 2008. “Essential oil composition of Pistacia lentiscus L. and Myrtus communis L.: Evaluation of antioxidant capacity of methanolic extracts”, Food Chemistry, 107, 1120-1130.
  • Davies, G.J., Henrissat, B. 1995. “Structures and mechanisms of glycosyl hydrolases”, Structure, 3 (9), 853-859.
  • Demir, G.M., Gulaboglu, M., Aggul, A.G., Kilic Baygutalp, N., Canayakin, D., Halici, Z., Suleyman, H. 2011. “Antioxidant and antidiabetic activity of aqueous extract of Myrtus Communis L. berries on streptozotocin-induced diabetic rats”, IOSR Journal of Pharmacy and Biological Sciences, 11(5), 11-16.
  • Derwich, E., Benziane, Z., Chabir, R., Taouil, R. 2011. “Characterization of volatiles and evaluation of antioxidant activity of the flower essential oils of Myrtus communis L. from Morocco”, Name The Journal, 3(3), 17-23.
  • Dinis, T.C.P., Madeira, V.M.C., Almeida, L.M. 1994. “Action of phenolic derivates (acetoaminophen, salycilate, and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers”, Archives Biochemistry and Biophysics, 315, 161-169.
  • Durmuş, R.N., Şahin, E., Bireller, S. 2016. “Gestasyonel dı̇yabette hı̇poglı̇semı̇k etkı̇lı̇ bı̇tkı̇lerı̇n kullanımı”, Deneysel Tıp Araştırma Enstitüsü Dergisi, 6(11), 3-16.
  • Ferchichi, H., Salouage, I., Bacha, S., Ben Said, D., Gaies, E., Boussaid, M., Lakhal, M., Klouz A. 2011. “Effect of Myrtus communis L. extracts on attenuation of liver normothermic ischemia-reperfusion injury”, Journal of Transplantation Technologies  Research, doi:10.4172/2161-0991.S3-001.
  • Güder, A. 2016. “Influence of total anthocyanins from bitter melon (Momordica charantia Linn.) as antidiabetic and radical scavenging agents”, Iran Journal of Pharmaceutical Research, 15 (1), 301-309.
  • Johari, H., Nozari, M., Moghtari, M., Zamani, Z., Yazdani, M. 2014. “The effect of myrtus communis extract on liver enzymes and blood biochemical factors in diabetic adult male rats”, Zahedan Journal of Research in Medical Sciences, 16, 12-17.
  • Karaman, Ö., Elgin Cebe, G. 2016. “Dı̇yabet ve Türkı̇ye’de antı̇dı̇yabetı̇k olarak kullanılan bı̇tkı̇ler”, Journal of Faculty of Pharmacy of Ankara University, 40(3), 47-61.
  • Lebovit, H. 1998. “-Glucosidase inhibitors as agents in the treatment of diabetes”, Diabetes Reviewer, 6, 132-145.
  • McCue, P., Kwon, Y.I., Shetty, K. 2005. “Anti-amylase, anti-glucosidase and anti-angiotensin I-converting enzyme potential of selected foods”, Journal of Food Biochemistry, 29, 278-294.
  • Mimica-Dukic, N., Bugarin, D., Grbovic, S., Mitic-Culafic, D., Vukovic-Gacic, B., Orcic, D., Jovin, E., Couladis, M. 2010. “Essential oil of Myrtus communis L. as a potential antioxidant and antimutagenic agents”, Molecules, 15, 2759-2770.
  • Montoro, P., Tuberoso, C.I.G., Piacente, S., Perrone, A., De Feo, V., Cabras, P., Pizza, C. 2006. “Stability and antioxidant activity of polyphenols in extracts of Myrtus communis L. berries used for the preparation of myrtle liqueur”, Journal of Pharmaceutical and Biomedical Analysis, 41, 1614-1619.
  • Naczk, M., Shahidi, F. 2004. “Extraction and analysis of phenolics in food”, Journal of Chromatography A, 1054 (1-2), 95-111.
  • Önal, S., Timur, S., Okutucu, B., Zihnioğlu, F. 2005. “Inhibition of ‐glucosidase by aqueous extracts of some potent antidiabetic medicinal herbs”, Preparative Biochemistry and Biotechnology, 35(1), 29-36.
  • Oyaizu, M. 1986. “Studies on products of browning reactions: Antioxidative activities of product of browning reaction prepared from glucosamine”, Japan Journal of Nutrition, 44, 307-315.
  • Panjeshahin, M.R., Azadbakht, M., Akbari, N. 2016. “Antidiabetic activity of different extracts of Myrtus communis in streptozotocin induced diabetic rats”, Romanian Journal of Diabetes Nutrition and Metabolic Diseases, 23(2), 183-190.
  • Ruch, R.J., Cheng, S., Klaunig, J.E. 1989. “Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea”, Carcinogenesis, 10, 1003-1008.
  • Sepici Dincel, A., Gürbüz, I., Çevik, C., Yesilada, E. 2004. “Hypoglycaemic effects of myrtle oil in normal and alloxan-diabetic rabbits”, Journal of Ethnopharmacology, 93, 311-318.
  • Sepici-Dincel, A., Açıkgöz, Ş., Çevik, C., Sengelen, M., Yeşilada, E. 2007. “Effects of in vivo antioxidant enzyme activities of myrtle oil in normoglycaemic and alloxan diabetic rabbits”, Journal of Ethnopharmacology, 110, 498-503.
  • Slinkard, K., Singleton, V.L. 1977. “Total phenol analyses: automation and comparison with manual methods”, American Journal of Enology and Viticulture, 28, 49-55.
  • Tsao, R., Yang, R. 2003. “Optimization of a new mobile phase to know the complex and real polyphenolic composition: towards a total phenolic index using high-performance liquid chromatography”, Journal of Chromatography, 1018, 29-40.
  • Wang, H., Nair, M.G., Strasburg, G.M., Chang, Y.C., Booren, A.M., Gray, J.I., Dewitt, D.L. 1999. “Antioxidant and antiinflammatory activities of anthocyanins and their aglycon, cyanidin, from tart cherries”, Journal of Natural Product, 62(2), 294-296.

Year 2021, Volume: 14 Issue: 1, 314 - 321, 31.03.2021

Sevim Çiftçi Yegin , Aytaç Güder

https://doi.org/10.18185/erzifbed.804421
Cited By: 1

Abstract

Latincesi Myrtus communis L. olan halk arasında murt, hambeles, mersin diye adlandırılan meyve siyah veya beyaz renkte olmaktadır. Tıbbi ve aromatik bir bitki olan mersin, akdeniz ikliminin yaygın olduğu bölgelerde doğal olarak yetişir. Bu çalışmada, Myrtus communis L. nin antioksidan aktivitesini ve hipoglisemik etkisini belirlemek amaçlanmıştır.
Beyaz ve siyah mersin örnekleri sırayla Hassa-Hatay ve Tarsus-Mersin den hasat dönemi olan Ekim ayında toplanmıştır. Meyveler yapraklarından ayrılarak etüvde 40º C de kurutuldu. Kuru örnekler toz haline getirilerek, absolute etanol (1:15) ile 1 gün boyunca manyetik karıştırıcıda karıştırılıp, süzgeç kağıdı ile süzülerek örneklerin özütü elde edildi.
Bu çalışmada Radikal giderme aktivitesi (DPPH), Hidrojen peroksit giderme aktivitesi (HPSA), Demir indirgeme kapasitesi (FRAP) ve Metal şelat oluşturma aktivitesi (MŞA), Total feneol-flavonoid içerikleri ile hipoglisemik etki için α-amilaz, α-glikozidaz inhibisyonu araştırıldı. DPPH ve HPSA değerleri SC50 ile FRAP ve MŞA ise % olarak ifade edildi. Hipoglisemik etki ise α-amilaz, α-glikozidaz inhibisyonu ölçümü ile belirlendi. Beyaz ve siyah murt örneklerinde DPPH, HPSA, FRAP, MŞA değerleri sırasıyla 56.75-63.52 µg/ml, 193.95-207.73 µg/ml, 81.68-90.87%, 20.88-27.71% bulunmuştur. Antioksidan sonuçlar Bütilat Hidroksi Anisol (BHA), Rutin (RUT) ve Trolox (TRO) gibi standartlarla karşılaştırıldığında beyaz ve siyah murt örnekleri etkili bir antioksidan aktivite göstermiştir. Örnekler aynı zamanda etkili bir hipoglisemik etki göstermiştir.

Keywords

antioksidan aktivite hipoglisemik etki myrtus communis L

References

  • Alexander, R.J. (1992). “Production and Applications”, World Wide Technology. New York, VHC Publishers.
  • Asgarpanah, J., Ariamanesh, A. 2015. “Phytochemistry and pharmacological properties of Myrtus communis L.”, Indian Journal of Traditional Knowledge, 1(1), 82-87.
  • Asif, H.M., Akram, M., Uddin, S., UlHasan, Z., Sami, A., Iqbal, A., Tauseef, A., Bari, A. 2011. “Myrtus communis L. (Pharmacological activity)”, Journal of Medicinal Plants Research, 5(26), 6257-6259.
  • Baharvand-Ahmadi, B., Bahmani, M., Naghdi, N., Saki, K., Baharvand-Ahmadi, S., Rafieian-Kopaei, M. 2015. “Review on phytochemistry, therapeutic and pharmacological effects of myrtus (Myrtus communis)”, Scholars Research Library Der Pharmacia Lettre, 7 (11), 160-165.
  • Baz, H. 2014. “Streptozotocı̇nle ı̇ndüklenen dı̇yabetlı̇ ratlar üzerı̇nde Myrtus communis L. yaprağı su ekstresı̇ etkı̇lerı̇nı̇n araştırılması”, Yüksek Lisans Tezi, Atatürk Üniversitesi Sağlık Bilimleri Enstitüsü, Erzurum,69-70.
  • Benkhayal, F.A., Musbah, E.G., Ramesh, S., Dhayabaran, D.2009. “Biochemical studies on the effect of phenolic compounds extracted from Myrtus communis in diabetic rats”, Tamil Nadu Journal of Veterinary Animal and Sciences, 5 (3), 87-93.
  • Bernfeld, P. (1955). “Methods in Enzymology”, New York, Academic Press.
  • Blois, M.S. 1958. “Antioxidant determinations by the use of a stable free radical”, Nature, 26, 1199-1200.
  • Chang, C.C., Yang, M.H., Wen, H.M., Chern, J.C. 2002. “Estimation of total flavonoid content in propolis by two complementary colorimetric methods”, Journal of Food and Drug Analysis, 10, 178-182.
  • Chryssavgi, G., Vassiliki, P., Athanasios, M., Kibouris, T., Michael, K. 2008. “Essential oil composition of Pistacia lentiscus L. and Myrtus communis L.: Evaluation of antioxidant capacity of methanolic extracts”, Food Chemistry, 107, 1120-1130.
  • Davies, G.J., Henrissat, B. 1995. “Structures and mechanisms of glycosyl hydrolases”, Structure, 3 (9), 853-859.
  • Demir, G.M., Gulaboglu, M., Aggul, A.G., Kilic Baygutalp, N., Canayakin, D., Halici, Z., Suleyman, H. 2011. “Antioxidant and antidiabetic activity of aqueous extract of Myrtus Communis L. berries on streptozotocin-induced diabetic rats”, IOSR Journal of Pharmacy and Biological Sciences, 11(5), 11-16.
  • Derwich, E., Benziane, Z., Chabir, R., Taouil, R. 2011. “Characterization of volatiles and evaluation of antioxidant activity of the flower essential oils of Myrtus communis L. from Morocco”, Name The Journal, 3(3), 17-23.
  • Dinis, T.C.P., Madeira, V.M.C., Almeida, L.M. 1994. “Action of phenolic derivates (acetoaminophen, salycilate, and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers”, Archives Biochemistry and Biophysics, 315, 161-169.
  • Durmuş, R.N., Şahin, E., Bireller, S. 2016. “Gestasyonel dı̇yabette hı̇poglı̇semı̇k etkı̇lı̇ bı̇tkı̇lerı̇n kullanımı”, Deneysel Tıp Araştırma Enstitüsü Dergisi, 6(11), 3-16.
  • Ferchichi, H., Salouage, I., Bacha, S., Ben Said, D., Gaies, E., Boussaid, M., Lakhal, M., Klouz A. 2011. “Effect of Myrtus communis L. extracts on attenuation of liver normothermic ischemia-reperfusion injury”, Journal of Transplantation Technologies  Research, doi:10.4172/2161-0991.S3-001.
  • Güder, A. 2016. “Influence of total anthocyanins from bitter melon (Momordica charantia Linn.) as antidiabetic and radical scavenging agents”, Iran Journal of Pharmaceutical Research, 15 (1), 301-309.
  • Johari, H., Nozari, M., Moghtari, M., Zamani, Z., Yazdani, M. 2014. “The effect of myrtus communis extract on liver enzymes and blood biochemical factors in diabetic adult male rats”, Zahedan Journal of Research in Medical Sciences, 16, 12-17.
  • Karaman, Ö., Elgin Cebe, G. 2016. “Dı̇yabet ve Türkı̇ye’de antı̇dı̇yabetı̇k olarak kullanılan bı̇tkı̇ler”, Journal of Faculty of Pharmacy of Ankara University, 40(3), 47-61.
  • Lebovit, H. 1998. “-Glucosidase inhibitors as agents in the treatment of diabetes”, Diabetes Reviewer, 6, 132-145.
  • McCue, P., Kwon, Y.I., Shetty, K. 2005. “Anti-amylase, anti-glucosidase and anti-angiotensin I-converting enzyme potential of selected foods”, Journal of Food Biochemistry, 29, 278-294.
  • Mimica-Dukic, N., Bugarin, D., Grbovic, S., Mitic-Culafic, D., Vukovic-Gacic, B., Orcic, D., Jovin, E., Couladis, M. 2010. “Essential oil of Myrtus communis L. as a potential antioxidant and antimutagenic agents”, Molecules, 15, 2759-2770.
  • Montoro, P., Tuberoso, C.I.G., Piacente, S., Perrone, A., De Feo, V., Cabras, P., Pizza, C. 2006. “Stability and antioxidant activity of polyphenols in extracts of Myrtus communis L. berries used for the preparation of myrtle liqueur”, Journal of Pharmaceutical and Biomedical Analysis, 41, 1614-1619.
  • Naczk, M., Shahidi, F. 2004. “Extraction and analysis of phenolics in food”, Journal of Chromatography A, 1054 (1-2), 95-111.
  • Önal, S., Timur, S., Okutucu, B., Zihnioğlu, F. 2005. “Inhibition of ‐glucosidase by aqueous extracts of some potent antidiabetic medicinal herbs”, Preparative Biochemistry and Biotechnology, 35(1), 29-36.
  • Oyaizu, M. 1986. “Studies on products of browning reactions: Antioxidative activities of product of browning reaction prepared from glucosamine”, Japan Journal of Nutrition, 44, 307-315.
  • Panjeshahin, M.R., Azadbakht, M., Akbari, N. 2016. “Antidiabetic activity of different extracts of Myrtus communis in streptozotocin induced diabetic rats”, Romanian Journal of Diabetes Nutrition and Metabolic Diseases, 23(2), 183-190.
  • Ruch, R.J., Cheng, S., Klaunig, J.E. 1989. “Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea”, Carcinogenesis, 10, 1003-1008.
  • Sepici Dincel, A., Gürbüz, I., Çevik, C., Yesilada, E. 2004. “Hypoglycaemic effects of myrtle oil in normal and alloxan-diabetic rabbits”, Journal of Ethnopharmacology, 93, 311-318.
  • Sepici-Dincel, A., Açıkgöz, Ş., Çevik, C., Sengelen, M., Yeşilada, E. 2007. “Effects of in vivo antioxidant enzyme activities of myrtle oil in normoglycaemic and alloxan diabetic rabbits”, Journal of Ethnopharmacology, 110, 498-503.
  • Slinkard, K., Singleton, V.L. 1977. “Total phenol analyses: automation and comparison with manual methods”, American Journal of Enology and Viticulture, 28, 49-55.
  • Tsao, R., Yang, R. 2003. “Optimization of a new mobile phase to know the complex and real polyphenolic composition: towards a total phenolic index using high-performance liquid chromatography”, Journal of Chromatography, 1018, 29-40.
  • Wang, H., Nair, M.G., Strasburg, G.M., Chang, Y.C., Booren, A.M., Gray, J.I., Dewitt, D.L. 1999. “Antioxidant and antiinflammatory activities of anthocyanins and their aglycon, cyanidin, from tart cherries”, Journal of Natural Product, 62(2), 294-296.
There are 33 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Makaleler
Authors

Sevim Çiftçi Yegin 0000-0003-3950-4399

Aytaç Güder 0000-0002-1190-8749

Publication Date March 31, 2021
Published in Issue Year 2021 Volume: 14 Issue: 1

Cite

APA Çiftçi Yegin, S., & Güder, A. (2021). Investigation of Antioxidant Activities and Hypoglycemic Effect of Black and White Myrtle Fruits. Erzincan University Journal of Science and Technology, 14(1), 314-321. https://doi.org/10.18185/erzifbed.804421

Cited By

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Sustainability
https://doi.org/10.3390/su142013210