Analysis of Atenolol in Rabbit Plasma by HPLC Method

Year 2023, Volume: 16 Issue: 1, 1 - 10, 31.03.2023

Bilal Yilmaz

https://doi.org/10.18185/erzifbed.1083703

Abstract

The goal of this research is to develop a high-performance liquid chromatography method for analyzing atenolol levels in rabbit plasma and apply this method to the pharmacokinetic study. The liquid-liquid extraction technique was used to prepare blood samples from rabbits. Separation of atenolol was achieved on an Ace C18 column. The method’s calibration curve was plotted between 5 and 250 ng/mL. The accuracy results were better than 2.97% and the precision results were less than 6.30% in rabbit plasma for atenolol. The method had recovery values >95.8% for all samples in rabbit plasma. In addition, the validated method was used to study atenolol pharmacokinetics in rabbits. The maximum atenolol plasma concentration is 240.1 ± 33.41 ng/mL. The duration to attain the greatest atenolol concentration and the area under the curve from (AUC0-12 h) were 3.0 ± 0.64 h and 1184.1 ± 235.13 ng/mL h, respectively.

Keywords

Atenolol, HPLC, extraction, pharmacokinetic, rabbit

Thanks

The author would like to thank Abdi Ibrahim Pharmaceutical Industry for atenolol standard. The author also wishes to thank Vedat Akba (Criminal Police Laboratory, 25060, Erzurum, Turkey) for their help in GC-MS analyses.

References

• Al-Ghannam, S.M. 2006. ‘‘A simple spectrophotometric method for the determination of β- blockers in dosage forms’’, Journal of Pharmaceutical and Biomedical Analysis, 40, 151-156.
• Antakli, S., Nejem, L., Joumaa, M.A. 2020. ‘’Determination of atenolol in tablet formulation by analytical spectrophotometry’’. Research Journal of Pharmacy and Technology, 13(2), 609-614.
• Arias, R., Jimenez, R.M., Alonso, R.M., Telez, M., Arrieta, I., Flores, P., Ortiz-Lastra, E. 2001. ‘‘Determination of the β-blocker atenolol in plasma by capillary zone electrophoresis’’, Journal of Chromatography A , 916, 297-304.
• Chatterjee, D.J., Li, W.Y., Hurst, A.K., Koda, R.T. 1995. ‘‘High-performance liquid chromatographic method for determination of atenolol from human plasma and urine: simultaneous fluorescence and ultraviolet detection’’, Journal of Liquid Chromatogr, 18, 791-806.
• Chiu, F.C., Zhang, J.N., Li, R.C., Raymond, K. 1997. ‘‘Efficient assay for the determination of atenolol in human plasma and urine by high-performance liquid chromatography with fluorescence detection’’, Journal of Chromatography B, 691, 473-477.
• El-Alfy, W., Ismaiel, O.A., El-Mammli, M.Y., Shalaby, A. 2019. ‘’Determination of atenolol and trimetazidine in pharmaceutical tablets and human urine using a high-performance liquid chromatography-photo diode array detection method’’, International Journal of Analytical Chemistry. Article ID 9625849.
• Elkady, E.F., Fouad, M.A., Faquih, A.A. 2020. ‘’A versatile stability-indicating liquid chromatographic method for the simultaneous determination of atenolol, hydrochlorothiazide and chlorthalidone’’, Current Pharmaceutical Analysis, 16(8):1037-1051.
• Giachetti, C., Tencoti, A., Canali, S., Zanolo, G. 1997. ‘‘Simultaneous determination of atenolol and chlorthalidone in plasma by high-performance liquid chromatography Application to pharmacokinetic studies in man’’, Journal of Chromatography B, 698, 187-194.
• Guidance for Industry, Bioanalytical Method Validation, US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (CDER), Rockville, MD, 2001.
• Iha, M.H., Martinez, A.S., Bonato, P.S. 2002. ‘‘Enantioselective analysis of atenolol in biologic fluids: comparison of liquid-liquid and solid-phase extraction methods’’, Journal of Chromatography B, 767, 1-9.
• Li, W., Li, Y., Francisco, D.T., Naidong, W. 2005. ‘‘Hydrophilic interaction liquid chromatographic tandem mass spectrometric determination of atenolol in human plasma’’, Biomedical Chromatography, 19, 385-393.
• Madhusudhan, P., Reddy, K.V., Singh, S. 2018. ‘’A novel isocratic RP-HPLC method development and validation for simultaneous determination of atenolol and nifedipine in tablet dosage form’’, Universal Review 7(9): 412-421.
• Miller, R.B. 1991. ‘‘A validated high-performance liquid chromatographic method for the determination of atenolol in whole blood’’, Journal of Pharmaceutical and Biomedical Analysis, 9, 849-853.
• Mohammad, M.Y., Abdullah, M.S., Sabir, S.S. 2019. ‘’Simultaneous determination of atenolol and amlodipine using second derivative spectroscopy’’, Polytechnic Journal, 9(2), 25-29.
• Morris, R.G., Saccoia, N.C., Sallustio, B.C., Zacest, R. 1991. ‘‘Improved high-performance liquid chromatography assay for atenolol in plasma and urine using fluorescence detection’’, Therapeutic Drug Monitoring, 13, 345-349.
• Pires de Abreu, L.R., Calafatti de Castro, S.A., Pedrazzoli Jr, J. 2003. ‘‘Atenolol quantification in human plasma by high-performance liquid chromatography: application to bioequivalance study’’, AAPS PharmSci, 5, 1-7.
• Saleem, B.A. 2019. ‘’Spectrophotometric determination of atenolol using ındigo carmine dye’’, Kirkuk University Journal for Scientific Studies, 14(2), 15-39.
• Sethi, P.D. 2001. ‘‘High Performance Liqid Chromatography-Quantitative Analysis of Pharmaceutical Formulations’’, CBS Publishers & Distributors, Mumbai, 3-212.
• Tabrizi, A.B., Yousefzadeh, F. 2019. ‘’Spectrofluorimetric determination of atenolol and carvedilol in pharmaceutical preparations after optimization of parameters using response surface methodology’’, Pharmaceutical Sciences, 25(3), 262-267.
• Vaikosen, E.N., Bioghele, J., Worlu, R.C., Ebeshi, B.U. 2020. ‘’Spectroscopic determination of two beta-blockers–atenolol and propanolol by oxidative derivatization using potassium permanganate in alkaline medium’’, Reviews in Analytical Chemistry, 39(1): 56-64.
• Yilmaz, B., Arslan, S. 2009. ‘‘GC-MS determination of atenolol plasma concentration after derivatization with N-methyl-N-(trimethylsilyl)trifluoroacetamide’’, Chromatographia, 70(9-10), 1399-1404.