TANACETUM ZAHLBRUCKNERI (NÁB.) GRIERSON’ NUN SEKONDER METABOLİTLERİ ve ANTİOKSİDAN ETKİLERİ

Year 2017, Volume: 26 Issue: 1, 71 - 77, 01.03.2017

Perihan Gürbüz , Duygu Eroğlu Şengül Dilem Doğan Gökçe Şeker Karatoprak Mehmet Yavuz Paksoy Müberra Koşar

Abstract

Bu çalışma kapsamında Asteraceae familyasına dahil
endemik bir tür olan Tanacetum zahlbruckneri (Náb.)
Grierson bitkisinin toprak üstü kısımlarından hazırlanan
metanol ekstresinin DPPH●
, ABTS●+ radikallerini
süpürücü aktivitesi ve β-karoten-linoleik asitle oksidasyon yöntemi ile antioksidan aktiviteleri, Folin– Ciocalteu
yöntemiyle toplam fenol, flavonit ve flavonol içeriği
incelenmiştir. Metanol ekstresinden sıvı-sıvı partisyon
yöntemiyle n-hekzan, dietil eter, diklorometan ve n- butanol ekstreleri hazırlanmış, n-butanol ekstresinden
çeşitli kromatografik yöntemler kullanılarak, klorojenik
asit (1), rutin (2) ve konduritol F (3) izole edilmiştir.
Elde edilen bileşiklerin yapıları spektroskopik yöntemler (IR, 1H-NMR, 13C-NMR, 2D-NMR, IR, MS) kullanılarak
aydınlatılmıştır. Bu çalışma ile Konduritol F Tanacetum
türlerinden ilk defa izole edilmiştir. Klorojenik asit ve
rutinin bitkinin metanol ekstresindeki kantitatif tayini
Yüksek Basınçlı Sıvı Kromatografisi ile gerçekleştirilmiştir. İzole edilen bileşiklerin antioksidan aktiviteleri ölçülmüş ve klorojenik asitin aktivitesi, rutin ve
metanol ekstresinden daha yüksek bulunmuştur.

Keywords

Tanacetum zahlbruckneri, Klorojenik asit, Rutin, Konduritol F, HPLC

Secondary Metabolites and Antioxidant Activities of Tanacetum Zahlbruckneri(Náb.)Grierson

Abstract

In the current study, antioxidant activity of methanol
extract prepared from Tanacetum zahlbruckneri
(Asteraceae) was studied with DPPH●, ABTS+ radical
scavenging and beta-carotene-linoleic acid cooxidation
assays. Total phenol, flavonoid and flavonol contents of
extracts were determined with Folin- Ciocalteu method. n-Hexane, diethyl ether, dichloromethane and n-butanol
extracts were prepared from methanol extract with
solvent partitioning and chlorogenic acid, rutin and
conduritol F were isolated from n-BuOH extract with
various chromatographic methods. Structures of the
compounds were elucidated by extensive 1D- and 2D- NMR spectroscopic analysis in combination with IR, MS
experiments. Conduritol F was isolated for the first time
from Tanacetum species. Quantitative analyses of
chlorogenic acid and rutin in MEOH extract were determined with High Pressure Liquid Chromatography
(HPLC). Antioxidant activities of isolated compounds
were studied and chlorogenic acid was found to be
more active than rutin and methanol extract.

Keywords

Tanacetum zahlbruckneri, Chlorogenic acid, Rutin, Conduritol F, HPLC

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