NORMAL, FAZLA KİLOLU VE OBEZ BİREYLERDE IZUMO-1 SPERM YÜZEY RESEPTÖRÜNÜN EKSPRESYON DÜZEYİNİN KARŞILAŞTIRILMASI
Abstract
Çalışmamızda normal, fazla kilolu ve obez bireylerde sperm kalite düzeyleri arasındaki farklılıkları, sperm-yumurta füzyonunda görev alan İzumo-1 sperm yüzey reseptörünün ekspresyon düzeylerini ve artmış Beden Kitle İndeksi (BKİ)’nin sperm DNA’sı üzerine olası etkilerini belirlemek amaçlanmıştır. Çalışmada, 18-35 yaş aralığında, BKİ normal (n=20), fazla kilolu(n=19) ve obez (n=18) erkek bireylerden alınan semen örnekleri kullanıldı. Sperm örnekleri Diff Quik boyama yöntemi ile boyanarak morfolojik kriterleri açısından değerlendirildi. İzumo-1 sperm yüzey reseptörünün ekspresyon düzeylerini belirlemek için western blot analizi kullanıldı. Sperm DNA hasarlarını belirlemek amacıyla yüksek alkali şartlarda tek hücre jel elektroforezi yöntemi kullanıldı. Gruplara ait olan sperm örneklerinde, ortalama sperm konsantrasyonu, sperm motilite oranı ve normal morfolojiye sahip sperm oranlarının özellikle de obez bireylerde düştüğü gözlendi. Morfolojik hasarların, obez bireylerden fazla kilolu ve normal kilolu bireylere doğru azaldığı belirlendi. İzumo-1 sperm yüzey reseptörü ekspresyon düzeylerine bakıldığında, en yüksek İzumo-1 ekspresyonu obez grubunda bulunmaktaydı (p>0.05). Gruplardaki DNA hasarı incelendiğinde comet parametreleri, sperm morfolojik değerlendirme sonuçları ile uyumlu olarak diğer gruplara göre obez grubunda DNA hasarının anlamlı derece arttığını gösterdi (p<0.001). Sonuç olarak çalışmamızda, BKİ’nin erkek infertilitesi ve bununla ilişkili olan sperm sayısı ve morfolojisi ya da sperm DNA hasarı ile İzumo-1 protein ekspresyonu, ilişkili olduğu gösterilmiştir.
Supporting Institution
Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi
Project Number
TYL-2015-6180
References
- KAYNAKLAR 1. Erdemir F. The evaluation of the relationship between obesity and male ınfertility. J Clin Anal Med 2013; 4: 76-82. 2. Isomaa B, Almgren P, Tuomi T, et al. Cardiovascular morbidity and mortality associated with the metabolic syndrome. Diabetes Care 2001; 24: 683-689. 3. Bellver J, Busso C, Pellicer A, et al. Obesity and assisted reproductive technology outcomes. Reprod Biomed Online 2006; 12: 562-568. 4. Tekelioğlu M. Özel Histoloji İnce Yapı ve Gelişme (1. Baskı), Ankara Üniversitesi Tıp Fakültesi Yayınları, Ankara, 2002: 231-244. 5. Pacey AA. Environmental and lifestyle factors associated with sperm DNA damage. Hum Fertil 2010; 13: 189-193. 6. Agarwal A, Allameneni SSR. The effect of sperm DNA damage in assisted reproduction outcomes. Mineva Ginecologia 2004; 56: 235-245. 7. Kruger TF, Acosta AA, Simmons KF, et al. Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil and Steril 1988; 49: 112-117. 8. Hill JO, Wyatt HR, Reed GW, Peters JC. Obesity and the environment: where do we go from here?. Science 2003; 29: 853-855. 9. Sarıozkan S, Canturk, F, Yay A, Akcay A. The effect of different storage temperature on sperm parameters and DNA damage in liquid stored new zealand rabbit spermatozoa. Kafkas Univ Vet Fak Derg 2012; 18: 475–480. 10. Altunkaynak BZ, Özbek E. Obezite: nedenleri ve tedavi seçenekleri. Van Tıp Dergisi 2006; 13: 138-142. 11. Moragianni VA, Jones S-ML, Ryley DA. The effect of body mass index on the outcomes of first assisted reproductive technology cycles. Fertil and Steril 2012; 98: 102-108. 12. Host E, Lindenberg S, Smidt-Jensen S. The role of DNA strand breaks in human spermatozoa used for IVF and ICSI. Acta Obstet Gynecol Scand 2001; 79: 559- 563. 13. Sadler T. Langman's Medical Embryology (5th Ed), William And Wilkins, Baltimore, 1985: 7-83. 14. Fernandez CD, Bellentani FF, Fernandes GS. Diet-induced obesity in rats leads to a decrease in sperm motility. Reproductive Biology and Endocrinology 2011: 9: 32. 15. Palmer NO, Fullston T, Mitchell M, et al. SIRT6 in mouse spermatogenesis is modulated by diet-induced obesity. Reprod Fertil Dev 2011; 23: 929–939. 16. Aggerholm AS, Thulstrup AM, Toft G, et al. Is overweight a risk factor for reduced semen quality and altered serum sex hormone profile ? Fertil and Steril 2008; 90: 619-626. 17. Huang CC, Cheng Lin DP, Tsao HM, et al. Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates. Fertility and Sterility 2005; 84: 130-140. 18. Jensen TK, Andersson AM, Jørgensen N, et al. Body mass index in relation to semen quality and reproductive hormones among 1,558 Danish men. Fertil and Steril 2004; 82: 863-870. 19. Akamine EH, Marçal AC, Camporez JP, et al. Obesity induced by high-fat diet promotes insulin resistance in the ovary. Journal of Endocrinology 2010; 20: 65- 74. 20. Hammiche F, Laven JS, Boxmeer JC, et al. Sperm quality decline among men below 60 years of age undergoing IVF or ICSI treatment. J Androl 2011; 32: 70– 76. 21. Inoue N, Ikawa M, Isotani A, Okabe M. The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs. Nature 2005; 434: 234-238. 22. La Vignera S, Condorelli RA, Vicari E, Calogero AE. Negative effect of increased body weight on sperm conventional and nonconventional flow cytometric sperm parameters. J Androl 2012; 33: 53–58. 23. Inoue N, Hamada D, Kamikubo H, et al. Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. Development 2013; 140: 3221-3229. 24. Sermondade N, Faure C, Fezeu L, et al. BMI in relation to sperm count: an updated systematic review and collaborative meta-analysis. Human Reproduction Update 2012; 19: 221-231. 25. Sallmén M, Sandler DP, Hoppin JA, et al. Reduced fertility among overweight and obese men. Epidemiology 2006; 17: 520-523. 26. Stanton RA. Nutrition problems in an obesogenic environment. Med J Aust 2006; 184: 76-79. 27. El-Melegy NT, Ali M. Apoptotic markers in semen of infertile men: Association with cigarette smoking. International Braz J Urol 2011; 37: 495-506. 28. Junquerıa LC, Carneiro J. Temel Histoloji, Editör çeviri: Aytekin Y, Solakoglu S, Nobel Tıp Kitapbevleri, Ankara, 2006: 431-438. 29. Cavkaytar S, Batioglu S, Gunel M, et al. Genetic evaluation of severe male factor infertility in Turkey: a cross-sectional study. Human Fertility 2012; 15: 100-106.
THE CONTRAST OF THE EXPRESSION LEVEL IN IZUMO-1 SPERM RECEPTORS FOR NORMAL, OVERWEIGHT AND OBESE PERSON
Abstract
In this study it was aimed to determine; the differences between the sperm qualities of normal, over weight and obese person, the expression levels of the Izumo-1 sperm receptor which is assigned in sperm-egg fusion and the possible effects of the increased body mass index (BMI) on the sperm DNA. Semen samples from normal (n=20), over weight (n=19) and obese (n=18) male subject saged 18-35, were used in the study. Sperm samples were stained with Diff Quick staining method and evaluated for morphological criteria. Western blot analysis was used to determine the expression level of Izumo-1. The alkaline comet assay method was used to determine the sperm DNA damages. Mean sperm concentration, sperm motility ratio and sperm rates with normal morphology fell in obese subjects in the sperm samples. Morphological damages were found to decrease from obese individual stoover weight and normal weight individuals.
The highest level of Izumo-1 sperm receptor expression was found in the obese group (p>0.05). Comet parameters showed significant increase in DNA damage in obese group compared to other groups (p<0.001). In conclusion, our study showed that BMI correlates with male infertility and associated sperm count and morphology or sperm DNA damage with Izumo-1 protein expression.
Project Number
TYL-2015-6180
References
- KAYNAKLAR 1. Erdemir F. The evaluation of the relationship between obesity and male ınfertility. J Clin Anal Med 2013; 4: 76-82. 2. Isomaa B, Almgren P, Tuomi T, et al. Cardiovascular morbidity and mortality associated with the metabolic syndrome. Diabetes Care 2001; 24: 683-689. 3. Bellver J, Busso C, Pellicer A, et al. Obesity and assisted reproductive technology outcomes. Reprod Biomed Online 2006; 12: 562-568. 4. Tekelioğlu M. Özel Histoloji İnce Yapı ve Gelişme (1. Baskı), Ankara Üniversitesi Tıp Fakültesi Yayınları, Ankara, 2002: 231-244. 5. Pacey AA. Environmental and lifestyle factors associated with sperm DNA damage. Hum Fertil 2010; 13: 189-193. 6. Agarwal A, Allameneni SSR. The effect of sperm DNA damage in assisted reproduction outcomes. Mineva Ginecologia 2004; 56: 235-245. 7. Kruger TF, Acosta AA, Simmons KF, et al. Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil and Steril 1988; 49: 112-117. 8. Hill JO, Wyatt HR, Reed GW, Peters JC. Obesity and the environment: where do we go from here?. Science 2003; 29: 853-855. 9. Sarıozkan S, Canturk, F, Yay A, Akcay A. The effect of different storage temperature on sperm parameters and DNA damage in liquid stored new zealand rabbit spermatozoa. Kafkas Univ Vet Fak Derg 2012; 18: 475–480. 10. Altunkaynak BZ, Özbek E. Obezite: nedenleri ve tedavi seçenekleri. Van Tıp Dergisi 2006; 13: 138-142. 11. Moragianni VA, Jones S-ML, Ryley DA. The effect of body mass index on the outcomes of first assisted reproductive technology cycles. Fertil and Steril 2012; 98: 102-108. 12. Host E, Lindenberg S, Smidt-Jensen S. The role of DNA strand breaks in human spermatozoa used for IVF and ICSI. Acta Obstet Gynecol Scand 2001; 79: 559- 563. 13. Sadler T. Langman's Medical Embryology (5th Ed), William And Wilkins, Baltimore, 1985: 7-83. 14. Fernandez CD, Bellentani FF, Fernandes GS. Diet-induced obesity in rats leads to a decrease in sperm motility. Reproductive Biology and Endocrinology 2011: 9: 32. 15. Palmer NO, Fullston T, Mitchell M, et al. SIRT6 in mouse spermatogenesis is modulated by diet-induced obesity. Reprod Fertil Dev 2011; 23: 929–939. 16. Aggerholm AS, Thulstrup AM, Toft G, et al. Is overweight a risk factor for reduced semen quality and altered serum sex hormone profile ? Fertil and Steril 2008; 90: 619-626. 17. Huang CC, Cheng Lin DP, Tsao HM, et al. Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates. Fertility and Sterility 2005; 84: 130-140. 18. Jensen TK, Andersson AM, Jørgensen N, et al. Body mass index in relation to semen quality and reproductive hormones among 1,558 Danish men. Fertil and Steril 2004; 82: 863-870. 19. Akamine EH, Marçal AC, Camporez JP, et al. Obesity induced by high-fat diet promotes insulin resistance in the ovary. Journal of Endocrinology 2010; 20: 65- 74. 20. Hammiche F, Laven JS, Boxmeer JC, et al. Sperm quality decline among men below 60 years of age undergoing IVF or ICSI treatment. J Androl 2011; 32: 70– 76. 21. Inoue N, Ikawa M, Isotani A, Okabe M. The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs. Nature 2005; 434: 234-238. 22. La Vignera S, Condorelli RA, Vicari E, Calogero AE. Negative effect of increased body weight on sperm conventional and nonconventional flow cytometric sperm parameters. J Androl 2012; 33: 53–58. 23. Inoue N, Hamada D, Kamikubo H, et al. Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. Development 2013; 140: 3221-3229. 24. Sermondade N, Faure C, Fezeu L, et al. BMI in relation to sperm count: an updated systematic review and collaborative meta-analysis. Human Reproduction Update 2012; 19: 221-231. 25. Sallmén M, Sandler DP, Hoppin JA, et al. Reduced fertility among overweight and obese men. Epidemiology 2006; 17: 520-523. 26. Stanton RA. Nutrition problems in an obesogenic environment. Med J Aust 2006; 184: 76-79. 27. El-Melegy NT, Ali M. Apoptotic markers in semen of infertile men: Association with cigarette smoking. International Braz J Urol 2011; 37: 495-506. 28. Junquerıa LC, Carneiro J. Temel Histoloji, Editör çeviri: Aytekin Y, Solakoglu S, Nobel Tıp Kitapbevleri, Ankara, 2006: 431-438. 29. Cavkaytar S, Batioglu S, Gunel M, et al. Genetic evaluation of severe male factor infertility in Turkey: a cross-sectional study. Human Fertility 2012; 15: 100-106.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Research Article |
| Authors | |
| Project Number | TYL-2015-6180 |
| Publication Date | November 30, 2020 |
| Submission Date | May 17, 2020 |
| Published in Issue | Year 2020 Volume: 29 Issue: 3 |