Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods

Year 2019, Volume: 5 Issue: 1, 148 - 158, 04.01.2019

Zeliha Türkyılmaz , Ahmet Hatipoğlu Mahmut Yüksel , Nurettin Aydoğdu , Gülara Hüseyinova

https://doi.org/10.18621/eurj.421296

Cited By: 1

Abstract

Objectives: Liver transplantation is increasingly being used in
the treatment of end-stage liver disease. Ischemia-reperfusion injury is one of
the major problems encountered in transplantation. In this study, we aimed to
compare the effects of melatonin, pentoxifylline, and dimethyl sulfoxide (DMSO), in hepatic ischemia-reperfusion injury with different methods
such as biochemical/ultrastructural changes and hepatobiliary scintigraphy.

Methods: Thirty rabbits were used in the Laboratory of
Experimental Animals of Trakya University under appropriate conditions. Sham
laparotomy and only ischemia reperfusion group were planned. They were used
melatonin, pentoxifylline, and DMSO after ischemia-reperfusion in the other three groups. 6
rabbits were randomly selected for each group. Rabbits in all groups were
subjected to liver scintigraphy. Following scintigraphy, 2 cm² of
liver tissue was removed to examining for liver antioxidant enzyme levels (superoxide
dismutase [SOD] and glutathione peroxidase [GPx]) and for liver electron
microscopy.

Results: Pentoxifylline and melatonin protected significantly
uptake and excretion functions in liver scintigraphy. When the effects of all
three substances were examined by electron microscopy, it was found that the
three substances protected the liver from the effects of ischemia-reperfusion damage
at varying rates. All three agents were found to protect SOD and GPx from
falling in various amounts.

Conclusions: Studies to prevent ischemia-reperfusion injury, which
may develop as a result of the Pringle maneuver applied to liver
transplantations as well as to liver resections or liver injuries, still
maintain their popularity. In our study, the effects of agents were identified
in three different ways. Ischemia-reperfusion injury-reducing effect of
pentoxifylline gave parallel results with three methods. 

Keywords

Pentoxifylline, melatonin, dimethyl sulfoxide, ischemia, reperfusion

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