Türkiye ve Romanya kökenli Pseudomonas aeruginosa izolatlarının antibiyotik dirençlerinin, biyofilm oluşumu ve genotiplerinin araştırılması

Year 2022, Volume: 33 Issue: 2, 1 - 6, 23.12.2022

Merve Gizem Sezener , Volkan Enes Ergüden , Arzu Findik , Serhan Akgöz , Timur Gülhan , Oana–alexandra Moţco (cıocan) , Alper Çiftci

https://doi.org/10.35864/evmd.1166225

Abstract

Pseudomonas aeruginosa çeşitli hastalık olgularından ve hastane infeksiyonlarından sıklıkla izole edilmektedir. Çoklu antibiyotik direnç özelliği ve biyofilm üretimine sahip olması nedeniyle klinik açıdan önem arz etmektedir. Bu çalışmada Türkiye ve Romanya’da pnömoni bulguları gösteren köpeklerden alınan bronşiyal aspirat örneklerinden izole edilen P. aeruginosa suşlarının biyofilm üretiminin belirlenerek antibiyotiplendirme ve genotiplendirilmelerinin yapılması amaçlanmıştır. Bu amaçla Türkiye ve Romanya izolatı 10’ar adet P. aeruginosa izolatının biyofilm özelliği Kongo red agar yöntemi ile belirlendi. İzolatların antibiyotik direnç durumları Kirby-Bauer Disk Difüzyon Metodu ile belirlendi ve direnç profiline bağlı olarak antibiyotiplendirme yapıldı. Genotipik yakınlıklar RAPD-PCR ile belirlendi. Çalışmada incelenen tüm izolatların biyofilm üretimi yönünden pozitif olduğu saptandı. Antibiyogram sonucunda Türkiye izolatlarının enrofloksasin, seftriakson, amoksisilin + klavulanik asit, siprofloksasin, meropenem, kolistin, gentamisin ve azitromisin içeren disklerle karşı direnç durumları sırasıyla %30, %70, %100, %40, %30, %10, %10, %100 ve Romanya izolatlarının da sırasıyla %30, %60, %100, %30, %10, %0, %10 ve %100 olduğu belirlendi. Antibiyotiplendirme sonucunda, Türkiye ve Romanya izolatlarının %86-100 arasında benzerlik gösterdiği belirlendi. Filogenetik değerlendirme ile Türkiye ve Romanya izolatlarının filogenotipik benzerliklerinin %47-96 arasında olduğu belirlendi. Sonuç olarak, izolatların antibiyotik direncinin yüksek olduğu ve biyofilm üretimine bağlı olarak tedavide kullanılan antibiyotiklere karşı direnç şekillenmiş olabileceği kanısına varıldı.

Keywords

Antibiyotiplendirme, Biyofilm, Genotiplendirme, Pseudomonas aeruginosa

Investigation of the antibiotic resistances, biofilm formation, and genotypes of Pseudomonas aeruginosa isolates from Turkey and Romania

Abstract

Pseudomonas aeruginosa is frequently isolated from various disease and nosocomial infections. It is clinically important because of its multiple antibiotic resistance and biofilm production. The aims of this study were to determine the production of biofilm and to make antibiotyping and genotyping of P. aeruginosa strains isolated from bronchial aspirate samples of dogs with pneumonia in Turkey and Romania. For this purpose, biofilm properties of 10 Turkish and 10 Romanian isolates were determined by Congo red agar method. The resistance profiles of the isolates were determined by Kirby-Bauer Disk Diffusion Method. The antibiotyping was performed according to the resistance profiles of the isolates. Genotypical similarities were determined by RAPD-PCR. In terms of biofilm production, all of the isolates examined in this study were found to be positive. In consequence of the antibiograms, the resistance rates against enrofloxacin, ceftriaxone, amoxicillin + clavulanic acid, ciprofloxacin, meropenem, colistin, gentamicin and azithromycin were stated as 30%, 70%, 100%, 40%, 30%, 10%, 10%, 100% for Turkish isolates and 30%, 60%, 100%, 30%, 10%, 0%, 10%, 100% for Romanian isolates, respectively. As a result of antibiotyping, similarities of the isolates from Turkey and Romania were determined to be between 86-100%. Phylogenotypical similarities of the isolates from Turkey and Romania were determined to be between 47-96%. It was concluded that antibiotic resistances of isolates were high and resistance against antibiotics used in the treatment could be formed due to biofilm production.

Keywords

Antibiotyping, Biofilm, Genotyping, Pseudomonas aeruginosa.

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