The Protective Effects of S-Methyl Methionine Sulfonium Chloride on Brain Tissue Damage in D-Galactosamine-Induced Hepatotoxicity

Year 2022, Volume: 12 Issue: 2, 38 - 43, 22.08.2022

Bertan Boran Bayrak , Dastagul Mahmarzayeva , İsmet Burcu Türkyılmaz , Özlem Saçan , Refiye Yanardağ

https://doi.org/10.26650/experimed.1085407

Abstract

Objective: The objective of the current work is to examine the protective effects of S-methyl methionine sulfonium chloride (MMSC) on brain in galactosamine (GalN)-induced hepatotoxicity in rats.

Materials and Methods: A total of twenty two female Sprague-Dawley rats were randomly assigned into four groups as follows: Group I (n=5), intact control animals; Group II (n=6), animals that received 50 mg/kg/day of MMSC by gavage technique for 3 consecutive days; Group III (n=5), animals injected with a single dose of 500 mg/kg of GalN intraperitoneally (i.p.); and Group IV (n=6) are animals injected with the same dose of GalN (i.p.) 1 hour after MMSC treatment. At the end of the experiments (after 6 hours of the last GalN treatment), all animals were sacrificed under anaesthesia, and brain tissues were dissected out.

Results: A statistically remarkable increase in lipid peroxidation, hydroxyproline, and nitric oxide levels, was detected while a notable decline in the activities of sodium/potassium ATPase was observed in GalN group in comparison with control rats. In contrast, all alterations observed were reversed when MMSC was given to GalN groups.

Conclusion: Consequently, it may be considered that MMSC has a protective role on brain in GalN-induced hepatotoxicity in rats

Keywords

Galactosamine, S-methyl methionine sulfonium chloride, brain, antioxidant effect, oxidative stress

References

• 1. Singh G, Sharma R. Animal models for hepatoxicity. In: Sobti RC, editor. Advances in Animal Experimentation and Modeling. Aca-demic Press: London; 2022.p.195-203. google scholar
• 2. Rouf A, Das AK, Khatoon MSTM, Mitu NL, Shahriar M. Evaluation of hepatoprotective effects of arogyavardhini against D-galac-tosamine-induced hepatotoxicity in rats. J Pharmacog Phyto-chem 2021; 10(1): 12-9. google scholar
• 3. Dong W, Song E, Song Y. Co-administration of lipopolysaccharide and D-galactosamine induces genotoxicity in mouse liver. Sci Rep 2021; 11: 1733. [CrossRef] google scholar
• 4. Hajam YA, Rani R, Ganie SY, Sheikh TA, Javaid D, Qadri SS, et al. Oxidative stress in human pathology and aging: molecular mech-anisms and perspectives. Cells 2022; 11(3): 552. [CrossRef] google scholar
• 5. Anwar MM. Oxidative stress-a direct bridge to central nervous system homeostatic dysfunction and Alzheimer's disease. Cell Biochem Funct 2022; 40(1): 17-27. [CrossRef] google scholar
• 6. Choi EH, Lee SB, Lee DY, Kim GT, Shim SM, Park TS. Increased in-testinal absorption of vitamin U in steamed graviola leaf extract activates nicotine detoxification. Nutrients 2019; 11(6): 1334. [CrossRef] google scholar
• 7. Oktay S, Bayrak G, Alev B, Ipekci H, Ustundag UV, Turkyilmaz IB, et al. The effect of vitamin U on the lung tissue of pentylenetera-zole-induced seizures in rats. Naunyn-Schmiedeberg's Arch Phar-macol 2018; 391(2): 177-84. [CrossRef] google scholar
• 8. Gezginci-Oktayoglu S, Turkyilmaz IB, Ercin M, Yanardag R, Bolkent S. Vitamin U has a protective effect on valproic acid-induced renal damage due to its anti-oxidant, anti-inflammatory, and anti-fi-brotic properties. Protoplasma 2016; 253(1): 127-35. [CrossRef] google scholar
• 9. Bayrak BB, Catal T, Oztay F, Yanardag R, Bolkent S. Efficacy of anti-oxidant vitamins (vitamin C, vitamin E, beta-carotene) and seleni-um supplement on D-galactosamine-induced lung injury. IUFS J Biol 2016; 75(1): 11-8. google scholar
• 10. Ledwozyw A, Michalak J, Stepien A, Kadziolka A. The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clin Chim Acta 1986; 155: 275-83. [CrossRef] google scholar
• 11. Reddy GK, Enwemeka CS. A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem 1996; 29: 22529. [CrossRef] google scholar
• 12. Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophoto-metric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 2001; 5: 62-71. [CrossRef] google scholar
• 13. Ridderstap AS, Bonting SL, Na+-K+-activated ATPase and exocrine pancreatic secretion in vitro. Am J Physiol 1969; 217: 1721-7. [CrossRef] google scholar
• 14. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measure-ment with the Folin phenol reagent. J Biol Chem 1951; 193: 26575. [CrossRef] google scholar
• 15. Jaganjac M, Cindric M, Jakovcevic A, Zarkovic K, Zarkovic N. Lipid peroxidation in brain tumors. Neurochem Int 2021; 149: 105118. [CrossRef] google scholar
• 16. Guglielmotto M, Tamagno E, Danni O. Oxidative stress and hypox-ia contribute to Alzheimer's disease pathogenesis: two sides of the same coin. Sci World J 2009; 9(1): 781-91. [CrossRef] google scholar
• 17. Albaugh VL, Mukherjee K, Barbul A. Proline precursors and colla-gen synthesis: biochemical challenges of nutrient supplementa-tion and wound healing. J Nutr 2017; 147(11): 2011-7. [CrossRef] google scholar
• 18. Freitas A, Aroso M, Barros A, Fernandez M, Conde-Sousa E, Leite M, et al. Characterization of the striatal extracellular matrix in a mouse model of Parkinson's disease. Antioxidants (Basel) 2021; 10(7): 1095. [CrossRef] google scholar
• 19. Qiu B, Wei F, Sun X, Wang X, Duan B, Shi C, et al. Measurement of hydroxyproline in collagen with three different methods. Mol Med Rep 2014; 10(2): 1157-63. [CrossRef] google scholar
• 20. Ganai AA, Ganaie IA, Verma N, Farooqi H. Regression of fibrosis/ cirrhosis by glycine propionyl-l-carnitine treatment in D-galac-tosamine induced chronic liver damage. Chemico-Biol Interact 2016; 260: 117-28. [CrossRef] google scholar
• 21. Ramesh KSV, Swetha P, Nirnmal M, Alla RK. Nitrous oxide - "Double edged sword". Trends Biomater Artific Organs 2014; 28(1): 37. google scholar
• 22. Morales-Medina JC, Aguilar-Alonso P, Di Cerbo A, Iannitti T, Flores G. New insights on nitric oxide: focus on animal models of schizo-phrenia. Behav Brain Res 2021; 409: 113304. [CrossRef] google scholar
• 23. Paradise WA, Vesper BJ, Goel A, Waltonen JD, Altman KW, Haines GK, et al. Nitric oxide: perspectives and emerging studies of a well known cytotoxin. Int J Mol Sci 2010; 11(7): 2715-45. [CrossRef] google scholar
• 24. Monica FZ, Bian K, Murad F. The endothelium-dependent nitric oxide-cGMP pathway. Adv Pharmacol 2016; 77: 1-27. [CrossRef] google scholar
• 25. Subedi L, Gaire BP, Parveen A, Kim SY. Nitric oxide as a target for phytochemicals in anti-neuroinflammatory prevention therapy. Int J Mol Sci 2021; 22(9): 4771. [CrossRef] google scholar
• 26. Abdel-Salam OME, Youness ER, Mohammed NA, Morsy SMY, Omara EA, Slemm AA. Citric acid effects on brain and liver oxida-tive stress in lipopolysaccharide-treated mice. J Med Food 2014; 17(5): 588-98. [CrossRef] google scholar
• 27. Esmekaya MA, Tuysuz MZ, Tomruk A, Canseven AG, Yücel E, Ak-tuna Z, et al. Effects of cell phone radiation on lipid peroxidation, glutathione and nitric oxide levels in mouse brain during epileptic seizure. J Chem Neuroanat 2016; 75: 111-5. [CrossRef] google scholar
• 28. Lichtstein D, Ilani A, Rosen H, Horesh N, Singh SV, Buzaglo N, et al. Na+, K+-ATPase signaling and bipolar disorder. Int J Mol Sci 2018; 19(8): 2314. [CrossRef] google scholar
• 29. Clausen MV, Hilbers F, Poulsen H. The structure and function of Na,K-ATPase isoforms in health and disease. Front Physiol 2017; 8: 371. [CrossRef] google scholar
• 30. Arnaiz GRL. Na+, K+-ATPase in the brain: structure and function. In: Lajtha A, Reith MEA, editors. Handbook of Neurochemistry and Molecular Neurobiology. Springer: Boston MA; 2007.p.209-24. google scholar