Investigation of the Effect of Serum IL-1β Levels on Atherosclerosis: A Turkish Population-Based Study

Year 2023, Volume: 13 Issue: 2, 142 - 147, 18.09.2023

Dilveen Dlawer Omer , Fatma Tuba Akdeniz , Seda Güleç , Zerrin Barut , Deryanaz Billur , Ayça Türer Cabbar , Turgay İsbir

https://doi.org/10.26650/experimed.1246321

Abstract

Objective: Atherosclerosis, as a cardiovascular disease associated with chronic inflammatory conditions and involving a large number of risk factors, harbors a lot of unknowns. Evidence has emerged that almost all stages of the disease are intertwined with inflammation. It has not been fully elucidated in which stages and how interleukin-1 beta (IL-1β), which is associated with inflammations, plays a role in the chronic inflammatory process of the disease. Also, the effect on atherosclerosis progression and biomarker candidacy of IL-1β in the Turkish population is unknown. The aim of this study was to investigate the serum IL-1β expression levels and whether it is associated with changes in risk factors that develop due to the disease in the Turkish population.
Materials and Methods: ELISA method was used to analyze the serum IL-1β levels. Statistical data was obtained utilizing the SPSS v.23 software.
Results: No linkage evidence was found between the serum IL-1β and atherosclerosis (p>0.05). However, the serum IL-1β levels were significantly associated with gender, smoking status, and diabetes history within the study population were significantly associated (p=0.050). According to the lipid profile result, the triglyceride value was found to be significantly decreased in atherosclerosis patients when compared to healthy individuals (p=0.012). Additionally, factors including, gender, smoking status, and history of diabetes were significantly associated with serum cholesterol level (p=0.015).
Conclusion: Although no correlation was found between the serum IL-1β levels of the study groups, it was observed that the serum IL-1β expression alters in patients with atherosclerosis depending on age. In addition, low triglyceride levels may be a marker of disease in atherosclerosis patients. Studies involving different ethnic groups with a large sample and identifying the stages of the disease are necessary to confirm these results.

Keywords

Atherosclerosis, inflammation, IL-1β, triglyceride, lipid level, serum

References

• 1. Worthley SG, Osende JI, Helft G, Badimon JJ, Fuster V. Coronary artery disease: pathogenesis and acute coronary syndromes. Mt Sinai J Med 2001; 68(3): 167-81. google scholar
• 2. Bolton E, Rajkumar C. The ageing cardiovascular system. Rev Clinical Gerontol 2010; 21(2): 99–109. google scholar
• 3. Taleb S. Inflammation in atherosclerosis. Arch Cardiovasc Dis 2016; 109(12): 708–15. google scholar
• 4. Wang JC, Bennett M. Aging and atherosclerosis: mechanisms, functional consequences, and potential therapeutics for cellular senescence. Circ Res 2012; 111(2): 245-59. google scholar
• 5. Calvert PA, Liew TV, Gorenne I, Clarke M, Costopoulos C, Obaid DR, et al. Leukocyte telomere length is associated with highrisk plaques on virtual histology intravascular ultrasound and increased proinflammatory activity. Arterioscler Thromb Vasc Biol 2011; 31(9): 2157–64. google scholar
• 6. Mai W, Liao Y. Targeting IL-1β in the treatment of atherosclerosis. Front Immunol 2020; 11: 589654. google scholar
• 7. Libby P. Interleukin-1 Beta as a target for atherosclerosis therapy: Biological basis of CANTOS and beyond. J Am Coll Cardiol 2017; 70(18): 2278-89. google scholar
• 8. Kirii H, Niwa T, Yamada Y, Wada H, Saito K, Iwakura Y, et al. Lack of interleukin-1beta decreases the severity of atherosclerosis in ApoE-deficient mice. Arterioscler Thromb Vasc Biol 2003; 23(4): 656–60. google scholar
• 9. Abbate A, Van Tassell BW, Biondi-Zoccai GG. Blocking interleukin-1 as a novel therapeutic strategy for secondary prevention of cardiovascular events. Biodrugs 2012; 26(4): 217–33. google scholar
• 10. Vromman A, Ruvkun V, Shvartz E, Wojtkiewicz G, Santos Masson G, Tesmenitsky Y, et al. Stage-dependent differential effects of interleukin-1 isoforms on experimental atherosclerosis. Eur Heart J 2019; 40(30): 2482–91. google scholar
• 11. Galea J, Armstrong J, Gadsdon P, Holden H, Francis SE, Holt CM. Interleukin-1 beta in coronary arteries of patients with ischemic heart disease. Arterioscler Thromb Vasc Biol 1996;16(8): 1000–6. google scholar
• 12. Dewberry R, Holden H, Crossman D, Francis S. Interleukin-1 receptor antagonist expression in human endothelial cells and atherosclerosis. Arterioscler Thromb Vasc Biol 2000; 20(11): 2394–400. google scholar
• 13. Nakamura K, Miyoshi T, Yunoki K, Ito H. Postprandial hyperlipidemia as a potential residual risk factor. J Cardiol 2016; 67(4): 335–9. google scholar
• 14. Di Iorio A, Ferrucci L, Sparvieri E, Cherubini A, Volpato S, Corsi A, et al. Serum IL-1β levels in health and disease: a population-based study.‘The InCHIANTI study’. Cytokine 2003; 22(6): 198-205. google scholar
• 15. Joung KH, Kim JM, Choung S, Lee JH, Kim HJ, Ku BJ. Association between IL-1beta and cardiovascular disease risk in patients with newly diagnosed, drug-naïve type 2 diabetes mellitus: a crosssectional study. Ann Transl Med 2020; 8(5): 225. google scholar
• 16. Hansson GK. Inflammation, atherosclerosis, and coronary artery disease. N Engl J Med 2005; 352(16): 1685–95. google scholar
• 17. Wang M, Zhang J, Jiang LQ, Spinetti G, Pintus G, Monticone R, et al. Proinflammatory profile within the grossly normal aged human aortic wall. Hypertension 2007; 50(1): 219–27. google scholar
• 18. Starr ME, Saito M, Evers BM, Saito H. Age-associated increase in cytokine production during systemic inflammation-II: The role of IL-1β in age-dependent IL-6 upregulation in adipose tissue. J Gerontol A Biol Sci Med Sci 2015; 70(12): 1508-15. google scholar
• 19. Rauchhaus M, Koloczek V, Volk H, Kemp M, Niebauer J, Francis DP, et al. Inflammatory cytokines and the possible immunological role for lipoproteins in chronic heart failure. Int J Cardiol 2000; 76(2–3): 125–33. google scholar
• 20. Kozdag G, Ertas G, Emre E, Akay Y, Celikyurt U, Sahin T, et al. Low serum triglyceride levels as predictors of cardiac death in heart failure patients. Tex Heart Inst J 2013; 40(5): 521-8. google scholar