The Effect of Arbuscular Mycorrhizal Fungus (AMF) on Zucchini yellow mosaic virus (ZYMV) in Summer Squash Cultivars

Year 2015, Volume: 44 Issue: 1-2-3, 11 - 21, 30.01.2016

Semra Demir , H. Murat Sipahioğlu

Abstract

In this study, the effect of Arbuscular Mycorrhizal Fungi (AMF) was
investigated on the growth someparameters of squash (Cucurbita pepo L.)
(morphological and physiological) and
yellow mosaic disease caused by Zucchini yellow mosaic potyvirus (ZYMV) which has been known as an important problem of squash cultivation  leading yield losses. Under controlled
conditions, three squash cultivars (Comet F₁, Focus F₁
and Sakız) were inoculated with four different AMF strains (Gigaspora
margarita, Glomus mossease,Glomus
intraradices and a commercial AMF) in order to determine the most
appropriate combination of squash cultivar and AMF species. Considering
colonization rate and mycorrhizal dependency,the most appropriate combination
was determined between Sakız and commercial AMF, Focus F₁andcommercial
AMF, and Comet F₁ andGlomus
mosseae. These combinations were used in the subsequent experiment. At the
end of the study, it was found that the application of AMF was stimulated plant
growth positively. Generally, chlorophyll contents were decreased in plants
inoculated with AMF and ZYMV compared to control plants. The phosphorus content
was not statistically significant between the applications. It was determined
that the disease severity was increased in mycorrhizal plants compared with
non-mycorrhizal plants. On the other hand the virus concentration was decreased
in AMF and ZYMV pathosystem.

Keywords

Summer squash, Arbuscular Mycorrhizal Fungi (AMF), Zucchiniyellow mosaicpotyvirus (ZYMV)

References

• Anonim, 2010.Türkiye İstatistik Kurumu. http://www.tuik.gov.tr/ (Erişim tarihi: 03.01.2012). Blua, M. J., T.M. Perring, 1989. Effect of zucchini yellow mosaic virus on development and yield of cantaloupe (Cucumis melo). Plant Disease73: 317-320. Brunt, A., K. Crabtree, M. Dallwitz, A. Gibbs, L. Watson, 1996.Virus of Plants. Cab International Wallingfort. 1484p. Çığşar, S., N. Sarı, İ. Ortaş, 2000.Hıyarda vesküler-arbusküler mikorizanın bitki büyümesi ve besin maddeleri alımı üzerine etkileri.Turkish Journal of Agriculture and Forestry24: 571-578. Daft, M.J., B.O. Okusanya, 1973. Effect of Endogene mycorrhiza on plant growth. V. Influence of infection on the multiplication of viruses in tomato, petunia and strawberry. Phytopatology 72: 975-983. Davis, R.M., J.A. Menge, D.C. Erwin, 1979. Influence of Glomus fasciculatus and soil phosphorus on Verticillium Wilt of cotton. Phytopathology 69: 453 - 456. Declerck, S., C. Plenchette, D.G. Strullu, 1995.Mycorrhizal dependency of banana (Musa acuminate, AAA group) cultivar.Plant Soil176: 183-187. Dehne, H.W., 1982. Interactions between vesicular - arbuscular mycorrhizal fungi and plant pathogens. Phytopathology 72: 1115 - 1119. Demir, S., 1998. Bazı Kültür BitkilerindeVesiküler-Arbusküler Mikorhiza (VAM) Oluşumu ve Bunun Bitki Gelişimi ve Dayanıklılıktaki Rolü Üzerine Araştırmalar. Ege Üniversitesi Fen Bilimleri Enstitüsü, (Basılmamış), Bornova, İzmir, 144 s. Demir, S., 2002. Arbusküler Mikorhizal Fungus (AMF) Glomus intraradices (Schenck&Smith)’in bazı sebze bitkilerinde oluşumu ve kolonizasyonu. YYÜ Ziraat Fakültesi Tarım Bilimleri Dergisi 12(1): 53-57. Demir, S.,A. Akköprü, 2007. Using of Arbuscular Mycorrhizal Fungi (AMF) for biocontrol of soil-borne fungal plant pathogens. Pages 17-37 in: Biological Control of Plant Diseases.S.B. Chincholkar and K.G. Mukerji, eds. Haworth Press, NY, USA. FAOSTAT, 2010. Statistic Database. http://apps.fao.org/ (Erişim tarihi: 14.01.2012). Foissac, X., M.G. Edwards, J.P. Du, J.A. Gatehouse, 2002. Putative protein digestion in a sap-sucking hom opteran plant pest (rice brown plant hopper; Nilaparvata lugens: Delphacidae): identification of tripsin-like and cathepsin B-like proteases. Insect Biochemistry and Molecular Biology32: 967-978. Giovanetti, M., B. Mosse, 1980. An evaluation of techniques for measuring vesicular- arbuscular mycorrhizal infection in roots. New Phytologist84: 489- 500. Hayman, D., 1982. Influence of soils and fertility on activity and survial vesicular-arbuscular mycorrhizal fungi. Phytopathology 72: 1119–1126. Jabaji-Hare, S. H., L. W. Stobbs, 1984. Electron microscopic examination of tomato roots coinfected with Glomus sp. and tobacco mosaic virus. Phytopathology74: 277-279. Kacar, B., 1984. Bitki Besleme Uygulama Klavuzu. Ankara Üniversitesi Ziraat Fakültesi Yayınları, 70 s. Lecoq, H.,J. M., Lemaire, C. Wipf-Scheibel, 1991. Control of Zuccihini yellow mosaic virus in squash by cross protection. Plant Disease75: 208-211. Linderman, R.G., A. E., Davis, 2004. Varied response of marigold (Tagetes spp.) genotypes to inoculation with different arbuscular mycorrhizal fungi.ScientiaHorticulturae99: 67-78. Lisa, V., G. Boccardo, G.D’Agostino, G.Delavalle, M. D’Aquilio, 1981.Characterization of a potyvirus that causes zucchini yellow mosaic virus.Phytopathology71: 667-672. Marschner, H., 1995. Mycorrhizas.Pages 566-595 in: Mineral Nutrition of Higher Plants.H. Marschner, ed. Academic Press, London. Mignouna, H.D., M. M. Abang, K.R. Green, R. Asiedu, 2001.Inheritance of resistance in water yam (Dioscorea alata) to anthracnose (Colletotrichum gleosporoides). Theoretical Applied Genetics Journal, 103: 52–55. Phillips, J.M., D.S. Hayman, 1970. Improved procedure for cleaning roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assement of infection.Transactions of the British Mycological Society55: 158-161. Rhodes, L.H. 1980. Theuse of mycorrhizae in crop production systems.Outlook on Agriculture10 (6): 275 – 281. SAS, 1998. SAS/STAT Software: Hangen and Enhanced. Sas, Ins. Inc. Cri.NCI. Schönbeck, F., 1980.Endomycorrhiza in relation to plant diseases. Pages 271-280 in: Soil – Borne Pathogens, B. Schippers and W. Gams eds. Academic Pres, New York, NY. Sensoy, S. S. Demir, O. Turkmen, C. Erdinc, O.B. Savur, 2007. Responses of some different pepper (Capsicum annuum L.) genotypes to inoculation with two different arbuscular mycorrhizal fungi.Scientia Horticulturae113: 92-95. Sensoy S., S. Demir, S. Tufenkci, C. Erdinc, E. Demirer Durak. H. Ünsal, G. Halifeoglu, A. Ekincialp, 2011. Response of four hybrid zucchini (Cucurbita pepo L.) cultivars to different arbuscular mycorrhizal fungi.The Journal of Animal &Plant Sciences21(4): 751-757. Shaul, O., S. Galili, H. Volpin, I. Ginzberg, Y. Elad, I. Chet, Y. Kapulnik Y., 1999. Mycorrhiza-induced changes in disease severity and PR protein expression in tobacco leaves. Molecular Plant-Microbe Interactions12: 1000-1007. Sipahioğlu, H.M., S. Demir, M. Usta, A. Akköprü, 2009. Biological relationship of Potato virus Y and arbuscular mycorrhizal fungus Glomus intraradices in potato.Pest Technology3(1):63-66. Smith, J.H.C., A. Benitez, 1955. Chlorophylls analysis in plant materials. Pages 159 in: Modern Methods of Plant Analysis, K. Paeeh and M.V. Traceyeds. Smith, S.E., D.J. Read, 2008. Mineral nutrition, toxic element accumulation and water relations of arbuscular mycorrhizal plants. Pages 145-148 in: Mycorrhizal Symbiosis,Smith, S.E., D.J. Read eds., Academic Press, London. Takashi, T., N. Katano, N. Yoshikama, 1994.Evidence for vesicular-arbuscular mycorrhizae infection in viroid-infected root tissues. Z Pflanzenkr Pflanzenschutz, 101: 267-271. Trimble, R.M., N.R. Knowles, 1994. Influence of phosphorus nutrition and vesicular-arbuscular mycorrhizal fungi on growth and yield of greenhouse cucumber (Cucumis sativus L.). Canadian Journal of Plant Science,75: 251-259. Van der Heijden, M.G.A., T. Boller, A. Wiemken, I.R. Sanders, 1998. Differentarbuscular mycorrhizal fungal species are potential determinants of plant community structure. Ecology79 (6): 2082-2091. Yuan, C., D.E. Ullman, 1996. Comparison of efficency and propensity as measures of vector importance in zucchini yellow mosaic potyvirus transmission by Aphis gossypii and A. craccivora.Phytopathology86: 698-703.