Türkiye Şeker Pancarı Üretim Alanlarında Beet virus Q’nun Yaygınlığı

Year 2017, Volume: 46 Issue: 2, 53 - 60, 30.12.2017

Ebru Erkan Nazlı Dide Kutluk Yılmaz

Abstract

Şeker pancarı (Beta vulgaris L.) plasmodiophorid vektör Polymyxa betae tarafından taşınan Beet necrotic yellow vein virus (BNYVV), Beet soil-borne virus (BSBV), Beet soil-borne mosaic virus (BSBMV) ve Beet virus Q (BVQ) gibi toprak kökenli virüslerden etkilenmektedir. Genellikle BVQ’nun, BNYVV ve BSBV ile birlikte bulunduğu belirtilmektedir. Bu sebeple, Türkiye’nin 31 ilinden coğrafik orjinlerine göre seçilmiş BNYVV ve/ya da BSBV ile bulaşık olduğu bilinen 82; P. betae ile bulaşık 21 toprak örneği tuzak bitki testinde kullanılmıştır. Bu örneklerde BVQ’nun bulunma durumu RNA-1’e spesifik primerler kullanılarak RT-PCR ile araştırılmıştır. 103 örneğin 91’inde BVQ için pozitif reaksiyon elde edilmiştir. Bu çalışma, Gaziantep ili hariç, incelenen 30 ilin tamamında BVQ’nun oldukça yaygın (% 88.35) olduğunu göstermiştir. Bu çalışma, Türkiye’de BVQ’nun varlığının ilk kaydı niteliğindedir.

Keywords

Şeker pancarı, BVQ, RNA-1, RT-PCR

Prevalence of Beet Virus Q in Sugar Beet Production Areas of Turkey

Abstract

Sugar beet (Beta
vulgaris L.) is affected by soil-borne viruses transmitted by the plasmodiophorid
vector Polymyxa betae such as Beet necrotic yellow vein virus (BNYVV),
Beet soil-borne virus (BSBV), Beet soil-borne mosaic virus (BSBMV) and
Beet virus Q (BVQ). BVQ has often
been reported with BNYVV and BSBV. Therefore, 82 soil samples known to be
infested with BNYVV and/or BSBV while 21 soil samples infested with P. betae were selected on the basis of
their geographical locations in 31 provinces in
Turkey and used
in bait plant tests. The presence of BVQ was
investigated by RT-PCR using the primers specific to RNA-1 segment. Ninety
one of the 103 soil samples gave positive reaction for BVQ. The study showed
that BVQ was very common (88.35%) in all provinces except Gaziantep. To our
knowledge, this is the first report of the occurrence of BVQ in Turke

Keywords

Sugar beet, BVQ, RNA-1

References

• Borodynko, N., 2006. First report of Beet virus Q in Poland. Plant Disease, 90: 1363.
• Borodynko, N., Rymelska, N., Hasiow-Jaroszewska, B., Pospieszny, H., 2009. Molecular characterization of three soil-borne sugar beet-infecting viruses based on the coat protein gene. Journal of Plant Pathology, 91 (1): 191-193.
• FAOSTAT, 2014. Food and Agriculture Organization Statistics Division. http://www.fao.org/faostat/en/#data/QC (Erişim Tarihi: 17 Temmuz 2017)
• Farzadfar, S., Pourrahim, R., Golnaraghi, A. R., Ahoonmanesh, A., 2005. First report of beet virus Q on sugar beet in Iran. Plant Disease, 89: 1359.
• Farzadfar, Sh., Pourrahim, R., Golnaraghi, A. R., Ahoonmanesh, A., 2007. Surveys of Beet necrotic yellow vein virus, Beet soil-borne virus, Beet virus Q and Polymyxa betae in sugar beet fields in Iran. Journal of Plant Pathology, 89 (2): 277-281.
• Ivanovic, M., Macfarlane, I., Woods, R. D., 1983. Viruses of sugar beet associated with Polymyxa betae. Ann. Rep. Rothamstead Exp. Stn., 189-190.
• Koenig, R., Pleij, C. W. A., Beier, C., Commandeur, U., 1998. Genome properties of Beet virus Q, a new furo-like virus from sugar beet, determined from unpurified virus. Journal of General Virology, 79: 2027-2036.
• Kutluk Yılmaz N. D., Yanar, Y., Günal, H., Erkan, S., 2004a. Effects of Soil Properties on the Occurence of Beet Necrotic Yellow Vein Virus and Beet Soilborne Virus on Sugar Beet in Tokat, Turkey. Plant Pathology Journal, 3 (2): 56-60.
• Kutluk Yılmaz, N., Meunier, A., Schmit, J. F., Stas, A., Bragard, C., 2004b. Tokat ili şeker pancarı üretim alanlarında Beet necrotic yellow vein virus, Beet soilborne virus, Beet virus Q ve vektör Polymyxa betae KESKIN’in multiplex reverse transcription-PCR yöntemi ile belirlenmesi. Türkiye I. Bitki Koruma Kongresi, 8-10 Eylül 2004, Samsun, s.174.
• Kutluk Yilmaz N. D., Arli Sokmen, M., 2010. Occurrences of sugar beet soilborne viruses transmitted by Polymyxa betae Northern and Central Parts of Turkey. Journal of Plant Pathology, 92 (2): 497-500.
• Kutluk Yilmaz, N. D., Arli Sokmen, M., Kaya, R., Sevik, M. A., Tunali, B., Demirtas, S., 2016. The widespread occurrences of Beet soil-borne virus and RNA-5 containing Beet necrotic yellow vein virus isolates in sugar beet production areas in Turkey. European Journal of Plant Pathology, 144: 443-455.
• Lesemann, D. E., Koenig, R., Lindsten, K., Henry, C., 1989. Serotypes of beet soil-borne furovirus from FRG and Sweden. Bulletin OEPP/EPPO Bulletin, 19: 539-540.
• Mehrvar, M., 2009. Diversity of soil-borne sugar beet viruses in Iran: a comprehensive study of Beet necrotic yellow vein virus, Beet black scorch virus and other pomoviruses in Iran. Ph.D. Thesis. Universite catholique de Louvain, Belgium, 160 pp.
• Meunier, A., Schmit, J. F., Stas, A., Kutluk, N., Bragard, C., 2003. Multiplex reverse transcription for simultaneous detection of Beet Necrotic Yellow Vein Virus, Beet Soilborne Virus, and Beet Virus Q and their vector Polymyxa betae KESKIN on sugar beet. Applied and Environmental Microbiology, 69 (4): 2356-2360.
• Pavli, O., Prins, M., Skaracis, G. N., 2010. Detection of Beet soil-borne virus and Beet virus Q in sugar beet in Greece. Journal of Plant Pathology, 92 (3): 793-796.
• Ratti, C., Bianchi, L., Resca, R., De Biaggi, M., Harju, V. A., Henry, C. M., Jackeviviene, E., Cvjetkivic, B., Rubies Autonell, C., 2005. Incidence of sugar beet soil-borne viruses in sugar beet centuries. In: Rush, C. M. (Ed.) Proceedings of the 6th Symposium of the International Working Group on Plant Viruses with Fungal Vectors, 5-7 September 2005, Bologna, pp. 165-168.
• Rubies Autonell, C., Ratti, C., Resca, R., 2006. First report of Beet virus Q in Spain. Plant Disease, 90: 110.
• Rysanek, p., Homa, I. H., Zouhar, M., 2006. Study of sugar beet viruses transmitted by Polymyxa betae in the Czech Republic. Matica Srpska Proceedings for Natural Science, 110: 47-54.
• Stas, A., Meunier, A., Schmit, J. F., Bragard, C., 2001. First report of Beet virus Q in Belgium. Plant Disease, 85: 1288.
• Şiray A. 1990. Şeker Pancarı Tarımı. Pankobirlik Yayınları, No: 2, Ankara, 128 s.
• Torrance, L., 2008. Pomoviruses. in: Encylopedia of Virology. B. W. J. Mahy and M. H. V. Van Regenmortel, eds. Academic Press, Elsevier.