İZOLE SIÇAN MİYOMETRİYUMUNDA METABOLİK İNHİBİSYONA BAĞLI KONTRAKTİL BASKILANMADA FOSFOKREATİN/ KREATİN KİNAZ ENERJİ SİSTEMİNİN ROLÜNÜN İNCELENMESİ

Year 2008, Volume: 7 Issue: 1, 78 - 83, 30.11.2008

Mete Özcan , Ergül Alçın Tuğrul Kuzgun Ahmet Ayar

Abstract

Bu
çalışmada, izole sıçan miyometriyumunda, kasılabilirlik yönünden hipoksiye
adaptasyon kapasitesinde Kreatin kinaz (CK)/fosfokreatin (PCr) enerji sisteminin olası rolünün incelenmesi
amaçlanmıştır.

 

Gebe
olmayan erişkin Wistar cinsi dişi sıçanlardan izole edilen miyometriyum
kesitleri, izole organ banyosu-na yerleştirildi ve test ajanları eklenerek;
kontraksiyonların frekansı, amlitüd ve kasılma gücü üzerine olan etkileri, 10
dakikalık periyotlar süresince, veriler normalize edilerek değerlendirildi.
Bulguların istatistiksel değerlendirilmesinde ANOVA varyans analizi testi
kullanıldı.

 

KCN
(0.5mM) ile oluşturulan metabolik stres, spontan kontraksiyonları kademeli
başlayan bir etki ile tama-men inhibe etti. Kasılma eğrisi altında kalan alan
(spontan %100 ), KCN uygulamasını takiben %51.2±9.6 oranında baskılarken; PCr (5 mM) uygulamasını takiben
kontrol değerlerinin %58.4±7.6 (p > 0.05 n=7), CK uygulamasını takiben ise
%62± 7.2 (p > 0.05, n=7) düzeyine kadar derlendi.

 

CK/PCr
enerji sisteminin, metabolik inhibisyona bağlı miyometriyum kasılma gücünde
meydana gelen zayıflamaya kısmen aracılık ettiğini, ancak bunun kasılmayı
sekteye uğratan tek sorumlu mekanizma olmadığını düşündürmektedir.

Keywords

Miyometriyum, Kasılma, Metabolik Stres, Kretin Kinaz, Rat

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