THYMOQUİNONE’NİN İNSAN MEME KANSERİ HÜCRE HATLARI ÜZERİNE ETKİLERİ

Year 2010, Volume: 9 Issue: 1, 63 - 67, 28.10.2010

Hüseyin Timurkan

Abstract

Thymoquinone (TQ), çörek otunun aktif
maddesi olup, birçok kanser türlerinin tedavisinde kullanılmaktadır. Fakat
insan meme kanseri hücre hatlarında henüz bir çalışma bulunmamaktadır.
Dolayısıyla bu çalışmada; TQ’nın insan meme kanseri meme hatlarına etkisinin
olup olmadığının araştırılması amaçlanmıştır. Çalışmada; İnsan meme
adenocarcinoma hücreleri (MCF–7) Amerikan kültür koleksiyonundan (ATCC) elde
edilerek kullanıldı.  Thymoquinone n
serisel dilüsyonları hazırlanarak mililitrede 1x10⁵ hücre olacak
şekilde 24 kuyucuklu kültür peleytlerinde 37⁰C’de bir gece inkübe
edilerek, böbrek epitelyum hücreleri ile karşılaştırıldı. Sonuçlarımızda 250 µM
konsantrasyona kadar TQ’nın toksin etki göstermediği, MCF-7’nin 250 µM’da
üremesini engellediği, fakat bu konsantrasyonda böbrek epitelyum hücrelerine
etki etmediği gözlemlendi. Bulgularımız; TQ’nin hücre siklusunu durdurarak
kanser hücrelerini öldürdüğünü göstermektedir. 250 µM konsantrasyonda kanser
ücresi olmayan hücrelerde ise dayanıklılığı da görüldü.

Keywords

Thymoquinone, Çörek otu, antitümöral aktivite, Meme hücre hattı

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