Cytotoxicity evaluation of a new self-adhering flowable composite by dentin barrier test

Year 2013, Volume: 30 Issue: 3, 140 - 144, 11.10.2013

Hayriye Esra Ülker , Mustafa Ülker Erhan Özcan

Abstract

OBJECTIVE: The aim of this study was to evaluate the cytotoxicity of a new self-adhering flowable composite (Vertise Flow) in a dentin barrier test device using three-dimensional cultures of bovine dental pulp derived cells.

MATERIALS AND METHOD: Artificial cell culture perfusion chambers were separated into two compartments using a 500 µm dentin disc. In the lower compartment, a pulp chamber was created by placing the three-dimensional cultures onto the dentin disc. Test materials were introduced into the upper compartment in direct contact with the cavity side of the dentin discs following the manufacturers’ directions. Negative and positive controls were President and Vitrebond, respectively. Subsequently, the pulpal part of the perfusion chamber containing the cell culture was perfused with a culture medium. After an exposure period of 24 hours, cell survival was determined by using the MTT assay. Statistical analyses were performed using the Kruskal-Wallis and Mann Whitney U tests.

RESULTS: The difference in survival rates between Vertise Flow and Vitrebond groups (positive control) was statistically significant (p<0.05). However, the difference in survival rates between Vertise Flow and President groups (negative control) was not statistically significant (P>0.05).

CONCLUSION: Vertise Flow and the negative control group showed similar effects on the survival of the bovine dental pulp-derived cells. Toxic substances released from Vertise Flow may not influence the pulpal cells in case the residual dentin layer is at least 0.5 mm thick.

Keywords

Cytotoxicity, Dental Adhesives, Dental Pulp, Dentin Barrier Test, Self-Adhering Flowable Composite

Kendinden bağlanabilen yeni bir akışkan kompozitin sitotoksisitesinin dentin bariyer testi ile değerlendirilmesi

Abstract

AMAÇ: Bu çalışmanın amacı kendinden bağlanabilen yeni
bir akışkan kompozitin (Vertise Flow) sitotoksisitesini,
sığır dental pulpasından elde edilmiş hücrelerin üç-boyutlu
kültürlerinin kullanıldığı bir dentin bariyer test düzeneği ile
değerlendirmektir.
GEREÇ VE YÖNTEM: Yapay hücre kültürü perfüzyon odaları
500 µm kalınlığındaki dentin diskleri ile iki kompartımana
ayrıldı. Alt kompartımanda, üç-boyutlu hücre kültürleri dentine
temas edecek şekilde yerleştirilerek pulpa odası kısmı
oluşturuldu. Üst kompartımanda ise test materyalleri,
dentin diskinin kavite kısmı üzerine üretici firmanın önerdiği
şekilde uygulandı. Negatif ve pozitif kontrol materyalleri
sırası ile President ve Vitrebond idi. Daha sonra, pulpal
kısımdaki hücre kültürlerinin kültür ortamı ile perfüzyonları
sağlandı. Yirmi dört saat sonra hücre canlılığı MTT testi ile
değerlendirildi. Veriler Kruskal Wallis ve Mann Whitney U
testleri kullanılarak istatistiksel olarak analiz edildi.
BULGULAR: Vertise Flow ve Vitrebond (pozitif kontrol) gruplarının
hücre canlılık oranları arasındaki fark istatistiksel
olarak anlamlı idi (p < 0.05). Ancak, Vertise Flow ile President
(negatif kontrol) gruplarının hücre canlılık oranları
arasındaki fark istatistiksel olarak anlamlı değildi (p > 0.05)
SONUÇ: Vertise Flow, sığır dental pulpasından elde edilmiş
hücrelerin canlılığı üzerine negatif kontrol grubuna benzer
bir etki gösterdi. Kalan dentin tabakası kalınlığının en az
0.5 mm olması durumunda, Vertise Flow’dan salınması
muhtemel biyololojik olarak aktif bileşenlerin pulpa hücrelerini
etkilemeyebileceği düşünülmektedir.

Keywords

Dental Adezivler, Dentin Bariyer Testi, Diş Pulpası, Kendinden Bağlanabilen Akışkan Kompozit, Sitotoksisite

References

• Van Meerbeek B, Vargas M, Inoue S, Yoshida Y, Peumans M, Lambrechts P, et al. Adhesives and cements to promote preservation dentistry. Oper Dent 2001;26 Suppl 6:S119-44.
• Van Meerbeek B, De Munck J, Yoshida Y, Inoue S, Vargas M, Vijay P, et al. Buonocore memorial lecture. Adhesion to enamel and dentin: current status and future challenges. Oper Dent 2003;28:215-35.
• Ulker M, Ozcan M, Sengün A, Ozer F, Belli S. Effect of artificial aging regimens on the performance of self-etching adhesives. J Biomed Mater Res B Appl Biomater 2010;93:175-84.
• Ülker M, Belli S. Self-etch adeziv sistemler: Diş sert dokularına bağlanma. SÜ Dişhek Fak Derg 2006;15:116-22.
• Ermiş RB. Günümüzdeki Adezivlerde Teknik Hassasiyet: I. Asitlenen ve Yıkanan Adezivler. Dişhekimliğinde Klinik 2008;23:48-53.
• Ermiş RB. Günümüzdeki Adezivlerde Teknik Hassasiyet: II. Kendinden Asitli Adezivler. Dişhekimi Bilimsel 2008;29:12-6.
• Poss SD. Utilization of a new self-adhering flowable composite resin. Dent Today 2010;29:104-5.
• Rengo C, Goracci C, Juloski J, Chieffi N, Giovannetti A, Vichi A, et al. Influence of phosphoric acid etching on microleakage of a self-etch adhesive and a self-adhering composite. Aust Dent J 2012;57:220-6.
• Vichi A, Margvelashvili M, Goracci C, Papacchini F, Ferrari M. Bonding and sealing ability of a new self-adhering flowable composite resin in class I restorations. Clin Oral Investig 2013;17:1497-506.
• Fu J, Kakuda S, Pan F, Hoshika S, Ting S, Fukuoka A, et al. Bonding performance of a newly developed step-less all-in-one system on dentin. Dent Mater J 2013;32:203-11.
• Bektas OO, Eren D, Akin EG, Akin H. Evaluation of a self-adhering flowable composite in terms of micro-shear bond strength and microleakage. Acta Odontol Scand 2013;71:541-6.
• Korsuwannawong S, Srichan R, Vajrabhaya LO. Cytotoxicity evaluation of self-etching dentine bonding agents in a cell culture perfusion condition. Eur J Dent 2012;6:408-14.
• Schmalz G, Schuster U, Thonemann B, Barth M, Esterbauer S. Dentin barrier test with transfected bovine pulp-derived cells. J Endod 2001;27:96-102.
• Wataha JC. Principles of biocompatibility for dental practitioners. J Prosthet Dent 2001;86:203-9.
• Schmalz G. Concepts in biocompatibility testing of dental restorative materials. Clin Oral Invest 1997;1:154-62.
• Schmalz G. Use of cell cultures for toxicity testing of dental materials--advantages and limitations. J Dent 1994;22 Suppl 2:S6-11.
• Schmalz G, Schuster U, Koch A, Schweikl H. Cytotoxicity of low pH dentin bonding agents in a dentin barrier test in vitro. J Endod 2002;28:188-92.
• Schuster U, Schmalz G, Thonemann B, Mendel N, Metzl C. Cytotoxity testing with three-dimensional cultures of transfected pulp-derived cells. J Endod 2001;27:259-65.
• Wiegand A, Buchholz K, Werner C, Attin T. In vitro cytotoxicity of different desensitizers under simulated pulpal flow conditions. J Adhes Dent 2008;10:227-32.
• International Organization for Standardization. ISO 7405:2008: Evaluation of biocompatibility of medical devices used in dentistry. Geneva: ISO; 2008.
• Vajrabhaya LO, Korsuwannawong S, Bosl C, Schmalz G. The cytotoxicity of self-etching primer bonding agents in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;107:e86-90.
• Ülker HE, Hiller KA, Schweikl H, Seidenader C, Sengun A, Schmalz G. Human and bovine pulp-derived cell reactions to dental resin cements. Clin Oral Investig 2012;16:1571-8.
• Noda M, Wataha JC, Kaga M, Lockwood PE, Volkmann KR, Sano H. Components of dentinal adhesives modulate heat shock protein 72 expression in heat-stressed THP-1 human monocytes at sublethal concentrations. J Dent Res 2002;81:265-9.
• Michelsen VB, Moe G, Skålevik R, Jensen E, Lygre H. Quantification of organic eluates from polymerized resin-based dental restorative materials by use of GC/MS. J Chromatogr B Analyt Technol Biomed Life Sci 2007;850:83-91.
• Hamid A, Hume WR. Diffusion of resin monomers through human carious dentin in vitro. Endod Dent Traumatol 1997;13:1-5.
• Pawlowska E, Poplawski T, Ksiazek D, Szczepanska J, Blasiak J. Genotoxicity and cytotoxicity of 2-hydroxyethyl methacrylate. Mutat Res 2010;696:122-9.