Evaluation of Candida albicans biofilm formation on different soft lining materials

Year 2015, Volume: 32 Issue: 1, 19 - 25, 09.02.2015

Duygu Karakış , Canan Akay Demet Erdönmez Arife Doğan

https://doi.org/10.17214/aot.50404

Cited By: 1

Abstract

OBJECTIVE: The purpose of this study was to evaluate Candida albicans biofilm formation on soft liners prepared against two surfaces with different properties.

MATERIALS AND METHOD: Four soft liners (Molloplast B, Permafleks, Elite-soft, Ufi Gel P) were prepared against glass or dental plaster surfaces (5x5x2 mm; n=5). The surface roughness and contact angle of the soft liner specimens were measured. Candida albicans ATCC 10231 was grown in Sabouraud dextrose broth (SDB) at 37 °C for 24 h. For biofilm formation, the specimens were incubated in polystrene wells together with fresh SDB and the C. albicans culture at 37 °C for 48 h. The specimens were stained with 0.1% crystal violet and dehydrated at 65 °C. The quantification of the C. albicans biofilm formation was performed spectrophotometrically. Comparison of the surface roughness, contact angle and biofilm formation between the materials was done using one way ANOVA and Tukey tests. Correlation between surface roughness, contact angle and biofilm formation on the specimens prepared against glass or plaster surfaces was performed using Pearson correlation analysis.

RESULTS: Specimens prepared against plaster showed significantly higher surface roughness, contact angle and biofilm formation values in comparison to those prepared against glass surface (p<0.05). Among the specimens prepared against the dental plaster surface, the highest and the lowest biofilm formation values were observed in the Ufi Gel P and Molloplast B specimens, respectively. Among the specimens prepared against the glass surface, the greatest biofilm formation was shown for Ufi Gel P. There was no correlation between the surface properties (surface roughness and contact angle) and the amount of biofilm formation for the soft liner materials tested in this study (p>0.05).

CONCLUSION: Although the soft liners tested in this study had similar surface topographic properties, they showed different amounts of C. albicans biofilm formation. Smooth surfaces were found to reduce the biofilm formation.

Keywords

Biofilm, Candida albicans, Polydimethylsiloxanes

Farklı yumuşak astar materyallerinin Candida albicans biyofilm formasyonu açısından değerlendirilmesi

Abstract

AMAÇ: Bu çalışmada iki farklı yüzey yapısına göre
hazırlanan yumuşak astar materyallerinde Candida albicans
biyofilm oluşumunun değerlendirilmesi amaçlanmıştır.
GEREÇ VE YÖNTEM: Dört yumuşak astar materyali (Molloplast
B, Permafleks, Elite-Soft, Ufi Gel P) cam veya alçı yüzeyler
üzerinde hazırlandı (5x5x2 mm; n=5). Tüm örneklerin yüzey
pürüzlülükleri ve temas açıları ölçüldü. Candida albicans
ATCC 10231 Sabouraud dextrose besiyeri (SDB) içerisinde
37 °C’de 24 saat süresince çoğaltıldı. Ardından, biyofilm
oluşumu için örnekler polistren kuyucuklarda, önceden
elde edilen Candida albicans kültürü ve taze SDB ilavesiyle
37 °C’de 48 saat süresince enkübe edildi. Örnekler %0.1
kristal viyole solüsyonu ile boyanarak 65 °C’de kurumaya
bırakıldı. Biyofilm oluşumu spektrofotometrik yöntemle incelendi.
Test edilen materyallerin yüzey pürüzlülüğü, temas
açıları ve biyofilm formasyonlarının karşılaştırılması oneway
ANOVA ve Tukey testi ile yapıldı. Cam veya alçı yüzeylerde hazırlanan örneklerin yüzey pürüzlülüğü, temas
açıları ve biyofilm oluşumu arasındaki korelasyon Pearson
korelasyon analizi ile incelendi.
BULGULAR: Alçı yüzeyinde hazırlanan örneklerde yüzey
pürüzlülüğü, temas açısı ve biyofilm oluşumu, cam üzerinde
hazırlananlara göre istatistiksel olarak daha fazla
bulundu (p 0.05).
SONUÇ: Test edilen yumuşak astar materyalleri, yüzey topografik
özellikleri açısından benzer olsalar da farklı miktarlarda
Candida albicans biyofilm oluşumu gösterdiler.
Düz yüzeylerin biyofilm oluşumunu azalttığı gözlendi.

Keywords

Biyofilm, Kandida albikans, Polidimetilsiloksanlar

References

• Turgut M, Bolayır G, Doğan OM, Keskin S, Doğan A, Boztuğ A. Bond strength of a silicone soft lining material to poly(methyl methacrylate) resin treated with maleic anhydride and its terpolymers. J Adhes 2007;83:927-38.
• Sinobad D, Murphy WM, Huggett R, Brooks S. Bond strength and rupture properties of some soft denture liners. J Oral Rehabil 1992;19:151-60.
• Karacaer Ö, Karaarslan A, Dogan A, Özenci H. Yumuşak astar materyallerinin Candida albicans üzerine etkilerinin değerlendirilmesi. GÜ Diş Hek Fak Der 1994;2:103-7.
• Doğan OM. Yumuşak astar materyallerin klinik endikasyonları. Atatürk Üniv Diş Hek Fak Derg 2005;15:70-5.
• Braden M, Wright PS, Parker S. Soft lining materials--a review. Eur J Prosthodont Restor Dent 1995;3:163-74.
• Bal BT, Yavuzyılmaz H. Yumuşak astar maddeleri. Atatürk Üniv Diş Hek Fak Derg 2006;Suppl 1:53-60.
• Soygun K, Bolayır G, Dogan A, Demir H, Dogan M, Keskin S. Effect of surface treatments on tensile bond strength between a silicone soft liner and heat-cured denture base resin. J Adhes 2011;87:951-65.
• Anusavice KJ. Phillips' science of dental materials. 10th edn. Philadelphia: WB Sanders;1995.
• Demir H, Soygun K, Dogan A, Keskin S, Dogan OM, Bolayir G. Effect of maleic anhydride pretreatment on tensile bond strength of a silicone soft liner to a denture base polymer. J Adhes Dent 2011;13:481-7.
• Noort R. Introduction to dental materials, 2nd edn. St. Louis: Mosby Elsevier; 2002.
• Roth J, Albrecht V, Nitschke M, Bellmann C, Simon F, Zschoche S, et al. Surface functionalization of silicone rubber for permanent adhesion improvement. Langmuir 2008;24:12603-11.
• Mutluay MM, Oğuz S, Ørstavik D, Fløystrand F, Doğan A, Söderling E, et al. Experiments on in vivo biofilm formation and in vitro adhesion of Candida species on polysiloxane liners. Gerodontology 2010;27:283-91.
• Busscher HJ, van der Mei HC. Physico-chemical interactions in initial microbial adhesion and relevance for biofilm formation. Adv Dent Res 1997;11:24-32.
• Yerliyurt K, Nalbant D, Kalkancı A, Kuştimur S. İki farklı silikon esaslı yumuşak astar maddesinin zamana bağlı olarak yüzeylerinden izole edilen C. albicans hücrelerinde ALS1 adezyon geninin ekspresyonundaki değişimin incelenmesi. Gaziosmanpaşa Üniversitesi Tıp Fakültesi Dergisi 2012;4:9-21.
• Garcia LT, Jones JD. Soft liners. Dent Clin North Am 2004;48:709- 20, vii.
• Nikawa H, Jin C, Makihira S, Egusa H, Hamada T, Kumagai H. Biofilm formation of Candida albicans on the surfaces of deteriorated soft denture lining materials caused by denture cleansers in vitro. J Oral Rehabil 2003;30:243-50.
• Puius YA, Scully B. Treatment of Candida albicans pericarditis in a heart transplant patient. Transpl Infect Dis 2007;9:229-32.
• Boscato N, Radavelli A, Faccio D, Loguercio AD. Biofilm formation of Candida albicans on the surface of a soft denture-lining material. Gerodontology 2009;26:210-3.
• Williams DW, Kuriyama T, Silva S, Malic S, Lewis MA. Candida biofilms and oral candidosis: treatment and prevention. Periodontol 2000 2011;55:250-65.
• Waters MG, Williams DW, Jagger RG, Lewis MA. Adherence of Candida albicans to experimental denture soft lining materials. J Prosthet Dent 1997;77:306-12.
• Nevzatoğlu EU, Ozcan M, Kulak-Ozkan Y, Kadir T. Adherence of Candida albicans to denture base acrylics and silicone-based resilient liner materials with different surface finishes. Clin Oral Investig 2007;11:231-6.
• Kang SH, Lee HJ, Hong SH, Kim KH, Kwon TY. Influence of surface characteristics on the adhesion of Candida albicans to various denture lining materials. Acta Odontol Scand 2013;71:241-8.
• Vural C, Ozdemir G, Kurtulmus H, Kumbuloglu O, Ozcan M. Comparative effects of two different artificial body fluids on Candida albicans adhesion to soft lining materials. Dent Mater J 2010;29:206-12.
• Hahnel S, Rosentritt M, Bürgers R, Handel G, Lang R. Candida albicans biofilm formation on soft denture liners and efficacy of cleaning protocols. Gerodontology 2012;29:e383-91.
• Mutluay MM, Oguz S, Fløystrand F, Saxegaard E, Dogan A, Bek B, et al. A prospective study on the clinical performance of polysiloxane soft liners: one-year results. Dent Mater J 2008;27:440-7.
• Burns DR, Burns DA, DiPietro GJ, Gregory RL. Response of processed resilient denture liners to Candida albicans. J Prosthet Dent 1987;57:507-12.
• Morgan TD, Wilson M. The effects of surface roughness and type of denture acrylic on biofilm formation by Streptococcus oralis in a constant depth film fermentor. J Appl Microbiol 2001;91:47-53.
• Verran J, Maryan CJ. Retention of Candida albicans on acrylic resin and silicone of different surface topography. J Prosthet Dent 1997;77:535-9.
• Yamauchi M, Yamamoto K, Wakabayashi M, Kawano J. In vitro adherence of microorganisms to denture base resin with different surface texture. Dent Mater J 1990;9:19-24.
• Pereira-Cenci T, Cury AA, Cenci MS, Rodrigues-Garcia RC. In vitro Candida colonization on acrylic resins and denture liners: influence of surface free energy, roughness, saliva, and adhering bacteria. Int J Prosthodont 2007;20:308-10.
• Tari BF, Nalbant D, Dogruman Al F, Kustimur S. Surface roughness and adherence of Candida albicans on soft lining materials as influenced by accelerated aging. J Contemp Dent Pract 2007;8:18-25.
• Nikawa H, Jin C, Hamada T, Murata H. Interactions between thermal cycled resilient denture lining materials, salivary and serum pellicles and Candida albicans in vitro. Part I. Effects on fungal growth. J Oral Rehabil 2000;27:41-51.
• Taylor RL, Bulad K, Verran J, McCord JF. Colonization and deterioration of soft denture lining materials in vivo. Eur J Prosthodont Restor Dent 2008;16:50-5.
• Wright PS. The effect of soft lining materials on the growth of Candida albicans. J Dent 1980;8:144-51.
• Pavan S, dos Santos PH, Filho JN, Spolidorio DM. Colonisation of soft lining materials by micro-organisms. Gerodontology 2010;27:211-6.