The Evaluation of The Relation Between Nasal Polyp Etiopathogenesis and the Amount of Biofilm

Year 2022, , 36 - 38, 28.02.2022

Kazım Bozdemir , Elif Ersoy Çallıoğlu , Bülent Ulusoy , Selami Candan , Serkan Şerifler , Nurcan Özyurt Koçakoğlu , Yuce İslamoglu

https://doi.org/10.54005/geneltip.1014726

Abstract

Objective: To evaluate the effect of biofilm layer on polyp formation in nasal polyposis by comparing the amount of biofilm layer on polyp tissue and the normal mucosa.
Material and Methods: This study is a prospective study. 14 patients who underwent functional endoscopic sinus surgery (FESS) for nasal polyposis were evaluated. Patients were had no history of previous FESS. Tissue samples were obtained from lower turbinate (Group 1 n:14) and from nasal polyp (Group 2, n:14) of the same nasal cavity. Biofilm presence was identified using scanning electron microscopic (SEM) morphological findings. In biofilm positive samples, the presence of biofilm in less than 25% of the surface area was classified as (+), between 25-50% as (++) , and over 50% as (+++).
Results: In Group 2, with SEM imaging, (+++) biofilm presence was detected in 9 patients and (++) biofilm was detected in 5 patients. In Group 1, no biofilm was detected 8 patients, while; (+++), (++), and (+) biofilm presence was detected in 1, 2, and 3 patients respectively. Significant difference was found between group 1 and group 2 with respect to the amount of biofilm according to SEM (p =0.000).
Conclusion: In samples, statistically significant difference was found between polyp tissue and normal mucosa in terms of the amount of biofilm. However, further studies with larger patient series are required in order to reach a definitive conclusion on the effect of biofilm on pathogenesis of polyp.

Keywords

Biofilm, Nasal Polyp, Microscopy, Electron, Scanning

NAZAL POLİP ETİOPATOGENEZİ VE BİOFİLM MİKTARI ARASINDAKİ İLİŞKİNİN DEĞERLENDİRİLMESİ

Abstract

Amaç: Polip dokusu ve normal mukoza üzerindeki biyofilm tabakası miktarını karşılaştırarak nazal polipoziste biyofilm tabakasının polip oluşumuna etkisini değerlendirmek amaçlanmaktadır.
Materyal ve Metod: Bu çalışma, prospektif bir çalışma olup nazal polipozis nedeniyle fonksiyonel endoskopik sinüs cerrahisi (FESS) uygulanan 14 hasta değerlendirildi. Hastalar, daha önce endoskopik sinüs cerrahisi geçirmemiş hastalardan oluşmaktaydı. Doku örnekleri, aynı nazal kaviteden alt konkadan (Grup 1, n:14) ve nazal polip dokusundan (Grup 2, n:14) alındı. Biyofilm varlığı, Scanning elektron mikroskobu (SEM) morfolojik bulgular kullanılarak tanımlandı. Biyofilm pozitif örneklerde, görüntülenen yüzey alanının %25’inden az sahada biyofilm varlığı (+), %25-50 arası (++) ve %50’den fazla sahada biyofilm varlığı (+++) olarak sınıflandırıldı.
Bulgular: Grup 2’de SEM görüntülemede 9 hastada (+++) biyofilm mevcudiyeti saptanırken 5 hastada (++) biyofilm tespit edildi. Grup 1'de ise 8 hastada biyofilm saptanmazken 1 hastada (+++), 2 hastada (++) ve 3 hastada (+) biyofilm varlığı saptandı. SEM bulgularına göre biyofilm miktarı açısından grup 1 ve grup 2 arasında anlamlı fark bulundu (p=0.000).
Sonuç: Örneklerde, polip dokusu ile normal mukoza arasında biyofilm miktarı açısından istatistiksel olarak anlamlı fark bulundu. Bununla birlikte, biyofilmin polip patogenezine etkisi konusunda kesin bir sonuca varmak için daha geniş hasta serileri ile yapılacak çalışmalara ihtiyaç vardır.

Keywords

Biyofilm, Nazal Polip, Scanning-Electron Mikroskopi

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