Seroprevalence of Brucellosis in Individuals Engaged in Animal Husbandry in Erzurum Region

Year 2023, , 768 - 774, 31.12.2023

Mahmut Ucar , Ahmet Yılmaz , Selahattin Çelebi , Sinan Yılmaz

https://doi.org/10.54005/geneltip.1334740

Abstract

Objective: Brucellosis is one of the most common zoonotic diseases in our country and the world. This study investigated the seroprevalence of brucellosis in individuals who were engaged in animal husbandry in Erzurum region, were over 18 years of age, or did not have any clinical complaints of brucellosis.
Materials and Methods: Our study was conducted on 271 volunteers engaged in livestock farming in 20 districts of Erzurum province between 2020 and 2021. Brucella antibodies were investigated in blood samples using ELISA IgG, ELISA IgM, Rose Bengal, and Standard tube agglutination tests. A questionnaire was administered to the participants to investigate the risk factors of brucellosis.
Results: The mean age of the participants was 43.6 ± 15.7.2 years (min:18, max:90), and there were 137 male (50.6%) and 134 female (49.4%) particpants. Seropositivity was detected with ELISA IgG in 40 of 271 participants (14.8%). Seropositivity was detected in the ELISA IgM test in 2 of these 40 participants (0.7%). Seropositivity was found in 46 participants (17%) in the Rose Bengal and 29 individuals in the STA test. According to the serology results, ELISA IgG positivity was higher in male participants compared to female participants (ratio=1.6). Among pet owners, ELISA IgG seropositivity was found higher than the participants who did not have pets (ratio=1.9). In the evaluation based on educational status, the rate of seropositivity was slightly higher in the participants who were illiterate and had a poor level of education.
Conclusion: In our study, it was determined that the prevalence rate obtained by ELISA IgG was consistent with previous studies conducted in the region. No significant relationship was observed between the serology results of the participants and their gender and educational status. On the other hand, the positivity of ELISA IgG results was higher among the participants who had pets, and there was a significant relationship at the margin.

Keywords

Brucella seroprevalence, Brucellosis, ELISA, Erzurum, Rose Bengal

Thanks

We would like to thank those who participated in this research and to Öğr. Gör. Abdulkadir Gülen and Oğuzhan Tunç for their support in the laboratory studies

Erzurum Yöresinde Hayvancılıkla Uğraşan Kişilerde Brusella Seroprevalansı

Abstract

Amaç: Bruselloz dünyada ve ülkemizde en sık görülen zoonotik hastalıklardan birisidir. Bu araştırma Erzurum bölgesinde hayvancılıkla uğraşan ve bruselloz açısından herhangi bir klinik şikayeti olmayan 18 yaş üstü kişilerde brusella seroprevalansını araştırma amacıyla yapılmıştır.
Yöntem: Çalışmamız 2020-2021 yıllarında Erzurum ilinin 20 ilçesinde hayvancılık faaliyetlerinde bulunan 271 gönüllü kişi üzerinde yürütüldü. Alınan kan örneklerinde Brusella antikorları; ELISA IgG, ELISA IgM, Rose Bengal ve Standart Tüp aglütinasyon testleri kullanılarak araştırıldı. Bruselloz açısından risk faktörlerini araştırmak amacıyla katılımcılara anket formu dolduruldu.
Bulgular: Katılımcıların yaş ortalaması 43.6±15.7.2 yıl (min:18, maks:90) ve erkekler 137(%50.6), kadınlar ise 134 (%49.4) kişi idi. Çalışmamıza katılan 271 kişinin 40’ında ELISA IgG ile seropozitiflik saptanmıştır (%14.8). Bu 40 kişiden iki kişide aynı zamanda ELISA IgM seropozitifliğine rastlanmıştır (%0.7). Rose Bengal ile 46 kişide (%17), STA yöntemi ile 29 kişide seropozitiflik bulunmuştur. Seroloji sonuçlarına göre erkeklerde, kadınlara göre ELİSA IgG pozitifliği yüksek bulunmuştur (oran=1,6). Evcil hayvan besleyenlerde, evcil hayvanı olmayanlara göre ELISA IgG seropozitifiği daha yüksek bulundu (oran=1,9). Eğitim durumuna göre yapılan değerlendirmede okur yazar olmayan ve eğitim seviyesi düşük olan katılımcılarda seropozitiflik oranı biraz daha yüksek bulunmuştur.
Sonuç: Çalışmamızda ELISA IgG ile belirlenen prevalans oranı bölgemizde daha önce yapılmış çalışmalarla uyumlu bulundu. Çalışmamızda cinsiyet ve öğrenim durumu ile katılımcıların seroloji sonuçları arasında anlamlı bir ilişki gözlenmedi. Fakat evcil hayvana sahip katılımcılarda ELISA IgG sonuçlarının pozitifliği daha yüksek ve sınırda anlamlı ilişki söz konusuydu.

Keywords

Brusella seroprevalansı, Bruselloz, ELISA, Erzurum, Rose Bengal

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