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Periferik Sinir Yaralanmalarında Kalsiyum Kanal Blokerleri İle Birlikte Sigara Dumanı Maruziyetinin Sinir İyileşmesine Olan Etkisinin Araştırılması

Year 2022, Volume: 32 Issue: 2, 119 - 124, 29.04.2022
https://doi.org/10.54005/geneltip.972273

Abstract

Amaç: Bu çalışma deneysel olarak oluşturulan siyatik sinir kesisin de sigara dumanı maruziyeti ile birlikte kalsiyum kanal blokerlerinin etkisini araştırmak için planlandı.
Gereç-Yöntem: Tüm gruplarda siyatik sinir tek taraflı kesildi ve 0. saatte onarım yapıldı. Onarım sonrası grup 1 e sadece günde tek doz 1 ml %0.9 serum fizyolojik, grup 2 ye 90 gün 30 dakika sigara dumanı, grup 3 e 1mg/kg Kalsiyum Kanal Blokeri, grup 4 e 90 gün 30 dakika sigara dumanı ve 1mg/kg Kalsiyum Kanal Blokeri verildi. 12. hafta sonunda Yürüme Analizi ve Siyatik Fonksiyon İndeksi (SFİ), miyelinli akson sayıları, akason alanları ve miyelin çapları değerlendirildi.
Bulgular: Ölçülen SFİ değerlerinden grup 1 ile grup 3 ve 4 arasında anlamlı fark olduğu, grup 2 ile grup 3 ve 4 arasında anlamlı fark olduğu tespit edildi (p<0.05). Ayrıca grup 2 deki akson sayısı ile grup 3 teki akson sayısı arasında da anlamlı fark bulundu(p<0.05).
Sonuç: Siyatik fonksiyon indeksi açısından periferik sinir kesilerinde onarım sonrası kalsiyum kanala blokerleri olumlu sigara dumanı da olumsuz yönde etki yapmaktadır. Ayrıca siyatik fonksiyon indeksine göre sigara dumanın yaptığı bu olumsuz yönde etki kalsiyım kanal blokerleri ile giderilebilir. Ancak bu veriler akson sayısı akson alanı ve miyelin çapı ile desteklenmemektedir

Supporting Institution

, T.C. Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğünün

Project Number

11102051

References

  • 1-Mackinnon SE, Dellon A.L, Hudson AR, Hunter DA. Nerve regeneration through a pseudosynovial sheath in a primate model. Plast Reconstr Surg 1985;75:833-41
  • 2-Brett K, Parker R, Wittenauer S, Hayashida K, Young T, Vincler M. Impact of chronic nicotine on sciatic nerve injury in the rat. J Neuroimmunol 2007;186(1-2):37-44
  • 3-Taylor GI, Ham F. The free vascularized nerve graft. A furhter experimental and clinical appilication of microvascular techniques. Plast Recons Surg 1976;57:413.
  • 4-Sewerynek E, Abe M, Reiter RJ, Barlow RL, Chen L, McCabe T, et al. Melatonin administration prevents lipopolysaccharide-induced oxidative damage in phenobarbital – treated animals. J Cell Biochem 1995;58: 436-444
  • 5-Kater SB, Mills LR. Regulation of growth cone behavior by calcium. The Journal of Neuroscience 1991;11: 891-899.
  • 6-Stys PK, Ransom BR, Waxman SG. Effects polyvalent cations and dihydropridine calcium blockers on recovery of CNS white matter from anoxia. Neurosci Lett. 1990;115:293-299.
  • 7-Patro IK, Chattopadhyay M, Patro N. Flunarizine enhances functional recovery following sciatic nerve crush lesion in rats. Neurosci Lett. 1999; 26;97-100.
  • 8-Gairola CG, Wu H, Gupta RC, Diana JN. Mainstream and sidestream cigarette smoke-induced DNA adducts in C7Bl and DBA mice. Environ Health Perspect. 1993;99:253–255.
  • 9- Subramaniam S, Whitsett JA, Hull W, Gairola CG. Alteration of pulmonary surfactant proteins in rats chronically exposed to cigarette smoke. Toxicol Appl Pharmacol. 1996;140:274 –280.
  • 10-Patro IK, Chattopadhyay M, Patro N. Flunarizine enhances functional recovery following sciatic nerve crush lesion in rats. Neurosci Lett. 1999; 26;97-100.
  • 11-Kanaya F, Firrell JC, Breidenbach WC. Sciatic function index, nevre conduction tests, muscle contraction, and axon morphometry as indicators of regeneration. Plast Reconstr Surg 1996;98(7):1264–74
  • 12-Ozmen S, Ayhan S, Latifoğlu O, Siemionow M. Stamp and paper method: a superior technique for the walking track analysis. Plast Reconstr Surg 2002;109(5):1760–1.
  • 13-Bain JR, Mackinnon SE, Hunter DA. Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat. Plast Reconstr Surg 1989;83(1):129–38.
  • 14-Larsen JO. Stereology of nerve cross-sections. J. Neurosci. Methods 1198;85:107–118.
  • 15-Stys PK, Ransom BR, Waxman SG. Effects polyvalent cations and dihydropridine calcium blockers on recovery of CNS white matter from anoxia. Neuroscience Lett 1990;115:293-299.
  • 16-. Zachodne DW, Ho LT, Gross PM. Acute endoneurial ischemia induced by epineurial andothelin in the rat sciatic nerve. Am J Physiol 1992;263:1806 -1810.
  • 17-Van der Zee CE, Schuurman T, Traber J, Gispen WH. Oral administration of nimodipine accelerates functional recovery following peripheral nerve damage in the rat. Neurosci Lett 1987;83(1-2):143-148.
  • 18-Mattsson P, Janson AM, Aldskogius H, Svensson M. Nimodipine promotes regeneration and functional recovery after intracranial facial nerve crush. J Comp Neurol 2001;437(1):106-117.
  • 19-Santafe MM, Garcia N, Lanuza MA, Uchitel OD, Salon I, Tomas J. Decreased calcium influx into the neonatal rat motor nerve terminals can recruit additional neuromuscular. Neuroscience 2002;110(1):147-54.
  • 20-Rinker B, Fink BF, Barry NG, Fife JA, Milan ME, Stoker AR, et al. The effect of cigarette smoking on functional recovery following peripheral nerve ischemia/reperfusion injury. Microsurgery 2011;31:59–65
  • 21-Hart C, Kisro NA, Robinson SL, Ksir C. Effects of the calcium channel blocker nimodipine on nicotine-induced locomotion in rats. Psychopharmacology (Berl) 1996;128:359 –361.
  • 22-Rinker B, Fink BF, Stoker AR, Milan ME, Nelson PT. Calcium Channel Blockers Reduce the Effects of Cigarette Smoking on Peripheral Nerve Ischemia/Reperfusion Injury. Ann Plast Surg 2013;70(2):222-6

Investigation of the Effects of Calcıum Channel Blockers with Cigarette Smoke Exposure to Nerve Healing at Peripheral Nerve Injuries

Year 2022, Volume: 32 Issue: 2, 119 - 124, 29.04.2022
https://doi.org/10.54005/geneltip.972273

Abstract

Objective: A study was planned to investigate the effect of exposure to cigarette smoke and the effects of calcium channel blockers in experimentally induced sciatic nerve injuries. Material-Method: The sciatic nerve was cut on one side in all the groups, and repair was performed in hour 0. After repair, a single daily dose of 1 ml of 0.9% saline was administered to group 1, 90 days, 30 minutes, cigarette smoke to group 2, 1mg/kg calcium channel blocker to group 3, 90 days, 30 minutes, cigarette smoke and 1mg/kg Calcium channel blocker was given to group 4. Results: At the end of the 12th week, gait analysis at and sciatic function index (SFI), the number of myelinated axons, axon areas and myelin diameters were assessed. There was a significant difference between the measured values of SFI in group 1 and group 3 and 4, and there was a significant difference between group 2 and group 3 and 4 (p <0.05). In addition there was a significant difference between the number of axons in group 2 and group 3 (p <0.05). Conclusion: As a result, after peripheral nerve laceration repair calcium channel blockers have a positive effect on the sciatic function index while cigarette smoke has a negative effect. In addition, according to the sciatic function index the negative effect of cigarette smoke can be resolved with calcium channel blockers. But; these data are not supported by the number of axon, axon area, and the myelin diameter

Project Number

11102051

References

  • 1-Mackinnon SE, Dellon A.L, Hudson AR, Hunter DA. Nerve regeneration through a pseudosynovial sheath in a primate model. Plast Reconstr Surg 1985;75:833-41
  • 2-Brett K, Parker R, Wittenauer S, Hayashida K, Young T, Vincler M. Impact of chronic nicotine on sciatic nerve injury in the rat. J Neuroimmunol 2007;186(1-2):37-44
  • 3-Taylor GI, Ham F. The free vascularized nerve graft. A furhter experimental and clinical appilication of microvascular techniques. Plast Recons Surg 1976;57:413.
  • 4-Sewerynek E, Abe M, Reiter RJ, Barlow RL, Chen L, McCabe T, et al. Melatonin administration prevents lipopolysaccharide-induced oxidative damage in phenobarbital – treated animals. J Cell Biochem 1995;58: 436-444
  • 5-Kater SB, Mills LR. Regulation of growth cone behavior by calcium. The Journal of Neuroscience 1991;11: 891-899.
  • 6-Stys PK, Ransom BR, Waxman SG. Effects polyvalent cations and dihydropridine calcium blockers on recovery of CNS white matter from anoxia. Neurosci Lett. 1990;115:293-299.
  • 7-Patro IK, Chattopadhyay M, Patro N. Flunarizine enhances functional recovery following sciatic nerve crush lesion in rats. Neurosci Lett. 1999; 26;97-100.
  • 8-Gairola CG, Wu H, Gupta RC, Diana JN. Mainstream and sidestream cigarette smoke-induced DNA adducts in C7Bl and DBA mice. Environ Health Perspect. 1993;99:253–255.
  • 9- Subramaniam S, Whitsett JA, Hull W, Gairola CG. Alteration of pulmonary surfactant proteins in rats chronically exposed to cigarette smoke. Toxicol Appl Pharmacol. 1996;140:274 –280.
  • 10-Patro IK, Chattopadhyay M, Patro N. Flunarizine enhances functional recovery following sciatic nerve crush lesion in rats. Neurosci Lett. 1999; 26;97-100.
  • 11-Kanaya F, Firrell JC, Breidenbach WC. Sciatic function index, nevre conduction tests, muscle contraction, and axon morphometry as indicators of regeneration. Plast Reconstr Surg 1996;98(7):1264–74
  • 12-Ozmen S, Ayhan S, Latifoğlu O, Siemionow M. Stamp and paper method: a superior technique for the walking track analysis. Plast Reconstr Surg 2002;109(5):1760–1.
  • 13-Bain JR, Mackinnon SE, Hunter DA. Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat. Plast Reconstr Surg 1989;83(1):129–38.
  • 14-Larsen JO. Stereology of nerve cross-sections. J. Neurosci. Methods 1198;85:107–118.
  • 15-Stys PK, Ransom BR, Waxman SG. Effects polyvalent cations and dihydropridine calcium blockers on recovery of CNS white matter from anoxia. Neuroscience Lett 1990;115:293-299.
  • 16-. Zachodne DW, Ho LT, Gross PM. Acute endoneurial ischemia induced by epineurial andothelin in the rat sciatic nerve. Am J Physiol 1992;263:1806 -1810.
  • 17-Van der Zee CE, Schuurman T, Traber J, Gispen WH. Oral administration of nimodipine accelerates functional recovery following peripheral nerve damage in the rat. Neurosci Lett 1987;83(1-2):143-148.
  • 18-Mattsson P, Janson AM, Aldskogius H, Svensson M. Nimodipine promotes regeneration and functional recovery after intracranial facial nerve crush. J Comp Neurol 2001;437(1):106-117.
  • 19-Santafe MM, Garcia N, Lanuza MA, Uchitel OD, Salon I, Tomas J. Decreased calcium influx into the neonatal rat motor nerve terminals can recruit additional neuromuscular. Neuroscience 2002;110(1):147-54.
  • 20-Rinker B, Fink BF, Barry NG, Fife JA, Milan ME, Stoker AR, et al. The effect of cigarette smoking on functional recovery following peripheral nerve ischemia/reperfusion injury. Microsurgery 2011;31:59–65
  • 21-Hart C, Kisro NA, Robinson SL, Ksir C. Effects of the calcium channel blocker nimodipine on nicotine-induced locomotion in rats. Psychopharmacology (Berl) 1996;128:359 –361.
  • 22-Rinker B, Fink BF, Stoker AR, Milan ME, Nelson PT. Calcium Channel Blockers Reduce the Effects of Cigarette Smoking on Peripheral Nerve Ischemia/Reperfusion Injury. Ann Plast Surg 2013;70(2):222-6
There are 22 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Original Article
Authors

Muhammed Nebil Selimoğlu

Mustafa Raşid Toksöz

Abit Aktaş

Funda Yiğit

Nedim Savacı This is me

Project Number 11102051
Publication Date April 29, 2022
Submission Date July 29, 2021
Published in Issue Year 2022 Volume: 32 Issue: 2

Cite

Vancouver Selimoğlu MN, Toksöz MR, Aktaş A, Yiğit F, Savacı N. Investigation of the Effects of Calcıum Channel Blockers with Cigarette Smoke Exposure to Nerve Healing at Peripheral Nerve Injuries. Genel Tıp Derg. 2022;32(2):119-24.

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