KOLON KANSERİ KÖK HÜCRELERİNDE AKTEOSİD’İN İNFLAMASYON VE/YADA APOPTOZA ETKİSİ VAR MIDIR?

Year 2022, Volume: 32 Issue: 4, 372 - 379, 31.08.2022

Fatma Fırat , Canan Türkoğlu Feyzan Ozdal Kurt , Hafize Seda Vatansever

https://doi.org/10.54005/geneltip.1053439

Cited By: 1

Abstract

AMAÇ: Kolon kanseri dünya çapında ana sağlık problemlerinin başında gelmektedir. Kanser kök hücreleri (CSC'ler), tümör heterojenitesi ve dormansisinde yer alan tümör başlatan hücreler olarak adlandırılır. CSC'ler ilaç direncine, metastaza ve primer ve metastatik kanserlerin nüksetmesine neden olabilir. Kolon kanser kök hücrelerinin digger hücreler ile olan etkileşimlerive hayatta kalma eğilimleri etkili tedavi için alternatif bir yol olabilir. Çalışmamızda primer (HCT-116) ve metastatik (Colo-741) kolon CSC'lerinde, asteosidin kök hücre özelliği, apoptotik ve inflamatuar sürece etkilerini değerlendirmeyi amaçladık.
GEREÇ VE YÖNTEM: CSC' ler, anti-CD133 belirteci kullanılarak MINIMACS sistemi ile her iki tip kolon kanseri hücre hattından elde edildi. Metastatik Colo-741 ve non-metastatik HCT-116 CD133+ ve CD133- hücreleri, 48 saat boyunca Acteosid olmadan veya Acteosid ile culture edildi. Apoptoz için Kaspaz-3, Bcl, Bax, ve Fas-L, inflamasyon için IL-1β, TNF-α, IL-6, IL8 ve IL-10 proteinlerinin ifadesi indirekt immünositokimya tekniği ile H-Score yapılarak analiz edildi. Hücre morfolojisindeki değişiklikler invert mikroskop altında incelendi.
BULGULAR VE SONUÇ: Oct-4 ifadesinin hem metastatic hem de metastatik olmayan kolon kanser hücrelerinde akteosit uygulamasından sonra azaldığı görüldü. Ayrıca Colo-741 CD133+ ve CD133- hücrelerin her ikisinde de Bax/Bcl oranının yoğunluğunu arttırdı ve kaspaz 3 ifadesinin arttığı görüldü. Akteosid metastatic COLO-741 hücrelerinde inflamasyona immunohistokimyasal olarak etki etmezken, apoptotic sürece Bax/Bcl oranını değiştirerek katkıda bulunmuştur. Hücrelerin morfolojisi değerlendirildiğinde de COLO-741 CD133+ ve CD133- hücrelerde apoptotic hücrelerin sayılarının arttığı görülmüştür.

Keywords

Kolon kanseri kök hücreleri, COLO-741, HCT-116, apoptoz, inflamasyon, acteosit.

Supporting Institution

Mali destek alınmamıştır

Is Acteoside Effects on Colon Cancer Stem Cells Via Inflamation or Apoptosis?

Abstract

AIM: Colon cancer is one of the main health problems worldwide. Cancer stem cells (CSCs) are referred to as tumor-initiating cells involved in tumor heterogeneity and dormancy. CSCs can cause drug resistance, metastasis, and recurrence of primary and metastatic cancers. The interactions and survival trends of colon cancer stem cells with other cells may be an alternative route for effective treatment. In our study, we aimed to evaluate the effects of asteoside on stem cell properties, apoptotic and inflammatory processes in primary (HCT-116) and metastatic (Colo-741) colon CSCs.
METHERIALS AND METHODS: CSCs were obtained from both types of colon cancer cell lines with the MINIMACS system using the anti-CD133 reagent. Metastatic Colo-741 and non-metastatic HCT-116 CD133+ and CD133- cells were cultured with or without Acteoside for 48 hours. Expressions of Caspase-3, Bcl, Bax, and Fas-L for apoptosis, and IL-1β, TNF-α, IL-6, IL8 and IL-10 for inflammation were analyzed by indirect immunocytochemistry technique by performing H-Score. Changes in cell morphology were examined under an inverted microscope.
RESULTS AND CONCLUSION: It was observed that Oct-4 expression decreased after acteocyte administration in both metastatic and non-metastatic colon cancer cells. In addition, Colo-741 increased the intensity of the Bax/Bcl ratio in both CD133+ and CD133- cells and increased caspase 3 expression. While acteoside did not immunohistochemically affect inflammation in metastatic COLO-741 cells, it contributed to the apoptotic process by changing the Bax/Bcl ratio. When the morphology of the cells was evaluated, it was observed that the number of apoptotic cells increased in COLO-741 CD133+ and CD133- cells.

Keywords

Colon cancer stem cells, COLO-741, HCT-116, apoptosis, inflammation, acteoside.

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