The Importance of Expression of Keratin 17 (KRT17) and SPINK1 in Neoplastic (Invasive and Noninvasive) Lesions of the Bladder

Year 2022, Volume: 32 Issue: 4, 425 - 432, 31.08.2022

Serdar Uğraş , İsmail Harmankaya

https://doi.org/10.54005/geneltip.1119449

Abstract

Amaç: Mesane kanserlerinin tanı ve derecelendirilmesi tedavi ve prognoz üzerinde önemli bir etkiye sahiptir. Ancak günümüzde mesanenin neoplastik lezyonlarının ayırıcı tanısında kullanılabilecek çok hassas ve spesifik immünohistokimyasal paneller yoktur ve histomorfolojik bulgular halen altın standart olarak kabul edilmektedir. SPINK1, Keratin 17 (KRT17) ve Laminin immün boyalarının neoplastik mesane lezyonlarını ayırt etmedeki potansiyel önemini göstermeyi amaçladık.
Gereç ve Yöntem: KRT17, SPINK1 ve Laminin ekspresyonları immünohistokimyal yöntemle, toplam 141 doku örneğinde, neoplastik olmayan mesane mukozası (NBM) ve neoplastik mesane lezyonlarında araştırıldı.
Bulgular: KRT17 ve SPINK1 sıklıkla tümör dokularında eksprese edilir (sırasıyla %86,2 ve %68,7). NBM ve yedi neoplastik grubun tümü arasında KRT17 immün boyamasında istatistiksel olarak anlamlı bir fark tespit edildi (p=0.03 ila p0.001). NBM'de SPINK1 ekspresyonu, neoplazmlara kıyasla önemli ölçüde daha düşüktü. KRT17 ile tümör dokusundaki hücrelerin %2,5 veya daha fazlasının boyanması, neoplastik lezyonları neoplastik olmayan lezyonlardan, %86,3 duyarlılık ve %100 özgüllük ile ayırır. Bununla birlikte, tümör dokusundaki hücrelerin %12,5 veya daha fazlasının SPINK1 ile boyanması, neoplastik lezyonları neoplastik olmayan lezyonlardan, %62.6 duyarlılık ve %60 özgüllük ile ayırır. Neoplazmların %60'ında hem KRT17 hem de SPINK1 boyanmış olmasına rağmen, neoplastik hastaların %5,3'ünde ne KRT17 ne de SPINK1 boyanması görülmedi.
Sonuç: KRT17, SPINK1 ve Laminin'den oluşan immünohistokimyasal panel, mesane neoplazisinin doğru tanısında morfolojik bulgularla birlikte kullanılabilir.

Keywords

Bladder, Cancer, Immunohistochemistry, KRT17, SPINK1, TAT1

Supporting Institution

This study was funded by the Selçuk University Scientific Research Endorsement

Project Number

19401091

Thanks

We thank Dr. Serra Akar for the English corrections and Dr. Ömer Acat for the statistical study

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