Klebsiella pneumoniae Infection in Alveolar Type II Epithelial Cells; Antibiotic-Vitamin Combination Therapeutic Effect

Year 2023, Volume: 33 Issue: 5, 503 - 508, 25.10.2023

Özgür Çelebi , Demet Çelebi , Sümeyye Başer , Serkan Yıldırım , Mustafa Can Güler , Ali Taghizadehghalehjoughi

https://doi.org/10.54005/geneltip.1292360

Abstract

Aim: In this study, it was aimed to establish an infection model with Klebsiella pneumoniae on A549 Lung cancer cell line and to evaluate the effect of additional vitamins on the antibacterial effect of ampicillin sulbactam in the treatment. Cell culture and pathology results were determined in parallel with microbial analysis.
Methods: Minimal inhibitory concentration (MIC), fractional inhibitory concentration (FIC), biofilm optical density of ampicillin sulbactam, vitamins E, K1 and P on Klebicella pneumoniae ATCC 700603 strain were determined. Cytotoxic activity on A549 cancer cell line in parallel with microbial analysis and pathology results were determined.
Results: Ampicillin Sulbactam MIC dose range used in the treatment of Klebsiella pneumoniae infection is 16 mg/L. Looking at the FIC dose ranges, Vitamin K1+Ampicillin sulbactam, Vitamin P+Ampicillin sulbactam 2.5mg/ml+0.5µg/ml, Vitamin E+Ampicillin sulbactam 5mg/ml+0.5µg/ml, K1+P+E+Ampicillin sulbactam 2,5mg/ml+2.5mg/ml+5mg/ml+0.5µg/ml it was determined that it was. It was determined that it has a synergistic effect in combination with vitamins K1 and P antibiotics. In addition, in the presence of biofilm below 570 nm, the OD values of K1, P, E and K1+P+E+ antibiotic combinations were found to be 1.006, 0.969, 1.096 and 1.015, respectively. As a result of cell culture studies and evaluation of pathology results, it was determined that increasing the dose of Ampicillin sulbactam to 8 µg/ml increased the antibacterial effect and the vitamin combination was more effective.
Conclusions: Antibiotic and vitamin combination was found to be more effective in Klebsiella pneumoniae infection in our study. This situation contributes to the search for alternative antimicrobials.

Keywords

Lung cancer, Antibacterial activity, Antibiotic resistance, Biofilm, Synergistic effect, Vitamin

Alveolar Tip II Epitel Hücrelerinde Klebsiella pneumoniae Enfeksiyonu; Antibiyotik-Vitamin Kombinasyonunun Terapötik Etkisi

Abstract

Amaç: Bu çalışmada A549 Akciğer kanseri hücre hattı üzerinde Klebsiella pneumoniae ile enfeksiyon modeli oluşturup tedavide ampisilin sülbaktam antibakteriyel etkisine ek vitaminlerin etkisini değerlendirmesi amaçlandı. Mikrobiyal analiz ile paralel olarak hücre kültürü ve patoloji sonuçları belirlendi.
Yöntemler: Klebisella pneumoniae ATCC 700603 suşu üzerinde ampisilin sülbaktam, E, K1 ve P vitamininin minimal inhibitör konsantrasyonu (MİK), franksiyonel inhibitör konsantrasyonu (FİK), etkinlik gösterdiği biyofilm optik dansitesi belirlendi.Mikrobiyal analiz ile paralel olarak A549 kanser hücre hattı üzerinde sitotoksik aktivitesi ve patoloji sonuçları belirlendi.
Bulgular: Klebsiella pneumoniae enfeksiyonunun tedavisinde kullanılan Ampisilin Sulbaktam MİK doz aralığı 16 mg/L'dir. FİK doz aralıklarına bakıldığında Vitamin K1+Ampisilin sülbaktam, Vitamin P+Ampisilin sülbaktam 2.5mg/ml+0.5µg/ml, Vitamin E+Ampisilin sülbaktam 5mg/ml+0,5µg/ml, K1+P+E+Ampisilin sülbaktam 2,5mg/ml+2,5mg/ml+5mg/ml+0,5µg/ml olduğu belirlendi. Vitamin K1 ve P antibiyotik ile kombinasyon halinde sinerjistik etki gösterdiği belirlendi. Ayrıca 570 nm altında biyofilm varlığında K1,P,E ve K1+P+E+ antibiyotik kombinasyonlarının OD değerleri sırasıyla 1.006, 0.969, 1.096 ve 1.015 olarak bulundu. Hücre kültürü çalışmaları ve patoloji sonuçlarının değerlendirilmesi sonucunda Ampisilin sülbaktam dozunun 8 µg/ml'ye çıkarılması antibakteriyel etkiyi arttırdığı ve vitamin kombinasyonun daha etkili olduğu belirlendi.
Sonuç: Çalışmamızda oluşturulan Klebsiella pneumoniae enfeksiyonunda antibiyotik ve vitamin kombinasyonun daha etkili olduğu bulundu. Bu durum alternatif antimikrobiyal arayışlarına katkı sağlamaktadır.

Keywords

Akciğer kanseri, Antibakteriyel aktivite, Antibiyotik direnci, Biyofilm, Sinerjistik etki, Vitamin

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