Künt Toraks Travmasında Trimetilamin N-Oksit (TMAO) Düzeylerinin Değerlendirilmesi: Deneysel Bir Çalışma

Year 2024, Volume: 34 Issue: 3, 327 - 331, 30.06.2024

Fatma Şengül , Fikret Akyürek , Bahadir Ozturk , Hüsamettin Vatansev , Aysegül Bayır , Hasan Kara , Muslu Kazım Körez

https://doi.org/10.54005/geneltip.1407693

Abstract

Arka plan/Amaç: Toraks travmaları akciğer kontüzyonundan multi-organ yaralanmalarına kadar yaşamı tehdit eden problemlere neden olabilirken, akut akciğer yaralanması (ALI) ve akut solunum sıkıntısı sendromu (ARDS), travmatik yaralanmaların yaygın komplikasyonlarıdır. Künt toraks travmasının şiddetini belirlemek ve inflamatuar süreci takip etmek için biyokimyasal bir belirteç oluşturmak önemlidir. Bu çalışmada, tavşanlarda düşük, orta ve yüksek enerjili künt toraks travması modelinde serum Trimetilamin N-oksit (TMAO) düzeylerini ölçmek ve bu metabolitin tanısal değerini araştırmak amaçlanmıştır.
Gereç ve Yöntemler: Bu çalışmada, 27 Yeni Zelanda tavşanı dört gruba ayrılmıştır [kontrol, düşük enerjili travma grubu, orta enerjili travma grubu ve yüksek enerjili travma grubu]. Kan örnekleri toraks travmasından sonraki 1., 12. ve 24. saatlerde alınmıştır.
Bulgular: TMAO için hem gruplar içinde hem de gruplar arasında istatistiksel olarak anlamlı bir fark bulunmuştur (p<0.0001).
Sonuç: TMAO seviyeleri özellikle travmayı takip eden ilk saatte yükselmekte, 12 ve 24. Saatlerde ilk ate kıyasla düşmektedir (orta ve yüksek enerjili travma gruplarında). Bu bilgi potansiyel olarak travmanın hem şiddetinin hem de zamanlamasının bir göstergesi olabilir. Farklı enerji seviyeleri uygulanarak oluşturulan künt torasik travmaya bağlı ALI’de, TMAO seviyeleri gruplar arasında değişiklik göstermiş ve travmanın hem zamanlaması hem de şiddetiyle ilişkilendirilmiştir. Bu bulgular, TMAO düzeylerinin travmanın prognozunu değerlendirmede ve inflamatuar süreci izlemede değerli olabileceği önerisini desteklemektedir.

Keywords

Akut akciğer hasarı, Trimetilamin N-oksit, Biyobelirteç, Travma

Ethical Statement

Çalışma Helsinki Bildirgesi'ne uygun olarak yürütülmüş ve Selçuk Üniversitesi Deneysel Tıp Araştırma ve Uygulama Merkezi Etik Kurulu tarafından onaylanmıştır (protokol kodu 2022-39 ve onay tarihi 30/09/2022).

Evaluation of Trimethylamine N-Oxide (TMAO) Levels in Blunt Thoracic Trauma: An Experimental Study

Abstract

Background/Aims: Thoracic traumas cause life-threatening problems ranging from lung contusion to multiorgan injuries, while acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are common complications of traumatic injuries. It is important to establish a biochemical marker to determine the severity of blunt thoracic trauma and to follow the inflammatory process. To measure serum Trimethylamine N-oxide (TMAO) levels and to investigate the diagnostic value of this metabolite in a low, medium, and high energy blunt thoracic trauma model in rabbits.
Material and methods: In this study, 27 New Zealand rabbits were divided four groups [control, low energy trauma group, medium energy trauma group, and high energy trauma group]. Blood samples were obtained at 1st, 12th and 24th hour after thoracic trauma.
Results: There has been a statistically significant difference found both within groups and between groups for TMAO (p<0.0001).
Conclusions: TMAO levels are particularly elevated in the first hour following trauma, decreasing at 12 and 24 hours compared to the first hour (in the medium and high energy trauma groups). This information could potentially serve as an indicator of both the severity and timing of trauma. In blunt thoracic trauma-induced ALI created by applying different energy levels, TMAO levels varied among groups and were associated with both the timing and severity of the trauma. These findings support the suggestion that TMAO levels could be valuable in assessing the prognosis of trauma and monitoring the inflammatory process.

Keywords

Acute lung injury, Trimethylamine N-oxide, Biomarker, Trauma

Ethical Statement

The study was conducted in accordance with the Declaration of Helsinki and approved by the Ethics Committee of the University of Selçuk, Experimental Medicine Applications and Research Centre (protocol code 2022-39 and date of approval 30/09/2022) for studies.

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