ULTRASON DESTEKLİ ELMA ATIK ÖZÜTLERİNİN BAZI BİYOAKTİF ÖZELLİKLERİ

Year 2023, , 972 - 980, 15.10.2023

Hülya Şen Arslan

https://doi.org/10.15237/gida.GD23070

Abstract

Araştırmada elma atıklarının (yaprak, kabuk, posa) ultrason destekli su banyosunda 40 °C, %50 (333W) güç ve 2 saat sürede metanol ile alınan özütlerinin toplam fenolik madde miktarı (TFMM), antioksidan akivitesi, E. faecalis ve S. mutans inhibisyonu ve kolon kanser hücresi üzerine etkisi araştırılmıştır. Özütler içinde TFMM bakımından en yüksek değer yaprağa (104.72 mg/g) aittir. Atıkların antioksidan aktivitesi hem DPPH hem de ABTS yöntemiyle değerlendirilmiş ve her iki yöntemde de özütlerin yüksek antioksidan aktiviteye sahip olduğu bulunmuştur. Özütlerin patojenler üzerine antimikrobiyal aktivitesi olduğu bulunmuştur. Ayrıca; yaprak ve kabuk özütlerinin E. faecalis’e karşı posaya göre daha fazla inhibisyon potansiyeli olduğu görülmüştür. S. mutans üzerine en yüksek etkiyi ise yaprak özütleri göstermiştir. Çalışmada kullanılan kolon kanser (HT-29) hücresini özütlerin baskıladığı sonucuna varılmıştır. Sonuçlar, elma atıklarından ultrason ile alınan özütlerin patojen mikroorganizmalar ve kolon kanser hücresi üzerine inhibisyon aktivitesini ortaya koymuştur.

Keywords

antikanserojen, fenolik, kabuk, yaprak, posa

SOME BIOACTIVE PROPERTIES OF ULTRASOUND SUPPORTED APPLE WASTE EXTRACTS

Abstract

In the study, extracts of apple waste were taken in an ultrasound assisted water bath at 40 °C, 50% (333W) power and 2 hours with methanol. The total phenolic content (TFMM) and antioxidant activity of these extracts; E. faecalis and S. mutans inhibition and its effect on colon cancer were investigated. Among the extracts, the highest value in terms of TFMM belongs to the leaf (104.72 mg/g). The antioxidant activity of the wastes was evaluated by both DPPH and ABTS methods, and it was found that the extracts had high antioxidant activity in both methods. The extracts were found to have antimicrobial activity on pathogens. Moreover; It was observed that leaf and peel extracts had more inhibition potential against E. faecalis than pulp. Leaf extracts showed the highest effect on S. mutans. It was concluded that the extracts used in the study suppressed colon cancer (HT-29) cells.

Keywords

anticancer, phenolic, peel, pulp, leaf

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