KURU ÜZÜM TÜKETİMİNİN KUERSETİN BİYOYARARLANIMI ÜZERİNDEKİ ETKİSİ: IN VIVO YAKLAŞIM

Year 2024, Volume: 49 Issue: 4, 741 - 750, 14.08.2024

Ebru Aydın

https://doi.org/10.15237/gida.GD24049

Abstract

Kuru üzüm yüksek oranda diyet lifi, antioksidan ve ağırlıklı olarak kuersetin glikozitleri formunda bulunan kuersetin de dahil olmak üzere farklı biyoaktif bileşikler içermektedir. İçerdiği bu bileşiklerden dolayı sağlık üzerine yararlı etkileri olduğu bilinmektedir. Bu çalışmada, kuru üzüm tüketimini takiben idrardaki kuersetin atılımı analiz edilerek diyet matrisinin kuersetinin biyoyararlanımı üzerindeki etkisi araştırılmıştır. Çalışmada gelişmiş LC/MS teknikleri kullanılarak, diyetteki gıdaların, polifenol emilimine ve metabolizmasına etki derecesini tespit etmek için idrardaki kuersetin ve metabolitlerinin miktarları belirlemiştir. Kuru üzüm tükettikten sonra idrarla kuersetin atılımında önemli bir artış olduğunu ve konsantrasyonların 21.8 µg/ml ile 238.8 µg/ml arasında değiştiğini göstermiştir. Çalışma, kuersetinin biyoyararlanımını arttırmada gıda matrisinin rolünün önemini belirterek, kuru üzüm gibi işlenmemiş gıdalardaki karmaşık etkileşimlerin, kuersetinin çözünürlüğünü, stabilitesini ve emilimini önemli ölçüde etkileyebileceğini göstermektedir.

Keywords

Kuru üzüm, kuersetin, biyoyararlanım, atılım, besin matrisi

Ethical Statement

University of Leeds, MEEC Faculty Research Ethics Committee reference number MEEC 09-019.

Supporting Institution

This work is supported by Ministry of Turkish Education.

Thanks

This publication is part of the MSc thesis of Ebru Aydin, conducted under the supervision of Professor Gary Williamson at the University of Leeds. I acknowledge Professor Williamson for granting permission to publish this article without his name as an author. A consent letter has been provided and submitted to the journal, indicating that this article is published with his consent. The author would like to express gratitude to Professor Williamson and his team for their invaluable support and the resources they provided, which significantly contributed to this research.

THE IMPACT OF RAISIN CONSUMPTION ON QUERCETIN BIOAVAILABILITY: AN IN VIVO APPROACH IN VIVO

Abstract

Raisins are a nutrient-dense food known for their high content of dietary fiber, antioxidants, and bioactive compounds, including quercetin, which exists predominantly in the form of quercetin glycosides, enhancing their potential health benefits. This study investigates the impact of dietary matrix on quercetin bioavailability by analyzing urinary excretion following consumption of raisins. Employing advanced LC/MS techniques, the study quantified quercetin and its metabolites to evaluate how whole foods influence the absorption and metabolic processing of dietary polyphenols. Initial results indicated a significant increase in urinary quercetin excretion, with concentrations ranging from 21.8 µg/ml to 238.8 µg/ml among participants after consuming raisins. The study showed the role of the food matrix in enhancing quercetin bioavailability, suggesting that the complex interactions within whole foods like raisins could significantly influence the solubility, stability, and absorption of quercetin.

Keywords

Keywords: Raisins, quercetin, bioavailability, excretion, food matrix

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