Karanlıkla Teşvik Edilen Yaprak Senesensi Sürecinde Gibberellik Asit ve 6-Benzilaminopürinin Bazı Biyokimyasal Parametreler Üzerine Etkisi

Year 2016, , 17 - 24, 16.05.2016

Yener Okatan İlhami Karataş , Lokman Öztürk

https://doi.org/10.13002/jafag911

Abstract

Bu çalışmada uzun süre karanlık ortamda bekletilerek senesens süreci uyarılan Tropaeolum majus L. (Latin çiçeği) yapraklarına, gibberellik asit (GA3) ve 6-benzilaminopürin (BAP) uygulamanın bazı senesens parametrelerine etkisi incelenmiştir. Bu amaçla 10-5 M BAP ve 10-5 M GA3 uygulanarak karanlık ortama bırakılan yaprakların klorofil, protein, nişasta ve hidrojen peroksit miktarları ile katalaz ve peroksidaz enzimlerinin aktiviteleri belirlenmiştir. Bu iki hormon yaprakların klorofil ve protein içeriğinde meydana gelen kaybı azaltırken nişasta içeriğindeki düşüşü engelleyememiştir. BAP ve GA3 katalaz aktivitesini kontrole göre anlamlı şekilde artırırken peroksidaz aktivitesini azaltmıştır. Hidrojen peroksit içeriği BAP ve GA3 uygulanan yapraklarda belirgin şekilde azalmıştır. Bu sonuçlar, BAP ve GA3 uygulamanın yaprak senesensinin biyokimyasal parametrelerini değiştirerek bu sürecin geciktirilmesinde etkili olduğunu göstermektedir.

Keywords

Antioksidan enzimler, Benzilaminopürin, Gibberellik asit, Hidrojen peroksit, Senesens.

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