Comparison of Genome Sizes of Persimmon (Diospyros kaki L.) and Caucasian Persimmon (Diospyros lotus L.) Seedling Populations by Using Flow Cytometry

Year 2018, , 286 - 289, 31.12.2018

Murat Şeker Engin Gür Neslihan Ekinci Mehmet Ali Gündoğdu

https://doi.org/10.13002/jafag4502

Abstract

Flow cytometry is an improved technique for following cell cycle, determination of ploidy levels, calculation of genome sizes and early selection of candidates in plant breeding studies. Persimmon is generally propagated by using Diospyros lotus seedlings in Turkey. Moreover some persimmon varieties can be used for propagation as rootstock seed sources. In this study, fresh leaf tissues of Diospyros kaki and Diospyros lotus seedlings were used for obtaining of cell nuclei then stained by propidium iodide and were used for estimation of floresence intensities. According to the obtained results caucasian persimmon seedlings were had smaller genome sizes compare to the persimmon genomes and had less variation than persimmons. The relative ploidy level of caucasian persimmon seedlings were diploid where as hexploids in persimmon seedlings. By this experiment it was emphasized that new genotypes which have uniform rootstock charateristics for PCNA (Pollination Constant Non Astringent) and PVNA (Pollination Variant Non Astringent) persimmon varieties are needed for better performance in persimmon growing.

Keywords

Diospyros, rootstock, breeding, early selection, polyploidy, variation

References

• Arumuganathan K and Earle ED (1991). Estimation of nuclear DNA content of plants by flow cytometry. Plant Molecular Biology Reporter, 9 (3): 229-233.
• Choi Y, Tao R, Yonemori K and Sugiura A (2003). Genomic distribution of three repetitive DNAs in cultivated hexaploid Diospyros spp. (D. kaki and D. virginiana) and their wild relatives. Genes Genetic Systems, 78(4):301-8.
• Dolezel J, Dolezelova M and Novak FJ (1994). Flow cytometric estimation of nuclear DNA amount in diploid bananas (Musa acuminata and M. balbisiana). Biologia Plantarum, 36: 351- 357.
• Giordani E (2002). Varietal assortment of persimmon in the countries of the Mediterranean area and genetic improvement. First Mediterranean Symposium on Persimmon, University of Florence, 23-24 November 2001, p. 23-37. Faenza – Italy.
• Seker M (2001a). Determination of ploidy level and genome size by flow cytometry in cytological studies. Proceedings of the 1st Eurasian Congress on Molecular Biotechnology, s. 257–260. Trabzon, Turkey.
• Seker M (2001b). Estimation and comparison of nuclear DNA content of the seedling populations of important citrus rootstocks by flow cytometry. Proceedings of the 1st Eurasian Congress on Molecular Biotechnology, s. 261–264. Trabzon, Turkey.
• Seker M, Tuzcu O and Ollitrault P (2003). Comparison of nuclear DNA content of citrus rootstock populations by flow cytometry. Plant Breeding, 122(2): 169-172.
• Şeker M (2009). Investigation of isozyme polymorphism and nuclear DNA content variation of free–pollinated peach (Prunus persica L.) seedlings. Acta Horticulturae, 962: 177-183.