Immobilization of GOx on Trp / Trp-Fc Functionalized Nanospheres: Improved of Reusability and Stability

Year 2017, Volume: 30 Issue: 4, 114 - 122, 11.12.2017

Nurdan Kurnaz Yetim , Elvan Hasanoğlu Özkan , Nurşen Sarı

Abstract

In the study, new nanospheres support
containing ferrocene were synthesized and characterized, then the properties
of nanospheres as biocatalysis were researched. Novel support was prepared
from (Aminomethyl)polystyrene (AMP), tryptophan (Trp) and ferrocene aldehyde (Fc)  and the
enzymatic properties of immobilized 
glucose oxidase enzyme (GOx) was investigated. Modified
polystyrene was characterized IR spectra, Gel permeation chromatography and
Scanning electron microscopy. Ferrocene-tagged nanospheres-GOx
shows high reusability and storage capacity and fast incubation time
determination of glucose. After 12 months, immobilized GOx to the AMP-Trp
and AMP-Trp-Fc retained ca 20 % and
40 % for their original activity, respectively. High activities were found for
modified polymers AMP-Trp-Fc retains more than 35 % of the
initial activity after fifteenth successive cycles, which is a perfect performance.

Keywords

Biocatalysis, ferrocene, glucose oxidase, nanospheres

References

• Li, J., Qin, X., Yang, Z., Qi, H., Xu, Q., Diao, G., “A novel mesoporous silica nanosphere matrix for the immobilization of proteins and their applications as electrochemical biosensor”, Talanta, 104: 116-121 (2013).
• Torchilin, V.P., “Targeted pharmaceutical nanocarriers for cancer therapy and imaging”, AAPS J., 9:128-147 (2007).
• Wang, Y., Sun, H., Ang, H. M., Tade, M. O., Wang, S., “Synthesis of magnetic core/shell carbon nanosphere supported manganese catalysts for oxidation of organics in water by peroxymonosulfate”, J. Colloidal Interface Sci., 433: 68-75 (2014).
• Ahmad, R., Sardar, M., “Enzyme Immobilization: An Overview on nanoparticles as immobilization matrix”, Biochem. Anal. Biochem., 4: 1-8 (2015).
• Gupta, M. N., Kaloti, M., Kapoor, M., Solanki, K., “Nanomaterials as matrices for enzyme immobilization”, Artif. Cells Blood Substit. Immobil. Biotechnol., 39: 98-109 (2011).
• Ansari, S. A., Husain, Q., “Potential applications of enzymes immobilized on/in nano materials: A review”, Biotechnol. Adv., 30: 512-523 (2012).
• Verma, M. L., Barrow, C. J., Puri, M., “Nanobiotechnology as a novel paradigm for enzyme immobilisation and stabilisation with potential applications in biodiesel production”, Appl. Microbiol. Biotechnol. 97: 23-39 (2013).
• Hasanoğlu Özkan, E., Kurnaz Yetim, N., Nartop, D., Sarı, N., “Influence of load on the recycling stability of nanospheres attached platinium ion for determination of glucose”, Journal of Industrial and Engineering Chemistry, 25: 180-185 (2015).
• Min, K., Yoo, Y. J., “Recent progress in nanobiocatalysis for enzyme immobilization and its application”, Biotechnology and Bioprocess Engineering, 19: 553-567 (2014).
• Arslan, F., Beskan, U., “An amperometric biosensor for glucose detection from glucose oxidase immobilized in polyaniline-polyvinylsulfonate-potassium ferricyanide film”, Artif. Cells Nanomed. Biotechnol., 42(4): 284-288 (2014).
• Peng, H., Huang, Z., Zheng, Y., “A novel nanocomposite matrix based on graphene oxide and ferrocene-branched organically modified sol–gel/chitosan for biosensor application”, J. Solid State Chem., 18:1941-1949 (2014).
• Shim, J., Kim, G. Y., Moon, S. H., “Covalent co-immobilization of glucose oxidase and ferrocenedicarboxylic acid for an enzymatic biofuel cell”, J. Electroanal. Chem., 653:14-20 (2011).
• Yang,W., Zhou, H. Sun, “Synthesis of Ferrocene-Branched Chitosan Derivatives: Redox Polysaccharides and their Application to Reagentless Enzyme-Based Biosensors” C.Macromol.Papid.Commun., 28:265-270 (2007).
• Semashko, T., Mikhailova, R., Ramanaviciene, A., “Specificity of glucose oxidase from Penicillium funiculosum 46.1 towards some redox mediators” Appl. Biochem. Biotechnol., 171:1739-1749 (2013).
• Sung, D., Yang, S., “Facile method for constructing an effective electron transfermediating layer using ferrocene-containing multifunctional redoxcopolymer”, Electrochim. Acta, 133:40–48 (2014).
• Yetim, N. K., Özkan, E. H., Daniş, B., Tümtürk H., Sari, N., “Research on the Repeated Use of Novel Ferrocene- Tagged Nanomaterial for Determination of Glucose”, International Journal of Polymeric Materials and Polymeric Biomaterials, 64: 888-893, (2015).
• Trinder, P., “Determination of glucose in blood using glucose oxidase with on alternative oxygen receptor”, Ann. Clin. Biochem., 6:24-27 (1969).
• Sarı, N., Antepli, E., Nartop, D., Yetim, N. K., “Polystyrene attached Pt(IV)-azomethine, synthesis and immmobilization of glucose oxidase enzyme”, Int. J. Mol. Sci., 13:11870-11880 (2012).
• Popenoe, D. D., Deinhammer, R. S., Porter, M. D., ”Infrared spectroelectrochemical characterization of ferrocene-terminated alkanethiolate monolayers at gold”, Langmuir, 8:2521-2530 (1992).
• Özdem, N., Özkan, E. H., Sarı, N., Arslan, F., Tümtürk, H., “Immobilization of glucose oxidase attached to new nanospheres including azomethine” Macomol. Res., 22:1282- 1287 (2014).
• Arslan, F., Ustabas¸ S., Arslan, H., “An Amperometric Biosensor for Glucose Determination Prepared from Glucose Oxidase Immobilized in Polyaniline-Polyvinylsulfonate Film”, Sensors, 11:8152-8163 (2011).