Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane

Year 2018, Volume: 31 Issue: 4, 1022 - 1031, 01.12.2018

Naznoosh Shomalı–moghaddam Nursel Gul , Ebru Gokalp Ozkorkmaz , Aydin Ozluk

Abstract

Midgut cell and peritrophic membranes of   grain pest Agrotis segetum larvae
were examined by light microscope. Six biotinylated lectins were used in the
assays by applying histochemical methods (Avidin-Biotin-Peroxidase). The aim of
this study was to find lectins binding on peritrophic membranes if they were
available as insecticidal agents. Results of the study indicated that lectins
BPA (Bauhinia purpurea) and GS-I (Griffonia simplicifolia)
strongly stained whereas, PNA
(Pea Nut Agglutinin) and UEA-1 (Ulex europaeus) moderately stained the
tissues. However, WGA (Wheat Germ Agglutinin) and Con-A (Canavalia
ensiformis) stained the tissues weakly. The common feature of two binding proteins,
BPA and GS-I lectin was binding to D- galactose. Our examinations revealed
that   D-galactose mostly exists in A.
segetum larvae midgut cell
membranes and peritrophic membrane and this means BPA and GS-I lectins can be
used as insecticidal agents against A.
segetum.

Keywords

Agrotis segetum larvae, Biotinylated lectins, Midgut cells, Peritrophic membrane

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