Research Article
BibTex RIS Cite

Isolation, Identification and Phylogeny of Actinobacteria from Island Soils Using Different Isolation Methods

Year 2025, Early View, 1 - 1

Ahmet Rıdvan Topkara , Salih Sarıcaoğlu , Kamil Işık

https://doi.org/10.35378/gujs.1568577

Abstract

Actinobacteria are one of the most frequently studied prokaryotic groups within the Bacteria domain. In this study, soil samples collected from the islands of Burgazada, Büyükada, Gökçeada, Heybeliada, and Kınalıada were used to isolate, identify, and analyze the phylogeny of Actinobacteria. For the isolation studies, three different isolation methods and 11 different selective media were employed. As a result, a total of 103 bacterial strains were isolated. The molecular identification of the isolated strains was conducted using 16S rRNA gene region sequence analyses. These analyses revealed that the isolates belonged to 12 different genera within the Actinobacteria phylum. Comparison of the 16S rRNA gene sequences of the isolates with their closest relatives in the EzBioCloud database indicated a sequence similarity ranging between 95.76% and 100%. The isolation studies demonstrated that the standard dilution plate method was more effective for isolating both diverse genera and potential novel species. The data obtained through this method showed that 22 of the isolated strains, belonging to 8 different genera, have the potential to represent novel species. In conclusion, this study highlights that island soils are an important source for the discovery of new Actinobacteria species. Furthermore, it emphasizes the significance of such isolation studies in uncovering the rich biotechnological potential of Actinobacteria.

Keywords

Selective isolation 16S rRNA gene Molecular typing Actinobacterial diversity

Supporting Institution

Ondokuz Mayıs University

Project Number

PYO.FEN.1904.13.004

References

  • [1] Anandan, R., Dharumadurai, D., and Manogaran, G.P., “An introduction to actinobacteria, In Actinobacteria-basics and biotechnological applications”, IntechOpen, (2016).
  • [2] Boubekri, K., Soumare, A., Mardad, I., Lyamlouli, K., Ouhdouch, Y., Hafidi, M., and Kouisni, L., “Multifunctional role of Actinobacteria in agricultural production sustainability: A review”, Microbiological Research, 261: 127059, (2022). DOI: https://doi.org/10.1016/j.micres.2022.127059
  • [3] Whittaker R.J., Fernandez-Palacios J.M., ‘’Island Biogeography: Ecology, evolution, and conservation (2nd ed.)”, Oxford: Oxford University Press, (2007).
  • [4] Davison, J., Moora, M., Öpik, M., Ainsaar, L., Ducousso, M., Hiiesalu, I., and Zobel, M., “Microbial island biogeography: isolation shapes the life history characteristics but not diversity of root-symbiotic fungal communities”, International Society for Microbial Ecology Journal, 12(9): 2211-2224, (2018). DOI: https://doi.org/10.1038/s41396-018-0196-8
  • [5] Wang, P., Li, S.P., Yang, X., Zhou, J., Shu, W., and Jiang, L., “Mechanisms of soil bacterial and fungal community assembly differ among and within islands”, Environmental Microbiology, 22(4): 1559-1571, (2020). DOI: https://doi.org/10.1111/1462-2920.14864
  • [6] Lim, C.P., Hoon K.C., Cheah Y.K., “Actinobacteria from Greenwich Island and Dee Island: Isolation, diversity and distribution”, Life Sciences, Medicine and Biomedicine, 1-1, (2017). DOI: https://doi.org/10.28916/lsmb.1.1.2017.2
  • [7] Phongsopitanuna, W., Sripreechasakb P., Sangvichienc E., Tanasupawat S., “Diversity, antimicrobial activity, and susceptibility of culturable soil actinobacteria isolated from Sichang Island”, ScienceAsia, 47: 673-681, (2021). DOI: https://doi.org/10.2306/scienceasia1513-1874.2021.088
  • [8] Také, A., Inahashi, Y., Ōmura, S., Takahashi, Y., and Matsumoto, A., “Streptomyces boninensis sp. nov., isolated from soil from a limestone cave in the Ogasawara Islands”, International journal of systematic and evolutionary microbiology, 68(5): 1795-1799, (2018). DOI: https://doi.org/10.1099/ijsem.0.002753
  • [9] Kim, Y. S., Kim, S. B., “Agromyces seonyuensis sp. nov., isolated from island soil”, International Journal of Systematic and Evolutionary Microbiology, 73(1): 005689, (2023). DOI: https://doi.org/10.1099/ijsem.0.005689
  • [10] Jiang Y., Li Q., Chen X., and Jiang C., “Isolation and cultivation methods of Actinobacteria In Dhanasekaran D, Jiang Y. (editors)” Actinobacteria–Basics and Biotechnological Applications, 39–57, (2016).
  • [11] Yamamura H., Hayakawa M., and Limura Y., “Application of sucrose-gradient centrifugation for selective isolation of Nocardia sp. from soil”, Journal of Applied Microbiology, 95: 677–685, (2003). DOI: https://doi.org/10.1046/j.1365-2672.2003.02025.x
  • [12] Hayakawa, M., Yoshida, Y., and Iimura, Y., “Selective isolation of bioactive soil actinomycetes belonging to the Streptomyces violaceusniger phenotypic cluster”, Journal of Applied Microbiology, 96(5): 973-981, (2004). DOI: https://doi.org/10.1111/j.1365-2672.2004.02230.x
  • [13] Hayakawa, M., Nonomura, H., “Humic Acid-Vitamin Agar, A New Medium for The Selective Isolation of Soil Actinomycetes”, Journal of Fermentation Technology, 65: 501-509, (1987). DOI: https://doi.org/10.1016/0385-6380(87)90108-7
  • [14] Jones, K.L., ‘’Fresh Isolates of Actinomycetes in which The Presence of Sporogenous Aerial Mycelia is A Fluctuating Characteristic”, Journal of Bacteriology, 57: 141-145, (1949).
  • [15] Bower, C.S., Hucker, G.J., “Tech. Bull.” 228. New York State Agr. Exp. Sta., Geneva, N.Y., (1930).
  • [16] Shirling, E. B., Gottlieb, D., “Methods for Characterization of Streptomyces Species”, International Journal of Systematic Bacteriology, 16: 313-340, (1966). DOI: https://doi.org/10.1099/00207713-16-3-313
  • [17] Pridham T. G., Anderson P., Foley C., Lindenfelser H. A., Hesseltine C. W., and Benedict R. G., “A selection of media for maintenance and taxonomic study of Streptomyces”, Antibiotics Annual, 947-953, (1956/1957).
  • [18] Tan G. Y. A., Ward A. C., and Goodfellow M., “Exploration of Amycolatopsis diversity in soil using genus-specific primers and novel selective media”, Systematic and Applied Microbiology, 29: 557-569, (2006). DOI: https://doi.org/10.1016/j.syapm.2006.01.007
  • [19] Gordon R.E., Mihm J.M., ‘’The type species of the genus Nocardia”, Microbiology, 27: 1-10, (1962). DOI: https://doi.org/10.1099/00221287-27-1-1
  • [20] Kelly, K. L., ‘’Color-name charts illustrated with centroid colors. Inter-Society Color Council-National Bureau of Standards, Supplement to NBS Circ”, 533, Standard sample No. 2106, Chicagoİ, (1964).
  • [21] Saricaoglu, S., Isik, K., Veyisoglu, A., Saygin, H., Cetin, D., Guven, K., and Sahin, N., “Streptomyces burgazadensis sp. nov., isolated from soil”, International Journal of Systematic and Evolutionary Microbiology, 64(12): 4043-4048, (2014). DOI: https://doi.org/10.1099/ijs.0.065870-0
  • [22] Saricaoglu, S., Nouioui, I., Ay, H., Saygin, H., Bektas, K. I., Guven, K., Cetin, D., Klenk, H.P., Isik, k. and Sahin, N., ‘Nonomuraea insulae sp. nov., isolated from forest soil”, Antonie van Leeuwenhoek, 111: 2051-2059, (2018). DOI: https://doi.org/10.1007/s10482-018-1097-6
  • [23] Yoon S.H., Ha S.M., and Kwon S., ‘’Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies”, International Journal of Systematic and Evolutionary Microbiology, 67(5): 1613–1617, (2017). DOI: https://doi.org/10.1099/ijsem.0.001755
  • [24] Tamura K., Stecher G., and Kumar S., ‘’MEGA11: molecular evolutionary genetics analysis version 11”, Molecular Biology and Evolution, 38(7): 3022-3027, (2021). DOI: https://doi.org/10.1093/molbev/msab120
  • [25] Saitou, N., Nei, M., “The Neighbour-Joining Method: A New Method for Constructing Phylogenetic Trees”, Molecular and Biological Evolution, 4: 406-425, (1987). DOI: https://doi.org/10.1093/oxfordjournals.molbev.a040454
  • [26] Jukes, T.H., Cantor, C.R., “Evolution of protein molecules. In Mammalian Protein Metabolism”, 3, 21-132, Edited by H. N. Munro. New York: Academic Press, (1969).
  • [27] Chun, J., Goodfellow, M.A., “Phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences”, International Journal of Systematic and Evolutionary Microbiology, 45(2): 240-245, (1995). DOI: https://doi.org/10.1099/00207713-45-2-240
  • [28] Lane, D.J., “16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M. Nucleic acid techniques in bacterial systematics”, Wiley, New York, US, 115-175, (1991).
  • [29] Buchholz-Cleven, B.E.E., Rattunde, B., and Straub, K.L., ‘’Screening for genetic diversity of isolates of anaerobic Fe(II)-oxidizing bacteria using DGGE and whole-cell hybridization’’, Systematic and Applied Microbiology, 20(2): 301-309, (1997). DOI: https://doi.org/10.1016/S0723-2020(97)80077-X
  • [30] Stackebrandt, E., Goebel, B. M., “Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology”, International Journal of Systematic and Evolutionary Microbiology, 44(4): 846-849, (1994). DOI: https://doi.org/10.1099/00207713-44-4-846
  • [31] Chun, J., Oren, A., Ventosa, A., Christensen, H., Arahal, D. R., da Costa, M. S., and Trujillo, M. E., “Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes”, International Journal of Systematic and Evolutionary Microbiology, 68(1): 461-466, (2018). DOI: https://doi.org/10.1099/ijsem.0.002516
  • [32] Penkhrue, W., Sujarit, K., Kudo, T., Ohkuma, M., Masaki, K., Aizawa, T., Pathom-Aree, W., Khanongnuch, C., and Lumyong, S., ‘’Amycolatopsis oliviviridis sp. nov., a novel polylactic acidbioplastic-degrading actinomycete isolated from paddy soil’’, International Journal of Systematic and Evolutionary Microbiology, 68(5): 1448-1454, (2018). DOI: https://doi.org/10.1099/ijsem.0.002682
  • [33] Saricaoglu, S., Saygin, H., Topkara, A.R., Gencbay, T., Guven, K., Cetin, D., Sahin, N., and Isik, K., ‘’Nonomuraea basaltis sp. nov., a siderophore-producing actinobacteria isolated from surface soil of basaltic parent material’’, Archives of Microbiology, 202: 1535-1543, (2020). DOI: https://doi.org/10.1007/s00203-020-01866-3
  • [34] Songsumanus, A., Kuncharoen, N., Kudo, T., Yuki, M., Ohkuma, M., Igarashi, Y., and Tanasupawat, S., “Actinomadura decatromicini sp. nov., isolated from mountain soil in Thailand”, The Journal of Antibiotics, 74(1): 51-58, (2021). DOI: https://doi.org/10.1038/s41429-020-0353-y
  • [35] Hwang, Y. J., Son, J. S., Lee, S. Y., He, Y., Jo, Y., Shin, J. H., and Ghim, S. Y., “Nocardioides sambongensis sp. nov., isolated from Dokdo Islands soil”, International Journal of Systematic and Evolutionary Microbiology, 70(1): 16-22, (2020). DOI: https://doi.org/10.1099/ijsem.0.003689
  • [36] Kim, Y. S., Roh, S. G., and Kim, S. B., “Gordonia insulae sp. nov., isolated from an island soil”, International Journal of Systematic and Evolutionary Microbiology, 70(3): 2079-2083, (2020). DOI: https://doi.org/10.1099/ijsem.0.004023
  • [37] Tindall, B. J., Rosselló-Móra, R., Busse, H. J., Ludwig, W. and Kämpfer, P., “Notes on the characterization of prokaryote strains for taxonomic purposes”, The International Journal of Systematic and Evolutionary Microbiology, 60: 249-266, (2010). DOI: https://doi.org/10.1099/ijs.0.016949-0
  • [38] Nafis, A., Raklami, A., Bechtaoui, N., El Khalloufi, F., El Alaoui, A., Glick, B. R., and Hassani, L., “Actinobacteria from extreme niches in Morocco and their plant growth-promoting potentials”, Diversity, 11(8): 139, (2019). DOI: https://doi.org/10.3390/d11080139
  • [39] Nafis, A., Oubaha, B., Elhidar, N., Ortlieb, N., Kulik, A., Niedermeyer, T., and Barakate, M., “Novel production of two new nonpolyenic antifungal macrolide derivatives by Streptomyces Z26 isolated from Moroccan rhizospheric soil”, Online Journal of Biological Sciences, 18: 176-185, (2018). DOI: https://doi.org/10.3844/ojbsci.2018.176.185
  • [40] Biswas, K., Choudhury, J. D., Mahansaria, R., Saha, M., and Mukherjee, J., “Streptomyces euryhalinus sp. nov., a new actinomycete isolated from a mangrove forest”, The Journal of Antibiotics, 70(6): 747-753, (2017). DOI: https://doi.org/10.1038/ja.2017.3
  • [41] Tang, X., Zhao, J., Li, K., Chen, Z., Sun, Y., and Gao, J., “Streptomyces cyaneochromogenes sp. nov., a blue pigment-producing actinomycete from manganese-contaminated soil”, International Journal of Systematic and Evolutionary Microbiology, 69(8): 2202-2207, (2019). DOI: https://doi.org/10.1099/ijsem.0.003406

Year 2025, Early View, 1 - 1

Ahmet Rıdvan Topkara , Salih Sarıcaoğlu , Kamil Işık

https://doi.org/10.35378/gujs.1568577

Abstract

Project Number

PYO.FEN.1904.13.004

References

  • [1] Anandan, R., Dharumadurai, D., and Manogaran, G.P., “An introduction to actinobacteria, In Actinobacteria-basics and biotechnological applications”, IntechOpen, (2016).
  • [2] Boubekri, K., Soumare, A., Mardad, I., Lyamlouli, K., Ouhdouch, Y., Hafidi, M., and Kouisni, L., “Multifunctional role of Actinobacteria in agricultural production sustainability: A review”, Microbiological Research, 261: 127059, (2022). DOI: https://doi.org/10.1016/j.micres.2022.127059
  • [3] Whittaker R.J., Fernandez-Palacios J.M., ‘’Island Biogeography: Ecology, evolution, and conservation (2nd ed.)”, Oxford: Oxford University Press, (2007).
  • [4] Davison, J., Moora, M., Öpik, M., Ainsaar, L., Ducousso, M., Hiiesalu, I., and Zobel, M., “Microbial island biogeography: isolation shapes the life history characteristics but not diversity of root-symbiotic fungal communities”, International Society for Microbial Ecology Journal, 12(9): 2211-2224, (2018). DOI: https://doi.org/10.1038/s41396-018-0196-8
  • [5] Wang, P., Li, S.P., Yang, X., Zhou, J., Shu, W., and Jiang, L., “Mechanisms of soil bacterial and fungal community assembly differ among and within islands”, Environmental Microbiology, 22(4): 1559-1571, (2020). DOI: https://doi.org/10.1111/1462-2920.14864
  • [6] Lim, C.P., Hoon K.C., Cheah Y.K., “Actinobacteria from Greenwich Island and Dee Island: Isolation, diversity and distribution”, Life Sciences, Medicine and Biomedicine, 1-1, (2017). DOI: https://doi.org/10.28916/lsmb.1.1.2017.2
  • [7] Phongsopitanuna, W., Sripreechasakb P., Sangvichienc E., Tanasupawat S., “Diversity, antimicrobial activity, and susceptibility of culturable soil actinobacteria isolated from Sichang Island”, ScienceAsia, 47: 673-681, (2021). DOI: https://doi.org/10.2306/scienceasia1513-1874.2021.088
  • [8] Také, A., Inahashi, Y., Ōmura, S., Takahashi, Y., and Matsumoto, A., “Streptomyces boninensis sp. nov., isolated from soil from a limestone cave in the Ogasawara Islands”, International journal of systematic and evolutionary microbiology, 68(5): 1795-1799, (2018). DOI: https://doi.org/10.1099/ijsem.0.002753
  • [9] Kim, Y. S., Kim, S. B., “Agromyces seonyuensis sp. nov., isolated from island soil”, International Journal of Systematic and Evolutionary Microbiology, 73(1): 005689, (2023). DOI: https://doi.org/10.1099/ijsem.0.005689
  • [10] Jiang Y., Li Q., Chen X., and Jiang C., “Isolation and cultivation methods of Actinobacteria In Dhanasekaran D, Jiang Y. (editors)” Actinobacteria–Basics and Biotechnological Applications, 39–57, (2016).
  • [11] Yamamura H., Hayakawa M., and Limura Y., “Application of sucrose-gradient centrifugation for selective isolation of Nocardia sp. from soil”, Journal of Applied Microbiology, 95: 677–685, (2003). DOI: https://doi.org/10.1046/j.1365-2672.2003.02025.x
  • [12] Hayakawa, M., Yoshida, Y., and Iimura, Y., “Selective isolation of bioactive soil actinomycetes belonging to the Streptomyces violaceusniger phenotypic cluster”, Journal of Applied Microbiology, 96(5): 973-981, (2004). DOI: https://doi.org/10.1111/j.1365-2672.2004.02230.x
  • [13] Hayakawa, M., Nonomura, H., “Humic Acid-Vitamin Agar, A New Medium for The Selective Isolation of Soil Actinomycetes”, Journal of Fermentation Technology, 65: 501-509, (1987). DOI: https://doi.org/10.1016/0385-6380(87)90108-7
  • [14] Jones, K.L., ‘’Fresh Isolates of Actinomycetes in which The Presence of Sporogenous Aerial Mycelia is A Fluctuating Characteristic”, Journal of Bacteriology, 57: 141-145, (1949).
  • [15] Bower, C.S., Hucker, G.J., “Tech. Bull.” 228. New York State Agr. Exp. Sta., Geneva, N.Y., (1930).
  • [16] Shirling, E. B., Gottlieb, D., “Methods for Characterization of Streptomyces Species”, International Journal of Systematic Bacteriology, 16: 313-340, (1966). DOI: https://doi.org/10.1099/00207713-16-3-313
  • [17] Pridham T. G., Anderson P., Foley C., Lindenfelser H. A., Hesseltine C. W., and Benedict R. G., “A selection of media for maintenance and taxonomic study of Streptomyces”, Antibiotics Annual, 947-953, (1956/1957).
  • [18] Tan G. Y. A., Ward A. C., and Goodfellow M., “Exploration of Amycolatopsis diversity in soil using genus-specific primers and novel selective media”, Systematic and Applied Microbiology, 29: 557-569, (2006). DOI: https://doi.org/10.1016/j.syapm.2006.01.007
  • [19] Gordon R.E., Mihm J.M., ‘’The type species of the genus Nocardia”, Microbiology, 27: 1-10, (1962). DOI: https://doi.org/10.1099/00221287-27-1-1
  • [20] Kelly, K. L., ‘’Color-name charts illustrated with centroid colors. Inter-Society Color Council-National Bureau of Standards, Supplement to NBS Circ”, 533, Standard sample No. 2106, Chicagoİ, (1964).
  • [21] Saricaoglu, S., Isik, K., Veyisoglu, A., Saygin, H., Cetin, D., Guven, K., and Sahin, N., “Streptomyces burgazadensis sp. nov., isolated from soil”, International Journal of Systematic and Evolutionary Microbiology, 64(12): 4043-4048, (2014). DOI: https://doi.org/10.1099/ijs.0.065870-0
  • [22] Saricaoglu, S., Nouioui, I., Ay, H., Saygin, H., Bektas, K. I., Guven, K., Cetin, D., Klenk, H.P., Isik, k. and Sahin, N., ‘Nonomuraea insulae sp. nov., isolated from forest soil”, Antonie van Leeuwenhoek, 111: 2051-2059, (2018). DOI: https://doi.org/10.1007/s10482-018-1097-6
  • [23] Yoon S.H., Ha S.M., and Kwon S., ‘’Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies”, International Journal of Systematic and Evolutionary Microbiology, 67(5): 1613–1617, (2017). DOI: https://doi.org/10.1099/ijsem.0.001755
  • [24] Tamura K., Stecher G., and Kumar S., ‘’MEGA11: molecular evolutionary genetics analysis version 11”, Molecular Biology and Evolution, 38(7): 3022-3027, (2021). DOI: https://doi.org/10.1093/molbev/msab120
  • [25] Saitou, N., Nei, M., “The Neighbour-Joining Method: A New Method for Constructing Phylogenetic Trees”, Molecular and Biological Evolution, 4: 406-425, (1987). DOI: https://doi.org/10.1093/oxfordjournals.molbev.a040454
  • [26] Jukes, T.H., Cantor, C.R., “Evolution of protein molecules. In Mammalian Protein Metabolism”, 3, 21-132, Edited by H. N. Munro. New York: Academic Press, (1969).
  • [27] Chun, J., Goodfellow, M.A., “Phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences”, International Journal of Systematic and Evolutionary Microbiology, 45(2): 240-245, (1995). DOI: https://doi.org/10.1099/00207713-45-2-240
  • [28] Lane, D.J., “16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M. Nucleic acid techniques in bacterial systematics”, Wiley, New York, US, 115-175, (1991).
  • [29] Buchholz-Cleven, B.E.E., Rattunde, B., and Straub, K.L., ‘’Screening for genetic diversity of isolates of anaerobic Fe(II)-oxidizing bacteria using DGGE and whole-cell hybridization’’, Systematic and Applied Microbiology, 20(2): 301-309, (1997). DOI: https://doi.org/10.1016/S0723-2020(97)80077-X
  • [30] Stackebrandt, E., Goebel, B. M., “Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology”, International Journal of Systematic and Evolutionary Microbiology, 44(4): 846-849, (1994). DOI: https://doi.org/10.1099/00207713-44-4-846
  • [31] Chun, J., Oren, A., Ventosa, A., Christensen, H., Arahal, D. R., da Costa, M. S., and Trujillo, M. E., “Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes”, International Journal of Systematic and Evolutionary Microbiology, 68(1): 461-466, (2018). DOI: https://doi.org/10.1099/ijsem.0.002516
  • [32] Penkhrue, W., Sujarit, K., Kudo, T., Ohkuma, M., Masaki, K., Aizawa, T., Pathom-Aree, W., Khanongnuch, C., and Lumyong, S., ‘’Amycolatopsis oliviviridis sp. nov., a novel polylactic acidbioplastic-degrading actinomycete isolated from paddy soil’’, International Journal of Systematic and Evolutionary Microbiology, 68(5): 1448-1454, (2018). DOI: https://doi.org/10.1099/ijsem.0.002682
  • [33] Saricaoglu, S., Saygin, H., Topkara, A.R., Gencbay, T., Guven, K., Cetin, D., Sahin, N., and Isik, K., ‘’Nonomuraea basaltis sp. nov., a siderophore-producing actinobacteria isolated from surface soil of basaltic parent material’’, Archives of Microbiology, 202: 1535-1543, (2020). DOI: https://doi.org/10.1007/s00203-020-01866-3
  • [34] Songsumanus, A., Kuncharoen, N., Kudo, T., Yuki, M., Ohkuma, M., Igarashi, Y., and Tanasupawat, S., “Actinomadura decatromicini sp. nov., isolated from mountain soil in Thailand”, The Journal of Antibiotics, 74(1): 51-58, (2021). DOI: https://doi.org/10.1038/s41429-020-0353-y
  • [35] Hwang, Y. J., Son, J. S., Lee, S. Y., He, Y., Jo, Y., Shin, J. H., and Ghim, S. Y., “Nocardioides sambongensis sp. nov., isolated from Dokdo Islands soil”, International Journal of Systematic and Evolutionary Microbiology, 70(1): 16-22, (2020). DOI: https://doi.org/10.1099/ijsem.0.003689
  • [36] Kim, Y. S., Roh, S. G., and Kim, S. B., “Gordonia insulae sp. nov., isolated from an island soil”, International Journal of Systematic and Evolutionary Microbiology, 70(3): 2079-2083, (2020). DOI: https://doi.org/10.1099/ijsem.0.004023
  • [37] Tindall, B. J., Rosselló-Móra, R., Busse, H. J., Ludwig, W. and Kämpfer, P., “Notes on the characterization of prokaryote strains for taxonomic purposes”, The International Journal of Systematic and Evolutionary Microbiology, 60: 249-266, (2010). DOI: https://doi.org/10.1099/ijs.0.016949-0
  • [38] Nafis, A., Raklami, A., Bechtaoui, N., El Khalloufi, F., El Alaoui, A., Glick, B. R., and Hassani, L., “Actinobacteria from extreme niches in Morocco and their plant growth-promoting potentials”, Diversity, 11(8): 139, (2019). DOI: https://doi.org/10.3390/d11080139
  • [39] Nafis, A., Oubaha, B., Elhidar, N., Ortlieb, N., Kulik, A., Niedermeyer, T., and Barakate, M., “Novel production of two new nonpolyenic antifungal macrolide derivatives by Streptomyces Z26 isolated from Moroccan rhizospheric soil”, Online Journal of Biological Sciences, 18: 176-185, (2018). DOI: https://doi.org/10.3844/ojbsci.2018.176.185
  • [40] Biswas, K., Choudhury, J. D., Mahansaria, R., Saha, M., and Mukherjee, J., “Streptomyces euryhalinus sp. nov., a new actinomycete isolated from a mangrove forest”, The Journal of Antibiotics, 70(6): 747-753, (2017). DOI: https://doi.org/10.1038/ja.2017.3
  • [41] Tang, X., Zhao, J., Li, K., Chen, Z., Sun, Y., and Gao, J., “Streptomyces cyaneochromogenes sp. nov., a blue pigment-producing actinomycete from manganese-contaminated soil”, International Journal of Systematic and Evolutionary Microbiology, 69(8): 2202-2207, (2019). DOI: https://doi.org/10.1099/ijsem.0.003406
There are 41 citations in total.

Details

Primary Language English
Subjects Microbial Taxonomy, Bacteriology
Journal Section Research Article
Authors

Ahmet Rıdvan Topkara 0000-0001-5813-1095

Salih Sarıcaoğlu 0000-0002-0013-9024

Kamil Işık 0000-0003-1764-8113

Project Number PYO.FEN.1904.13.004
Early Pub Date February 4, 2025
Publication Date
Submission Date October 16, 2024
Acceptance Date December 28, 2024
Published in Issue Year 2025 Early View

Cite

APA Topkara, A. R., Sarıcaoğlu, S., & Işık, K. (2025). Isolation, Identification and Phylogeny of Actinobacteria from Island Soils Using Different Isolation Methods. Gazi University Journal of Science1-1. https://doi.org/10.35378/gujs.1568577
AMA Topkara AR, Sarıcaoğlu S, Işık K. Isolation, Identification and Phylogeny of Actinobacteria from Island Soils Using Different Isolation Methods. Gazi University Journal of Science. Published online February 1, 2025:1-1. doi:10.35378/gujs.1568577
Chicago Topkara, Ahmet Rıdvan, Salih Sarıcaoğlu, and Kamil Işık. “Isolation, Identification and Phylogeny of Actinobacteria from Island Soils Using Different Isolation Methods”. Gazi University Journal of Science, February (February 2025), 1-1. https://doi.org/10.35378/gujs.1568577.
EndNote Topkara AR, Sarıcaoğlu S, Işık K (February 1, 2025) Isolation, Identification and Phylogeny of Actinobacteria from Island Soils Using Different Isolation Methods. Gazi University Journal of Science 1–1.
IEEE A. R. Topkara, S. Sarıcaoğlu, and K. Işık, “Isolation, Identification and Phylogeny of Actinobacteria from Island Soils Using Different Isolation Methods”, Gazi University Journal of Science, pp. 1–1, February 2025, doi: 10.35378/gujs.1568577.
ISNAD Topkara, Ahmet Rıdvan et al. “Isolation, Identification and Phylogeny of Actinobacteria from Island Soils Using Different Isolation Methods”. Gazi University Journal of Science. February 2025. 1-1. https://doi.org/10.35378/gujs.1568577.
JAMA Topkara AR, Sarıcaoğlu S, Işık K. Isolation, Identification and Phylogeny of Actinobacteria from Island Soils Using Different Isolation Methods. Gazi University Journal of Science. 2025;:1–1.
MLA Topkara, Ahmet Rıdvan et al. “Isolation, Identification and Phylogeny of Actinobacteria from Island Soils Using Different Isolation Methods”. Gazi University Journal of Science, 2025, pp. 1-1, doi:10.35378/gujs.1568577.
Vancouver Topkara AR, Sarıcaoğlu S, Işık K. Isolation, Identification and Phylogeny of Actinobacteria from Island Soils Using Different Isolation Methods. Gazi University Journal of Science. 2025:1-.