Effects of Bacillus thuringiensis kurstaki on Midgut Cells of Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae), The Pine Processionary Caterpillar

Year 2014, Volume: 27 Issue: 2, 861 - 870, 27.06.2014

Meltem Uzunhisarcıklı , Yusuf Kalender , Zekiye Suludere

Abstract

In this study effects of Bacillus thuringiensis kurstaki (Btk) on midgut cells of Thaumetopoea pityocampa larvae was investigated by electron microscopy. 3 mg/l Btk was given with food. 1 h, 3 h and 6 h after Btk administration no pathological changes in the midgut epitelial cells were observed. 12 h after Btk administration swelling of mitochondria in midgut epitelial cells were observed. Formation of vacuoles in cytoplasm was seen after 24 h. After 48 h decreasing of microvilli of midgut epithelial cell’s and partial dissolving of cytoplasm, dissolving of nucleoplasm and chromatin clumps were observed. 60 h after Btk administration swelling and dissolving of mitochondria in midgut epithelial cells were detected. 96 h after Btk administration a membrane-body and dissolving in the cytoplasm clumps in midgut cells of T. pityocampa larvae were occurred. Present study shows that Btk is effective to T. pityocampa larvae.

Keywords

Thaumetopoea pityocampa, Bacillus thuringiensis kurstaki, midgut, ultrastructure, transmission electron microscope

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