Effects of Different Culture Media Compositions on In Vitro Micropropagation from Paradox Walnut Rootstock Nodes

Year 2022, Volume: 9 Issue: 4, 500 - 515, 31.12.2022

Cem Dirlik , Hacer Kandemir , Nurberat Çetin , Senem Şen , Begüm Güler , Aynur Gürel

https://doi.org/10.54287/gujsa.1194822

Cited By: 3

Abstract

The aim of this study is to establish an effective protocol for in vitro micropropagation from node explants of the 'Paradox' (Juglans regia x J. hindsii) rootstock. In the first stage of the study, the node explants were cultured in semi-solid media after being exposed to different sterilization methods. The most effective sterilization method was observed as ST2 with the lowest darkening rate (4 %). According to the results, SP4 medium provided the highest number of shoots per unit explant (1.05 shoots/explant) and 100 % shoot formation, SP2 medium provided the best mean shoot length (1.6 cm) and the mean number of nodes per unit explant (7.95 nodes/explant). Best root induction was provided from ½ Modified MS medium containing 4 mg/L IBA with 30 g/L sucrose. For root induction explants were retained under dark conditions for 7 days at 24±2oC. In order to ensure root formation and elongation, shoot explants with root induction cultured in a 16-hour photoperiod (3500 lux) for 21 days in different nutrient media containing 2.4 mg/L Gelrite, hormone-free and mixed with vermiculite (1:1 v/v) and was rooting rate is 45.45 % in ¼DFe medium.

Keywords

Micropropagation, Walnut, Juglans regia L., Paradox, In vitro

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