Investigation of Antimicrobial, Antioxidant, and Antimutagenic Activities of Wild Guelder RoseFruit Extract Grown in Ardahan and Its Surrounding Area

Year 2018, Volume: 8 Issue: 1, 18 - 25, 31.01.2018

Mehmet Arslan , Nurcan Erbil , Zehra tuğba Murathan

https://doi.org/10.17714/gumusfenbil.294719

Cited By: 3

Abstract

In this study, antioxidant activity, antibacterial effect against some
test bacteria, and antimutagenic effect with AMES test of Guelder rose fruit
naturally growing in Ardahan and its surrounding area were examined. Different
Gr (+), and Gr (-) bacteria and yeasts were used as test microorganisms during
the antibacterial activity tests. AMES / Salmonella/Microsome test was
preferred for antimutagenic effect study and Salmonella typhimurium TA 98 and
TA 100 strains were used. According to the obtained data, 0.80 g fruitweight on
average, 11.3% of Soluble Dry Matter content, 3.20 of pH, 0.80 mg/g of total
anthocyanin, and 52.5% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical
scavenging activity, 72.8 µmol TE/g FW 2,2′-Azino-bis
(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) 
value, 122.6 µmol Fe II/g FW Ferric Reducing Antioxidant Power (FRAP)
value were determined. It was determined that Guelder rose fruit extract has
antibacterial activity at different rates on test bacteria, but not antifungal
activity. The most sensitive bacterium was Pseudomonas aeroginosa ATCC 9027
among the test microorganisms. In antimutagenic activity, only 40 μl dose of
Guelder rose in TA98 strains caused a significant reduction compared to the
positive control. In TA100 strains, no statistically significant decrease compared
to positive control at any dose occurred.

Keywords

Antimicrobial, Antimutagenic, Antioxidant, Guelder rose, Viburnum opulus

Ardahan ve Çevresinde Yabani Olarak Yetişen Gilaburu Meyve Ekstraktının Antimikrobiyal, Antioksidan ve Antimutajenik Aktivitelerinin Araştırılması

Abstract

Bu çalışmada Ardahan ve çevresinde doğal olarak
yetişmekte olan Gilaburu meyvelerinin antioksidan özellikleri, farklı
mikroorganizmalar üzerindeki antimikrobiyal etkisi ve Ames testi ile
antimutajenik etkisi araştırıldı. Antimikrobiyal aktivite testleri esnasında
farklı Gr (+) ve Gr (-) bakteriler ile mayalar kullanıldı. Antimutajenik etki
çalışmalarında ise AMES/Salmonella Mikrozom testi ile Salmonella typhimurium TA
98 ve TA 100 suşları kullanıldı. Elde edilen verilere göre gilaburu
meyvelerinin ağırlığı ortalama olarak 0.80 g, suda çözünür kuru madde (SÇKM)
içeriği % 11.3, pH’sı 3.20, toplam antosiyanin miktarı 0.80
mg/g,1,1-diphenyl-2-picrylhydrazyl (DPPH)radikal süpürücü etkisi % 52.5,
2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) değeri 72.8 µmol
TE/g FW, Ferric Reducing Antioxidant Power (FRAP) değeri ise 122.6 µmol Fe II/g
FW olarak belirlendi. Ayrıca gilaburu ekstraktının test mikroorganizmaları
üzerinde değişen oranlarda antibakteriyel aktiviteye sahip oldukları; ancak
antifungal aktivite sergilemedikleri belirlendi. Test bakterileri içerisinde
ise en duyarlı olanının Pseudomonas aeruginosa ATCC 9027 olduğu belirlendi.
Antimutajenite deneylerinde,TA98 suşlarında gilaburunun sadece 40 µl dozunda
pozitif kontrole göre önemli bir azalma meydana getirdi. TA100 suşlarında ise
hiçbir dozda pozitif kontrole göre istatistiksel olarak önemli bir azalma
meydana gelmediği belirlendi.

Keywords

Antimikrobiyal, Antimutajen, Antioksidan, Gilaburu, Viburnum opulus

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