Research Article
Galanthus ikariae Baker Bitkisinin Toprak Üstü Kısımlarının Uçucu Yağının Kimyasal Bileşimi ve Biyolojik Aktiviteleri
Abstract
Bu
çalışmada Galanthus ikariae
Baker bitkisinin toprak üstü kısımlarının uçucu yağının kimyasal içeriği,
antioksidan ve antimikrobiyal özellikleri araştırılmıştır. Bitkinin uçucu yağı
Clevenger tipi hidrodistilasyon ile alınmış ve GC-MS/FID ile analiz edilmiştir.
Uçucu yağdaki 41 bileşiğin yapısı literatür bilgileri ile karşılaştırılarak
aydınlatılmıştır. Hegzadekanoik asit, linoleik asit, 2-tridekanon ve linoleik
asit metil ester bileşiklerinin sırasıyla %19.05; %14.6; %11.49 ve %6.97
oranlarında ana bileşenler oldukları tespit edilmiştir. Uçucu yağın toplam
fenolik miktarı ve radikal süpürücü aktivitesi (DPPH) sırasıyla 342.22 mg/mL GAE ve %55.92 bulunmuştur. Uçucu yağın
antimikrobiyal aktivitesi agar kuyucuk metodu ile 12 adet bakteri ve 5 adet maya-küfe
karşı araştırılmıştır ve özellikle Listeria monocytogenes bakterisi ve Saccharomyces cerevisiae mayasına karşı etkili olduğu
görülmüştür.
References
- Adams, R.P., 1997. Identification of essential oil components by gas chromatography/mass spectroscopy. Journal of the American Society for Mass Spectrometry 8(6), 671-672.
- Ay, B.E., Açikgöz, M.A., Yarilgaç, T. Ve Kara, M., 2018. Assessment of Antioxidant Activity of Giant Snowdrop (Galanthus elwesii Hook) Extracts with Their Total Phenol and Flavonoid Contents. Indian Journal of Pharmaceutical Education and Research 52(4) 128-132.
- Baytop, T., 1999. Bitkiler İle Tedavi (Geçmişte ve Bugün). İstanbul, Nobel Tıp Kitabevleri, 480 s.
- Brickell, C. D., 1984. Galanthus L. In: Flora of Turkey and the Est Aegean Islands, in: Davis, P.H. (Ed.), Vol. 8, Edinburgh University Press, Edin-Burgh ISBN: 9780852244944, pp. 365-372.
- Bokov, D.O., Samylina, I. A. ve Nikolov, S. D., 2016. Amaryllidaceae alkaloids GC/MS analysis in Galanthus woronowii and Galanthus nivalis of Russian origin. Research Journal of Pharmaceutical Biological and Chemical Sciences 7(6), 1625-1629.
- Bokov, D.O. ve Samylina, I.A., 2017. Comparison of the Chemical Compositions of Galanthus woronowii Losinsk. and Galanthus nivalis L. Homeopathic Mother Tinctures by Gas Chromatography with Mass-Selective Detection. Pharmaceutical Chemistry Journal 50(10), 659-667.
- Bozkurt, B., Emir, A., Kaya, G.İ., Onur, M.A., Berkov, S., Bastida, J. ve Unver Somer N., 2017. Alkaloid profiling of Galanthus woronowii Losinsk. by GC-MS and evaluation of its biological activity. Marmara Pharmaceutical Journal 21(4), 915-920.
- Emir, A., 2010. Galanthus woronowii Losinsk. Bitkisinin Kalite Kontrol Açısından Değerlendirilmesi, Yüksek Lisans Tezi, Ege Üniversitesi Sağlık Bilimleri Enstitüsü, İzmir, 147 s.
- Finley, J.W. ve Given Jr, P., 1986. Technological necessity of antioxidants in the food industry. Food and Chemical Toxicology, 24(10-11), 999-1006.
- Gámez-Meza, N., Noriega-Rodríguez, J.A., Medina-Juárez, L.A., Ortega-García, J., Cázarez-Casanova, R. ve Angulo-Guerrero, O., 1999. Antioxidant activity in soybean oil of extracts from Thompson grape bagasse. Journal of the American Oil Chemists' Society 76(12), 1445.
- Karimi, E., Mehrabanjoubani, P., Homayouni-Tabrizi, M., Abdolzadeh, A. ve Soltani, M., 2018. Phytochemical evaluation, antioxidant properties and antibacterial activity of Iranian medicinal herb Galanthus transcaucasicus Fomin. Journal of Food Measurement and Characterization, 12(1), 433-440.
- Kaya, G. İ., Kıvçak, B. ve Gözler, B., 2002. Galanthus nivalis L. subsp. cilicicus (Baker) Gottlieb-Tannenhain bitkisinden hazırlanan herba ve bulbus galanthi drogları üzerinde DAB 10'A göre yapılan kalite kontrol çalışmaları. 14. Bitkisel İlaç Hammaddeleri Toplantısı, 29-31 Mayıs 2002, Eskişehir, 199-202.
- Koyuncu, M., 1997. Türkiye’den İhraç Edilen Geofitlerin Korunması ve Üretimi Konusunda Gelişmeler, In: XI. Bitkisel İlaç Hammaddeleri Toplantısı Bildiri Kitabı (editörler: M. Kükcüoğlu, KHC. Baser), Ankara Üniversitesi Eczacılık Fakültesi, 57-62.
- Noyan, S., Rentsch, G.H., Önür, M.A., Gözler, T., Gözler, B., ve Hesse, M., 1998. The Gracilines: a novel subgroup of the Amaryllidaceae Alkaloids. Org-Chem.Inst., 29(51), 1777-1791.
- Pino, J. A., Mesa, J., Muñoz, Y., Martí, M. P. ve Marbot, R., 2005. Volatile components from mango (Mangifera indica L.) cultivars. Journal of Agricultural and Food Chemistry, 53(6), 2213-2223,
- Rainer, M., 1997. Galanthamine in Alzheimer’s disease. CNS Drugs, 7(2), 89-97.
- Saǧdıç, O. ve Özcan, M., 2003. Antibacterial activity of Turkish spice hydrosols. Food Control, 14(3), 141-143.
- Sarikaya, B.B., Kaya, G.I., Onur, M.A., Bastida, J. ve Somer, N.U., 2013. Phytochemical investigation of Galanthus woronowii. Biochemical Systematics and Ecology, 51, 276-279.
- Sener, K., Koyuncu, M., Bingöl, F. ve Muhtar V., 1999. Production of bioactive alkaloids from Turkish geophytes. IUPAC, 23–27 November 1997, Phuket, Thailand. 1-7.
- Ünver, N., Gözler, T., Walch, N., Gözler, B., ve Hesse, M., 1999. Two novel dinitrogenous alkaloids from Galanthus plicatus subsp. byzantinus (Amaryllidaceae). Phytochemistry, 50(7), 1255-1261.
- Zhao, C., Zeng, Y., Wan, M., Li, R., Liang, Y., Li, C., Zeng , Z. ve Chau, F.T., 2009. Comparative analysis of essential oils from eight herbal medicines with pungent flavor and cool nature by GC–MS and chemometric resolution methods. Journal of Separation Science, 32(4), 660-670.
Chemical Constituents and Biological Activities of Essential Oil from Aerial Parts of Galanthus ikariae Baker
Abstract
In this study, the chemical composition,
antioxidant and antimicrobial properties of the volatile oil of aerial parts Galanthus ikariae Baker plant were
investigated. The essential oil was
obtained by Clevenger type hydrodistillation and analysed by GC-MS/FID. The
structures of 41 compounds in essential oil were compared with the literature
information. Hexadecanoic acid, linoleic acid, 2-tridecanone and linoleic acid
methyl ester compounds were the main components and found as 19.05%; 14.67%;
11.49% and 6.97%, respectively. The amount of total phenolic and, %DPPH˙ scavenging activity were found as 342.22 mg/mL GAE and %55.92, respectively. The antimicrobial activity of
essential
oil was investigated against twelve bacteria and 5 fungi, by using agar
dilution method. Essential oil was effective against Listeria monocytogenes and Saccharomyces
cerevisiae, especially.
References
- Adams, R.P., 1997. Identification of essential oil components by gas chromatography/mass spectroscopy. Journal of the American Society for Mass Spectrometry 8(6), 671-672.
- Ay, B.E., Açikgöz, M.A., Yarilgaç, T. Ve Kara, M., 2018. Assessment of Antioxidant Activity of Giant Snowdrop (Galanthus elwesii Hook) Extracts with Their Total Phenol and Flavonoid Contents. Indian Journal of Pharmaceutical Education and Research 52(4) 128-132.
- Baytop, T., 1999. Bitkiler İle Tedavi (Geçmişte ve Bugün). İstanbul, Nobel Tıp Kitabevleri, 480 s.
- Brickell, C. D., 1984. Galanthus L. In: Flora of Turkey and the Est Aegean Islands, in: Davis, P.H. (Ed.), Vol. 8, Edinburgh University Press, Edin-Burgh ISBN: 9780852244944, pp. 365-372.
- Bokov, D.O., Samylina, I. A. ve Nikolov, S. D., 2016. Amaryllidaceae alkaloids GC/MS analysis in Galanthus woronowii and Galanthus nivalis of Russian origin. Research Journal of Pharmaceutical Biological and Chemical Sciences 7(6), 1625-1629.
- Bokov, D.O. ve Samylina, I.A., 2017. Comparison of the Chemical Compositions of Galanthus woronowii Losinsk. and Galanthus nivalis L. Homeopathic Mother Tinctures by Gas Chromatography with Mass-Selective Detection. Pharmaceutical Chemistry Journal 50(10), 659-667.
- Bozkurt, B., Emir, A., Kaya, G.İ., Onur, M.A., Berkov, S., Bastida, J. ve Unver Somer N., 2017. Alkaloid profiling of Galanthus woronowii Losinsk. by GC-MS and evaluation of its biological activity. Marmara Pharmaceutical Journal 21(4), 915-920.
- Emir, A., 2010. Galanthus woronowii Losinsk. Bitkisinin Kalite Kontrol Açısından Değerlendirilmesi, Yüksek Lisans Tezi, Ege Üniversitesi Sağlık Bilimleri Enstitüsü, İzmir, 147 s.
- Finley, J.W. ve Given Jr, P., 1986. Technological necessity of antioxidants in the food industry. Food and Chemical Toxicology, 24(10-11), 999-1006.
- Gámez-Meza, N., Noriega-Rodríguez, J.A., Medina-Juárez, L.A., Ortega-García, J., Cázarez-Casanova, R. ve Angulo-Guerrero, O., 1999. Antioxidant activity in soybean oil of extracts from Thompson grape bagasse. Journal of the American Oil Chemists' Society 76(12), 1445.
- Karimi, E., Mehrabanjoubani, P., Homayouni-Tabrizi, M., Abdolzadeh, A. ve Soltani, M., 2018. Phytochemical evaluation, antioxidant properties and antibacterial activity of Iranian medicinal herb Galanthus transcaucasicus Fomin. Journal of Food Measurement and Characterization, 12(1), 433-440.
- Kaya, G. İ., Kıvçak, B. ve Gözler, B., 2002. Galanthus nivalis L. subsp. cilicicus (Baker) Gottlieb-Tannenhain bitkisinden hazırlanan herba ve bulbus galanthi drogları üzerinde DAB 10'A göre yapılan kalite kontrol çalışmaları. 14. Bitkisel İlaç Hammaddeleri Toplantısı, 29-31 Mayıs 2002, Eskişehir, 199-202.
- Koyuncu, M., 1997. Türkiye’den İhraç Edilen Geofitlerin Korunması ve Üretimi Konusunda Gelişmeler, In: XI. Bitkisel İlaç Hammaddeleri Toplantısı Bildiri Kitabı (editörler: M. Kükcüoğlu, KHC. Baser), Ankara Üniversitesi Eczacılık Fakültesi, 57-62.
- Noyan, S., Rentsch, G.H., Önür, M.A., Gözler, T., Gözler, B., ve Hesse, M., 1998. The Gracilines: a novel subgroup of the Amaryllidaceae Alkaloids. Org-Chem.Inst., 29(51), 1777-1791.
- Pino, J. A., Mesa, J., Muñoz, Y., Martí, M. P. ve Marbot, R., 2005. Volatile components from mango (Mangifera indica L.) cultivars. Journal of Agricultural and Food Chemistry, 53(6), 2213-2223,
- Rainer, M., 1997. Galanthamine in Alzheimer’s disease. CNS Drugs, 7(2), 89-97.
- Saǧdıç, O. ve Özcan, M., 2003. Antibacterial activity of Turkish spice hydrosols. Food Control, 14(3), 141-143.
- Sarikaya, B.B., Kaya, G.I., Onur, M.A., Bastida, J. ve Somer, N.U., 2013. Phytochemical investigation of Galanthus woronowii. Biochemical Systematics and Ecology, 51, 276-279.
- Sener, K., Koyuncu, M., Bingöl, F. ve Muhtar V., 1999. Production of bioactive alkaloids from Turkish geophytes. IUPAC, 23–27 November 1997, Phuket, Thailand. 1-7.
- Ünver, N., Gözler, T., Walch, N., Gözler, B., ve Hesse, M., 1999. Two novel dinitrogenous alkaloids from Galanthus plicatus subsp. byzantinus (Amaryllidaceae). Phytochemistry, 50(7), 1255-1261.
- Zhao, C., Zeng, Y., Wan, M., Li, R., Liang, Y., Li, C., Zeng , Z. ve Chau, F.T., 2009. Comparative analysis of essential oils from eight herbal medicines with pungent flavor and cool nature by GC–MS and chemometric resolution methods. Journal of Separation Science, 32(4), 660-670.
There are 21 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Engineering |
| Journal Section | Articles |
| Authors | |
| Publication Date | October 15, 2019 |
| Submission Date | February 12, 2019 |
| Acceptance Date | June 21, 2019 |
| Published in Issue | Year 2019 Volume: 9 Issue: 4 |
