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Antimicrobial Effect of Traditional Breakfast Zahter

Year 2020, Volume: 10 Issue: 1, 128 - 133, 15.01.2020
https://doi.org/10.17714/gumusfenbil.579489

Abstract

Today, the active ingredients of the preparations used
in the treatment of some diseases can be completely herbal source. Some plants
or spice mixtures are known to have antiviral, antibacterial and antifungal
effects, and are known to be used as supplements to treat simple infections or
to strengthen the immune system as a preservative. The zahter used in the study
is a spice mixture that is consumed as breakfast in Southeastern Anatolia.

In this study; The antimicrobial effects of
zahterin Escherichia coli ATCC 25322, Pseudomonas aeruginosa DMS50071 SCOTTA,
Klebsiella pneumoniae ATCC 700603 were investigated by using the hollow agar
method. Zahterin ethanol and chloroform extracts were applied at 25mg / ml -
50mg / ml - 75mg / ml - 100mg / ml. 100% DMSO for negative control and
amoxicillin, clindamycin and penicillin for positive control were used.





According to the results; Zahter extract was found to be effective
against E. coli in its extract prepared with chloroform at concentrations of
25mg / ml-50mg / ml-75mg / ml and 100mg / ml (zone not seen, 18mm, 24mm, 27mm).
It was determined that Zahter extract inhibited the development of P. aeruginosa
in its extract prepared with chloroform at concentrations of 25mg / ml-50mg /
ml-75mg / ml and 100mg / ml (9mm, 11mm, 14mm, 27mm). Zahter extract was
observed to be effective against K. pneumoniae in its extract with chloroform
at concentrations of 25mg / ml-50mg / ml-75mg / ml and 100mg / ml (13mm, 17mm,
no zone, no zone). Zahter extract was found to be effective against K.
pneumoniae in its extract with ethanol at concentrations of 25mg / ml-50mg /
ml-75mg / ml and 100mg / ml (13mm, 17mm, no zone, no zone seen). But it did not
affect E. coli and P. aeruginosa. In addition, zahter extract with chloroform
was found to have a stronger antimicrobial effect than zahter extract made with
ethanol.

References

  • Al-Turki A.I., 2007. Antibacterial effect of thyme, peppermint, sage, black pepper and garlic hydrosols against Bacillus subtilis and Salmonella enteritidis. Journal of Food, Agriculture and Environment 5: 92–94.
  • Azriani, A.R., Siti, A.S., Zulkifli, A., Wan Nudri, W.D., Hamid, A.M., 2008. Prevalence and pattern of use of herbal medicines during pregnancy in tumpat district, kelantan. Malaysian Journal of Medical Sciences 15(3):40-48.
  • Bag, A., ve Chattopadhyay, R.R., 2015. Evaluation of Synergistic Antibacterial and Antioxidant Efficacy of Essential Oils of Spices and Herbs in Combination. PLos One. Jul 1;10(7).
  • Buwa, L., Van Staden, J., 2006. Güney Afrika'da zührevi hastalıklara karşı kullanılan geleneksel şifalı bitkilerin antibakteriyel ve antifungal aktivitesi J. Ethnopharmacol. 103, 139- 142.
  • Faydaoğlu, E., Sürücüoğlu, M.S., 2013. Tıbbi ve aromatik bitkilerin antimikrobiyal, antioksidan aktiviteleri ve kullanım olanakları. Erzincan University Journal of Science and Technology 6(2).
  • Gatta L, Perna F, Figura N, Ricci C, Holton J, D'Anna L, Miglioli M, Vaira D., 2003. Antimicrobial activity of esomeprazole versus omeprazole against Helicobacter pylori. J Antimicrob Chemother 51(2):439-42.
  • Gupta C., Garg A.P., Uniyal R.C., Kumari A., 2008. Comparative analysis of the antimicrobial activity of cinnamon oil and cinnamon extract on some food-borne microbes. African Journal of Microbiology Research. 247–251.
  • Güzeldag, G., Kadıoğlu, L., Açık, L., 2010. Antimicrobial Activity of Zahter Extract on Bacillus cereus and Staphylococcus aureus, Poster Sunumu, 6th Conference on Medicinal and Aromatic Plants of Southeast European Countries, ISSN: 0973-1296 6:22, 67-68.
  • Guzeldag, G., Kadioglu, L., Mercimek, A., Matyar, F., 2013. Preliminary examination of herbal extracts on the inhibition of Helicobacter pylori. African Journal of Traditional, Complementary and Alternative Medicines. 2;11(1): 93-6.
  • Husin, A., 2001. Adverse effects of herbs and drug-herbal interactions. Malaysian Journal of Pharmacy, 1(2): 39-44.
  • Liu, Q., Meng, X., Li, Y., Zhao, C.N., Tang, G.Y., Li, H.B., 2018. Antibacterial and Antifungal Activities of Spices. International Journal of Molecular Sciences 18(6), 1283.
  • Martini, S., D'Addario, C., Colacevich, A., Focardi, S., Borghını, F., Santucci, A., Figura, N., Rossi, C., 2009. Helicobacter pylori suşlarına ve böğürtlen yapraklarının (Rubus ulmifolius) ve izole edilmiş bileşiklerin antioksidan özelliklerine karşı antimikrobiyal aktivite. International Journal of Antimicrobial Agents 34 (1): 50-9.
  • Mutlu-Ingok, A., ve Karbancioglu-Guler, F., 2017. Cardamom, Cumin, and Dill Weed Essential Oils: Chemical Compositions, Antimicrobial Activities, and Mechanisms of Action against Campylobacter spp. Molecules. 22(7): 1191.
  • Nasar-Abbas, S.M., Halkman, A.K., 2004. Antimicrobial effect of water extract of sumac (Rhus coriaria L.) on the growth of some food borne bacteria including pathogens. International Journal of Food Microbiology 97(1):63-9.
  • Turkmen, F.U., Mercimek Takcı, H.A., Sekeroglu, N., 2016. Total Phenolic Content, Antioxidant and Antimicrobial Activities of Homemade and Industrial Samples of Breakfast Zahter. Herbal Mixture Akademik Gıda 14(3), 242-246.
  • Von Reis Altschul S. 1997. Exploring the herbarium Sci Am. 236(5):96-104.

Geleneksel Kahvaltılık Zahterin Antimikrobiyal Etkisi

Year 2020, Volume: 10 Issue: 1, 128 - 133, 15.01.2020
https://doi.org/10.17714/gumusfenbil.579489

Abstract

Günümüzde bazı hastalıkların tedavisinde
kullanılan preparatların etken maddeleri tamamen bitkisel kaynaklı
olabilmektedir. Bazı bitkilerin veya baharat karışımlarının antiviral, antibakteriyel
ve antifungal etkilerinin olduğu bilinmekte olup basit enfeksiyonların tedavi
edilmesinde veya koruyucu olarak bağışıklık sisteminin güçlendirilmesinde
takviye edici olarak kullanıldığı bilinmektedir. Çalışmada kullanılan zahter de
özellikle 
Güneydoğu Anadolu Bölgesi’nde kahvaltılık olarak tüketilen bir baharat
karışımıdır.

Bu çalışmada; zahterin Escherichia coli ATCC 25322, Pseudomonas
aeruginosa
DMS50071 SCOTTA, Klebsiella
pneumoniae
ATCC 700603’ya karşı oyuk agar yöntemi kullanılarak antimikrobiyal
etkileri incelendi. Zahterin etanol ve kloroform ekstreleri 25mg/ml - 50mg/ml -
75mg/ml - 100mg/ml olarak uygulandı. Negatif kontrol için %100 DMSO, pozitif
kontrol için de amoksisilin, klindamisin ve penisilin kullanıldı.





Sonuçlara göre; Zahter ekstraktının 25mg/ml-50mg/ml-75mg/ml ve 100mg/ml
konsantrasyonlarında (zon
görülmedi,18mm, 24mm, 27mm) kloroform ile hazırlanan ekstresinde E. coli’ye karşı etki ettiği belirlendi.
Zahter ekstraktının 25mg/ml- 50mg/ml-75mg/ml ve 100mg/ml konsantrasyonlarında
(9mm, 11mm, 14mm, 27mm) kloroform ile hazırlanan ekstresinde P. aeruginosa’ye gelişimini engellediği
tespit edildi. Zahter ekstraktının 25mg/ml- 50mg/ml-75mg/ml ve 100mg/ml
konsantrasyonlarında (13mm, 17mm, zon
yok, zon yok) kloroform ile hazırlanan ekstresinde K. pneumoniae karşı etki ettiği gözlendi. Zahter ekstraktının
25mg/ml- 50mg/ml- 75mg/ml ve 100mg/ml konsantrasyonlarında (13mm, 17mm, zon görülmedi, zon görülmedi) etanol
ile hazırlanan ekstresinde K. pneumoniae’ye
karşı etkili olduğu tespit edildi. Fakat E.
coli’
ye ve P. aeruginosa’ya karşı
etki etmedi. Ayrıca kloroform ile yapılan zahter ekstresinin, etanol ile
yapılan zahter ekstresine göre daha güçlü bir antimikrobiyal etkiye sahip olduğu
tespit edildi.

References

  • Al-Turki A.I., 2007. Antibacterial effect of thyme, peppermint, sage, black pepper and garlic hydrosols against Bacillus subtilis and Salmonella enteritidis. Journal of Food, Agriculture and Environment 5: 92–94.
  • Azriani, A.R., Siti, A.S., Zulkifli, A., Wan Nudri, W.D., Hamid, A.M., 2008. Prevalence and pattern of use of herbal medicines during pregnancy in tumpat district, kelantan. Malaysian Journal of Medical Sciences 15(3):40-48.
  • Bag, A., ve Chattopadhyay, R.R., 2015. Evaluation of Synergistic Antibacterial and Antioxidant Efficacy of Essential Oils of Spices and Herbs in Combination. PLos One. Jul 1;10(7).
  • Buwa, L., Van Staden, J., 2006. Güney Afrika'da zührevi hastalıklara karşı kullanılan geleneksel şifalı bitkilerin antibakteriyel ve antifungal aktivitesi J. Ethnopharmacol. 103, 139- 142.
  • Faydaoğlu, E., Sürücüoğlu, M.S., 2013. Tıbbi ve aromatik bitkilerin antimikrobiyal, antioksidan aktiviteleri ve kullanım olanakları. Erzincan University Journal of Science and Technology 6(2).
  • Gatta L, Perna F, Figura N, Ricci C, Holton J, D'Anna L, Miglioli M, Vaira D., 2003. Antimicrobial activity of esomeprazole versus omeprazole against Helicobacter pylori. J Antimicrob Chemother 51(2):439-42.
  • Gupta C., Garg A.P., Uniyal R.C., Kumari A., 2008. Comparative analysis of the antimicrobial activity of cinnamon oil and cinnamon extract on some food-borne microbes. African Journal of Microbiology Research. 247–251.
  • Güzeldag, G., Kadıoğlu, L., Açık, L., 2010. Antimicrobial Activity of Zahter Extract on Bacillus cereus and Staphylococcus aureus, Poster Sunumu, 6th Conference on Medicinal and Aromatic Plants of Southeast European Countries, ISSN: 0973-1296 6:22, 67-68.
  • Guzeldag, G., Kadioglu, L., Mercimek, A., Matyar, F., 2013. Preliminary examination of herbal extracts on the inhibition of Helicobacter pylori. African Journal of Traditional, Complementary and Alternative Medicines. 2;11(1): 93-6.
  • Husin, A., 2001. Adverse effects of herbs and drug-herbal interactions. Malaysian Journal of Pharmacy, 1(2): 39-44.
  • Liu, Q., Meng, X., Li, Y., Zhao, C.N., Tang, G.Y., Li, H.B., 2018. Antibacterial and Antifungal Activities of Spices. International Journal of Molecular Sciences 18(6), 1283.
  • Martini, S., D'Addario, C., Colacevich, A., Focardi, S., Borghını, F., Santucci, A., Figura, N., Rossi, C., 2009. Helicobacter pylori suşlarına ve böğürtlen yapraklarının (Rubus ulmifolius) ve izole edilmiş bileşiklerin antioksidan özelliklerine karşı antimikrobiyal aktivite. International Journal of Antimicrobial Agents 34 (1): 50-9.
  • Mutlu-Ingok, A., ve Karbancioglu-Guler, F., 2017. Cardamom, Cumin, and Dill Weed Essential Oils: Chemical Compositions, Antimicrobial Activities, and Mechanisms of Action against Campylobacter spp. Molecules. 22(7): 1191.
  • Nasar-Abbas, S.M., Halkman, A.K., 2004. Antimicrobial effect of water extract of sumac (Rhus coriaria L.) on the growth of some food borne bacteria including pathogens. International Journal of Food Microbiology 97(1):63-9.
  • Turkmen, F.U., Mercimek Takcı, H.A., Sekeroglu, N., 2016. Total Phenolic Content, Antioxidant and Antimicrobial Activities of Homemade and Industrial Samples of Breakfast Zahter. Herbal Mixture Akademik Gıda 14(3), 242-246.
  • Von Reis Altschul S. 1997. Exploring the herbarium Sci Am. 236(5):96-104.
There are 16 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Articles
Authors

Semih Dalkılıç 0000-0002-6892-247X

Lütfiye Kadıoğlu Dalkılıç 0000-0002-6791-3811

İsmail Korkmaz 0000-0003-4631-7786

Publication Date January 15, 2020
Submission Date June 18, 2019
Acceptance Date October 23, 2019
Published in Issue Year 2020 Volume: 10 Issue: 1

Cite

APA Dalkılıç, S., Kadıoğlu Dalkılıç, L., & Korkmaz, İ. (2020). Geleneksel Kahvaltılık Zahterin Antimikrobiyal Etkisi. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 10(1), 128-133. https://doi.org/10.17714/gumusfenbil.579489