HEMOGLOBİN VE NİTRİT BAĞIMLI TİROZİN NİTRASYONU

Year 2017, Volume: 6 Issue: 1, 46 - 57, 31.03.2017

Hasan Karageçili , Kamer Kılınç

Abstract

Nitrik oksit (NO) oksihemoglobin ve
oksimiyoglobin ile tepkimeye girmesi nitrat ve metHb veya metMb'nin meydana
gelmesine neden olmaktadır. Nitratin tersine, nitrit NO oksidasyonunun zararsız
bir son ürünü değildir. Hemoproteinlerden peroksidazların H₂O₂
varlığında nitrit oksidasyonuna neden oldukları gösterilmiştir. Hemoproteinlerin
katalizlediği nitrit oksidasyonunda meydana gelen reaktif nitrojen oksitler,
nitritin bakterisid ve sitotoksik etkilerinin sorumlusudurlar. Bu çalışmada
Griess metodu ile hemoglobin tarafından H₂O₂ varlığında
nitritin nitrata oksidasyonu araştırıldı.

Hem H₂O₂ hem de
hemoglobin yokluğunda nitrit oksidasyonu gözlenmedi, bu da göstermektedir ki
nitrit oksidasyonuna neden olan reaktif tür H₂O₂ ile
hemoglobinin reaksiyonundan meydana gelen oksoferril kompleksidir (bileşik I).
Bu çalışmada, peroksidaz enzimlerine benzer şekilde, hemoglobinin tirozin
nitrasyonuna neden olduğu bulundu. Hemoglobinin katalizlediği tirozin
nitrasyonu pH bağımlı olup, nitrasyonun optimum pH'si 6,0 dır. Hemoglobin ve H₂O₂
tirozin nitrasyonu için gerekli bileşenlerdir. Hemoglobin yokluğunda, çalışılan
en düşük pH'de bile tirozin nitrasyonu gözlenmedi. Bununla beraber H₂O₂
varlığında hemoglobinin katalizlediği nitrit oksidasyonu esnasında,
nitratlayıcı türler meydana getirilmektedir.

Sonuç olarak hemoglobin, H₂O₂
ve nitrit arasındaki reaksiyon serileri tarafından nitrojen dioksit radikali
(NO₂^-) ve/veya
peroksinitrit benzeri reaktif türler meydana getirilmektedir ve bu türler
tirozin nitrasyonundan sorumludurlar.

Keywords

Nitratlar, Hemoglobinler, Nitritler, Nitrik Oksit

References

• Connolly, D., Paull B. (2001). “Rapid determination of nitrate and nitrite in drinking water samples using ion-interaction liquid chromatography”. Analytica Chimica Acta ; 441:53–62.
• Mikuška, P., Večeřa Z. (2003). “Simultaneous determination of nitrite and nitrate in water by chemiluminescent flow-injection analysis”. Analytica Chimica Acta ;495:225–232.
• Biswas, S., Chowdhury, B., Ray, B. C. (2004). “A novel spectrofluorimetric method for the ultra trace analysis of nitrite and nitrate in aqueous medium and its application to air, water, soil and forensic samples”. Talanta :64:308–312.
• Vatassery, G. T., SantaCruz, K. S., DeMaster, E. G., Quach, H. T, Smith, W. E. (2004). “Oxidative stress and inhibition of oxidative phosphorylation induced by peroxynitrite and nitrite in rat brain subcellular fractions”. Neurochemistry International;45:963-970.
• Kleinbongard, P., Dejam, A., Lauer, T., Rassaf, T., Schindler A., Picker, O., Scheeren, T., Gödecke, A., Schrader, J., Schulz, R., Heusch, G., Schaub, G. A., Bryan, N. S., Feelisch, M., Kelm, M. (2003). “Plasma nitrite reflects constitutive nitric oxide synthase activity in mammals”. Free Radical Biology & Medicine ;35(7):790–796.
• Okafor, P. N., Ogbonna, U. I. (2003). “Nitrate and nitrite contamination of water sources and fruit juices marketed in South-Eastern Nigeria”. Journal of Food Composition and Analysis ;16:213 –218.
• Pannala, A. S., Mani, A., Spencer, J. P. E., Skinner, V., Bruckdorfer, K. R., Moore, K. P., Rice-Evans, C. A. ( 2003). “The effect of dietary nitrate on salivary, plasma, and urinary nitrate metabolism in humans”. Free Radical Biology & Medicine ;34(5):576–584.
• Pinto P. C. A. G., Lima, J. L. F. C., Saraiva, M. L. M. F. S. (2003). “Sequential injection analysis of nitrites and nitrates in human serum using nitrate reductase”. Clinica Chimica Acta ;337: 69–76.
• Van der Vliet, A., Eiserich, J. P., Shigenaga, M. K, Cross, C. E. (1999). Reactive Nitrogen Species and Tyrosine Nitrationin the Respiratory Tract”. Am J Respir Crit Care Med ;160:1–9.
• Holm, P. (2000). “Nitric Oxide and Peroxynitrite Production by NO-donors and Inflammatory Cells”. Printed dissertation Acta Universitatis Tamperensis ;771:951-44-4931-2.
• Jane, D. R., Llobera, M., Tejero Lo' pez, M. D. (2002). “Anticoagulants and Other Preanalytical Factors Interfere in Plasma Nitrate/Nitrite Quantification by the Griess Method”. Nitric Oxide: Biology and Chemistry ;6(2):178-185.
• Ridnour, L. A., Sim, J. E., Hayward, M. A., Wink, D. A., Martin, S. M., Buettner, G. R., Spitz D. R. (2000). “A Spectrophotometric Method for the Direct Detection and Quantitation of Nitric Oxide, Nitrite, and Nitrate in Cell Culture Media”. Analytical Biochemistry ;281:223-229.
• Herold, S. (2004). “Nitrotyrosine, Dityrosine, and Nitrotryptophan Formation From Metmyoglobin, Hydrogen Peroxide, and Nitrite”. Free Radical Biology & Medicine ;36(5):565 – 579.
• Burakham, R., Oshima, M., Grudpan, K., Motomizu, S. (2004). “Simple flow-injection system for the simultaneous determination of nitrite and nitrate in water samples”. Talanta.
• Lundberg, J. O., Giovani M. (2004). “Inorganic nitrate is a possible source for systemic generation of nitric oxide”. Free Radical Biology & Medicine ;37(3): 395-400.
• Byun, J., Henderson, J. P., Mueller, D. M., Heinecke, J. W. (1999). “8-Nitro-2'-deoxyguanosine, a specific Marker of oxidation by reactive nitrogen species, Is generated by the myeloperoxidase-hydrogen peroxide-nitrite system of activated human phagocytes”. Biochemistry ;38:2590-2600.
• Takahama, U., Hirota, S., Nishioka, T., Oniki, T. (2003). “Human salivary peroxidase-catalyzed oxidation of nitrite and nitration of salivary components 4-hydroxyphenylacetic acid and proteins”. Archives of Oral Biology ;48:679-690.
• Feng, X., Li, C., Jia, X., Guo, Y., Lei, N., Hackman, M. R., Chen, L., Zhou, G. (2016). “Influence of sodium nitrite on protein oxidation and nitrosation of sausages subjected to processing and storage”. Meat Science ;116:260–267.
• Rocha, S. B., Gago, B., Barbosa, M. R., Lundberg, O. J., Mann, E. G., Radi, R., Laranjinha, J. (2013). “Pepsin is nitrated in the rat stomach, acquiring antiulcerogenic activity: A novel interaction between dietary nitrate and gut proteins”. Free Radical Biology and Medicine ;58:26–34.
• 20 . Van der Vliet, A., Eiserich, J. P., Halliwel, B., Cross, C. E. (1997). “Formation of Reactive Nitrogen Species during Peroxidase-catalyzed Oxidation of Nitrite”. Journal of Biological Chemistry ;272(12):7617–7625.
• Wu, W., Chen, Y., Hazen, S. L. (1999). “Eosinophil peroxidase nitrates protein tyrosine residues”. The Journal of Biological Chemistry ;274(36):25933-25944.
• Sampson, J. B, Ye, Y., Rosen, H., Beckman, J. S. (1998). “Miyeloperosidase and horseradish peroxidase catalyzed tyrosine in proteins from nitrite and hyrgogen peroxidase”. Archieves of Biochemistry and Biophysics ;356(2):207-213.
• Davis, I. C., Zhu, S., Sampson, J. B., Crow, J. P., Matalon S. (2002). Inhibiton of Human Surfectant Protein Afunction by oxidation intermediates of nitrite”. Free Radical Biology & Medicine ;33(12):1703-1713.
• Schopfer, F. J., Baker, P. R. S., Freeman, B. A. (2013). “NO-dependent protein nitration: cellsignaling event or an oxidative inflamatory response. Trends in Biochemical Sciences ;28:12.
• Turko I. V., Murad, F. “Protein Nitration in Cardiovascular Diseases”. (2002). Pharmacol Rev ;54(4):619–634.
• Kılınç, K., Kılınç, A., Wolf, R. E., Grisham, M. B. (2001). “Myoglobin-Catalyzed Tyrosine Nitration: No Need for Peroxynitrite”. Biochemical and Biophysical Research Communications ;285:273–276.
• Grzelak, A., Balcerczyk, A., Mateja, A., Bartosz, G. (2001). Hemoglobin can nitrate itself and other proteins”. Biochimica et Biophysica Acta ;1528:97-100.
• Daiber, A., Mehl, M., Ulrich, V. (1998). “New aspects in the reaction mechanism of phenol with peroxynitrite: The role of phenoxy radicals”. Nitric Oxide: Biology and Chemistry; 2(4) 259-269.
• Pecci, L., Montefoschi, G., Antonucci, A., Costa M., Fontana, M., Cavalliano, D. ( 2001). “Formation of nitrotyrosine by methylene blue photosensitized oxidation of tyrosine in the presence of nitrite”. Biochemical and Biophysical Research Communications;289:305-309.
• Bian, K., Gao, Z., Weisbrodt, N., Murad F. (2003). “The nature of heme/iron-induced protein tyrosine nitration”. PNAS ;100(10):5712–5717.