Effect of White Noise and Light Exposure on Some Enzymes in Rat Testicular Tissue

Year 2023, Volume: 12 Issue: 3, 889 - 894, 26.09.2023

Esra Şentürk , Hilal Üstündağ , İmdat Aygül , Murat Şentürk

https://doi.org/10.37989/gumussagbil.1323937

Abstract

By definition, white noise (WN) is a type of sound that consists of a combination of various frequencies. Noise is an important source of environmental stress for humans and other living things and has a strong stimulating effect on light, circadian rhythm, hormonal, and neuro-behavioral levels. When faced with stressful situations, organisms produce a substantial quantity of free radicals, leading to alterations in the oxidative system. The objective of this research is to examine how environmental stressors like WN and light impact the activities of enzymes involved in testicular carbonic anhydrase (CA), catalase (CAT), and glutathione peroxidase (GPx). A total of 18 male Sprague-Dawley rats were divided into three groups. The first was the control group (not exposed to stress, kept at 12/12 light-dark cycle and 24±2°C normal ambient temperature). The second group, known as the WN 60' group, experienced 60 minutes of white noise exposure per day for 14 consecutive days at 90 dB. The third group, referred to as the WN 60' + non daylight group, underwent 60 minutes of white noise exposure at 90 dB combined with 24 hours of continuous light exposure every other day. At the conclusion of the study, the testicular tissues were homogenized and centrifuged, followed by the spectrophotometric measurement of enzyme activities.
As a result, when we compared the control group with both WN and WN + light exposure; A decrease was observed for CA enzyme in both applications, and an increase was observed in CAT and GPx enzymes in both applications.
As a result, when we compared the control group with both BG and BG + light exposure; A decrease was observed for CA enzyme in both applications, and an increase was observed in CAT and GPx enzymes in both applications.

Keywords

White Noise, Enzyme, Testicle

Beyaz Gürültü ve Işık Maruziyetinin Sıçan Testis Dokusundaki Bazı Enzimler Üzerine Etkisi

Abstract

Tanım olarak beyaz gürültü (BG), çeşitli frekansların birleşiminden oluşan bir ses türüdür. Gürültü insanlar ve diğer canlılar için önemli bir çevresel stres kaynağıdır ve ışık, sirkadiyen ritim, hormonal ve nöro-davranış düzeylerinde güçlü bir uyarıcı etkisi vardır. Stres maruziyetine yanıt olarak canlılar büyük ölçüde serbest radikaller üretir, bu da oksidatif sistemde değişikliklere neden olur. Bu çalışmanın amacı, beyaz gürültü ve ışık gibi çevresel stres faktörlerinin testis karbonik anhidraz (CA), katalaz (CAT) ve glutatyon peroksidaz (GPx) enzimlerinin aktiviteleri üzerindeki etkisini belirlemektir. 18 Sprag Dawley erkek rat 3 gruba ayrıldı. Birinci grup, Kontrol grubu (strese maruz kalmadan, 12/12 aydınlık-karanlık döngüsü, 24±2°C normal ortam sıcaklığı), ikinci grup BG 60' grubu (14 gün boyunca günde 60 dakika 90 dB'de BG)) ve üçüncü grup BG 60' + gün aşırı ışık grubu (90 dB'de 60 dakika/gün BG maruziyeti ve gün aşırı 24 saat sürekli ışık maruziyeti). Çalışma sonunda testis dokuları homojenize edilerek santrifüj edildi. Enzim aktiviteleri spektrofotometrik olarak ölçüldü.
Sonuç olarak hem BG hem de BG + ışık maruziyeti uygulanan gruplarda kontrol ile kıyasladığımız zaman; CA enzimi için her iki uygulamada da düşüş, CAT ve GPx enzimlerinde ise her iki uygulamada da atrış olduğu gözlenmiştir.
Sonuç olarak, hem BG hem de BG + ışık maruziyeti uygulanan gruplarda kontrol ile kıyasladığımız zaman; CA enzimi için her iki uygulamada da düşüş, CAT ve GPx enzimlerinde ise her iki uygulamada da atrış olduğu gözlenmiştir.

Keywords

Beyaz Gürültü, Enzim, Testis

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