Septoria yaprak leke hastalığı etmeni Zymoseptoria tritici (Desm. Quaedvlieg & Crous)’ye ait izolatların farklı sıcaklıklardaki fizyolojik ve biyokimyasal özelliklerin belirlenmesi

Year 2021, Volume: 25 Issue: 4, 469 - 479, 25.12.2021

Nevzat Kılınç , Murat Dikilitaş , Mukaddes Kayım , Gülsüm Ünal

https://doi.org/10.29050/harranziraat.897692

Cited By: 1

Abstract

Bu çalışma ileseptoria yaprak leke hastalığı etmeni Zymoseptoria tritici(Desm. Quaedvlieg & Crous)’ye ait 4 izolatın farklı sıcaklık koşullarında (4-, 15-, 25-, 30-, 35°C) fizyolojik (birim alandaki spor yoğunluğu, miselyal gelişim ve birim hacimdeki spor sayısı) ve biyokimyasal (protein, prolin, proteaz, melanin ve malonadialdehit) değişimleri incelenmiştir. Sıcaklık değerleri optimum (25°C) değerden uzaklaştıkça patojenin fizyolojik ve biyokimyasal değerlerinde değişimler tespit edilmiştir. Birim alandaki spor yoğunluğu, ortalama büyüme çapı ve birim hacimdeki spor sayısında azalma olmuştur(P≤0.05). Yüksek sıcaklıkta (30-, 35°C) patojene ait protein miktarı azalmış, prolin, proteaz, melanin ve malonadialdehit (MDA) değerlerinde ise artış görülmüştür. Buna bağlı olarak fungus izolatları arasında gelişme oluştuğu saptanmıştır. Patojenin CU100StA4 nolu izolatı birim alandaki spor yoğunluğu, ortalama büyüme çapı, birim hacimdeki spor sayısı ve ürettiği proteaz miktarı itibari ile diğer izolatlardan pozitif olarak ayrışmıştır. Patojenin CU107StH110 nolu izolatının prolin, melanin ve malonadialdehit değerleri, diğer izolatlardan daha yüksek çıkmıştır. Yüksek sıcaklıkta (30-, 35°C) proteaz enziminin üretilmesi ile uygun nem koşullarında fungusun virülensliğinin devam edebileceği gözlemlenmiştir. Fungusun proteaz enzim üretimi ile misel uzunluğu, protein ve prolin miktarları arasında pozitif bir ilişki olduğu belirlenmiştir.

Keywords

Zymoseptoria tritici, sıcaklık, proteaz, Melanin, Malonadialdehit (MDA)

Determination of physiological and biochemical characteristics of isolates of septoria leaf blotch disease agent [Zymoseptoria tritici(Desm. Quaedvlieg &Crous)] at different temperatures

Abstract

In this study, 4 isolates belonging to septoria leaf spot disease [Zymoseptoria tritici (Desm. Quaedvlieg & Crous)] fungus and physiological (spore density per unit area) under different temperature conditions (4-, 15-, 25-, 30-, 35 ° C) micellar growth and the number of spores per unit volume) and biochemical (protein, proline, protease, melanin and malonadialdehyde) changes were investigated. Spore density per unit area, average growth diameter and number of spores per unit volume decreased. Proline, protease, melanin and malonadialdehyde (MDA) values increased, while protein content of the pathogen decreased at high temperature (30-, 35°C).Accordingly, it has been determined that there are differences in growth among the fungus isolates. The isolate numbered CU100StA4 of the pathogen was found to be higher than the other isolates in terms of spore density per unit area, average growth, diameter, number of spores per unit volume and the amount of protease produced. Proline, melanin and malonadialdehyde values of the isolate of the pathogen no. CU107StH110 were higher than the other isolates. It was observed that the presence of protease enzyme at high temperature (30-, 35°C) and the virulence of the fungus could continue under suitable humidity conditions. It has been evaluated that there is a positive relationship between fungus protease enzyme production and micelle length, protein and proline values.

Keywords

Zymoseptoria tritici, temperature, protease, Melanin, malondialdehyde (MDA)

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