IGF-1/CaC8I, LEP/NmuCI and PRLR/Hin1II Polymorphisms in Saanen Goats

Year 2022, Volume: 63 Issue: 1, 15 - 20, 01.07.2022

Mustafa Can Yılmaz , Prof.dr.güldehen Bilgen

https://doi.org/10.29185/hayuretim.1018577

Abstract

Objective: This study was aimed to identify IGF-1, LEP and PRLR polymorphisms by PCR-RFLP method in Saanen goats.
Material and Methods: In the study, nasal samples were collected from 72 Saanen goats via swabs and DNA was extracted. Polymorphisms of IGF-1, LEP and PRLR were determined by PCR amplification followed by RFLP method using restriction enzymes Cac8I, NmuCI and Hin1II, respectively.
Results: LEP/NmuCI produced three genotypes as TT, TC and CC with frequencies 90.3, 6.9 and 2.8%, respectively and two alleles (T=93.8%, C=6.2%). The frequencies of genotype for PRLR gene were CC (90.3%), CT (5.5%) and TT (4.2%) and allele frequencies were C (93.1%) and T (6.9%). The IGF-1/Cac8I locus was monomorphic in Saanen goat population.
Conclusion: The present results may be useful for future studies conducted on goat genome with respect to IGF-1, LEP and PRLR genes.

Keywords

DNA markers, PCR-RFLP, single nucleotide polymorphism, nasal swab

Supporting Institution

Ege Üniversitesi ÖYP Birimi

Thanks

This paper is a part of the PhD thesis of Mustafa Can YILMAZ who was supported by Faculty Member Training Program (ÖYP).

Saanen Keçilerinde IGF-1/Cac8I, LEP/NmuCI ve PRLR/Hin1II Polimorfizmleri

Abstract

Amaç: Bu çalışmada Saanen keçilerinde IGF-1, LEP ve PRLR polimorfizmlerinin PCR-RFLP yöntemi ile saptanması amaçlanmıştır.
Materyal ve Yöntem: Çalışmada, 72 Saanen keçisinden sürüntü yoluyla burun içi epitel hücre örnekleri toplanmış ve DNA izolasyonu yapılmıştır. IGF-1, LEP ve PRLR polimorfizmleri, sırasıyla Cac8I, NmuCI ve Hin1II restriksiyon enzimleri kullanılarak PCR amplifikasyonunu takiben RFLP yöntemi ile belirlenmiştir.
Bulgular: LEP/NmuCI lokusunda genotip frekansları TT (%90.3), TC (%6.9) ve CC (%2.8) ve allel frekansları T (%93.8), C (%6.2) şeklinde tespit edilmiştir. PRLR/Hin1II için genotip frekansları CC (%90.3), CT (%5.5), CC (%4.2) ve allel frekansları C (%93.1) ve T (%6.9) olarak hesaplanmıştır. IGF-1/Cac8I lokusunun popülasyonumuzda monomorfik olduğu tespit edilmiştir.
Sonuç: Bu sonuçların IGF-1, LEP ve PRLR genleri ile ilgili olarak keçi genomu üzerinde yapılacak gelecekteki çalışmalar için faydalı olabileceği düşünülmektedir.

Keywords

DNA belirteçleri, PCR-RFLP, tek nükleotid polimorfizmi, nazal sürüntü

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