COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS

Year 2023, Volume: 51 Issue: 4, 327 - 340, 07.11.2023

Ece Battaloğlu , Zehra Gül Morçimen , Aylin Şendemir

https://doi.org/10.15671/hjbc.1245678

Abstract

Gliosis is a condition that plays a negative role in various neurological pathologies. In this study, it was aimed to obtain and compare the glio-protective effects of electrospun fibrous scaffolds coated by different biopolymers. First, in vitro gliosis model was obtained by inducing U-87 MG cells with LPS and IFN-γ. The optimized model was applied to U-87 MG cells grown on polycaprolactone (PCL), hyaluronic acid (HA) coated, gelatin coated, and collagen coated PCL scaffolds. Immunofluorescent (IF) staining was performed with glial fibrillary acidic protein (GFAP) antibody to determine the level of gliosis. It was found that 5µg/mL LPS concentration induced gliosis and hyaluronic acid-coated PCL scaffolds have shown a protective effect on gliosis.

Keywords

Gliosis, neural tissue engineering, electrospinning, glio-protective

Thanks

We would like to thank BUGAMED BIOTECHNOLOGY for providing hyaluronic acid as a gift. Also, we would like to thank Prof. Dr. Ayse NALBANTSOY for her support in cell culture experiments.

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