Nanoscale Liposome Synthesis for Drug Delivery Applications via Ultrafast Acoustofluidic Micromixing

Year 2023, Volume: 10 Issue: 3, 237 - 241, 30.09.2023

Ali Pourabdollah Vardin , Gurkan Yesıloz

https://doi.org/10.17350/HJSE19030000312

Cited By: 1

Abstract

Nowadays, lipid nanoparticles have gained profound interest in chemical and biomedical engineering. The rapid development of therapeutic nanosystems has led to a need to design suitable approaches to synthesize bio-carriers for efficient drug delivery. Microfluidic methods provide an excellent opportunity to acquire desirable nanoparticle properties, including stability, size, shape, and size distribution, which are often challenging to obtain using conventional bulk synthesis methods. Rapid mixing is a crucial factor in the nanoprecipitation process as it influences the size and size distribution of the nanoparticles. Within this regard, in this work, we report an ultrafast acoustofluidic micromixer to synthesize liposome nanoparticles, which have been widely investigated in the literature as drug carriers due to their biocompatibility and biodegradability. This research has also investigated the influence of glycerol addition to the solvent to control the size of the liposomes. Our findings indicate that utilizing the acoustofluidic platform resulted in the production of nanoscale liposomes with small mean sizes compared to the hydrodynamic flow-focusing (HFF) method. Furthermore, the inclusion of glycerol led to a significant reduction in liposome size. These results emphasize the potential of the proposed approach for the efficient and precise synthesis of liposome nanoparticles with improved characteristics, which can be utilized in various biomedical and drug delivery applications.

Keywords

Acoustofluidics, Liposome synthesis, Acoustic micromixers, Microfluidics

Supporting Institution

TÜBİTAK

Project Number

118C263

Thanks

Dr. Gurkan Yesiloz gratefully acknowledges the support from the Scientific and Technological Research Council of Türkiye (TUBITAK) (2232-Grant No: 118C263) and (1001-Grant No: 221M575). However, the entire responsibility of the publication/article belongs to the owner of the publication/article. The financial support received from TUBITAK does not mean that the content of the publication is approved in a scientific sense by TUBITAK.

References

• 1. Kohane DS. Microparticles and nanoparticles for drug delivery. Biotechnology and Bioengineering. 96(2) (2007):203-9.
• 2. Torchilin, Vladimir P. Nanoparticulates as drug carriers. Imperial college press, (2006).
• 3. Mitragotri, Samir and Patrick Stayton. Organic nanoparticles for drug delivery and imaging. Mrs Bulletin 39.3 (2014): 219-223.
• 4. Immordino, Maria Laura, Franco Dosio, and Luigi Cattel. Stealth liposomes: review of the basic science, rationale, and clinical applications, existing and potential. International journal of nanomedicine. 1.3 (2006): 297.
• 5. Çağdaş, Melis, Ali Demir Sezer and Seyda Bucak. Liposomes as potential drug carrier systems for drug delivery. Application of nanotechnology in drug delivery 1 (2014): 1-50.
• 6. Brandl, M. Liposomes as drug carriers: a technological approach. Biotechnol Annu Rev (2001): 59-85.
• 7. Mallick, Sudipta, and Joon Sig Choi. Liposomes: versatile and biocompatible nanovesicles for efficient biomolecules delivery. Journal of nanoscience and nanotechnology 14.1 (2014): 755-765.
• 8. Laouini, Abdallah, et al. Preparation, characterization and applications of liposomes: state of the art. Journal of colloid Science and Biotechnology 1.2 (2012): 147-168.
• 9. Akbarzadeh, Abolfazl, et al. Liposome: classification, preparation, and applications. Nanoscale research letters 8.1 (2013): 1-9.
• 10. Tian, Fei, et al. Microfluidic technologies for nanoparticle formation. Lab on a Chip (2022) 22, 512-529.
• 11. Valencia, Pedro M., et al. Microfluidic technologies for accelerating the clinical translation of nanoparticles. Nature nanotechnology 7.10 (2012): 623-629.
• 12. Ma, Junping, et al. Controllable synthesis of functional nanoparticles by microfluidic platforms for biomedical applications–a review. Lab on a Chip 17.2 (2017): 209-226.
• 13. Zhang, Guo, and Jiaming Sun. Lipid in chips: a brief review of liposomes formation by microfluidics. International Journal of Nanomedicine 16 (2021): 7391.
• 14. Tan, Yung-Chieh, et al. Controlled microfluidic encapsulation of cells, proteins, and microbeads in lipid vesicles. Journal of the American Chemical Society 128.17 (2006): 5656-5658.
• 15. Yu, Bo, Robert J. Lee, and L. James Lee. Microfluidic methods for production of liposomes. Methods in enzymology 465 (2009): 129- 141.
• 16. Michelon, Mariano, et al. High-throughput continuous production of liposomes using hydrodynamic flow-focusing microfluidic devices. Colloids and Surfaces B: Biointerfaces 156 (2017): 349-357.
• 17. Hood, Renee R. and Don L. DeVoe. High‐Throughput Continuous Flow Production of Nanoscale Liposomes by Microfluidic Vertical Flow Focusing. Small 11.43 (2015): 5790-5799.
• 18. Han, Jung Yeon, Joseph N. La Fiandra and Don L. DeVoe. Microfluidic vortex focusing for high throughput synthesis of sizetunable liposomes. Nature Communications 13.1 (2022): 6997.
• 19. Shan, Han, et al. One‐Step Formation of Targeted Liposomes in a Versatile Microfluidic Mixing Device. Small 19.7 (2023): 2205498.
• 20. Rasouli, M. Reza, and Maryam Tabrizian. An ultra-rapid acoustic micromixer for synthesis of organic nanoparticles. Lab on a Chip 19.19 (2019): 3316-3325.
• 21. Zhao, Shuaiguo, et al. Fabrication of tunable, high-molecularweight polymeric nanoparticles via ultrafast acoustofluidic micromixing. Lab on a Chip 21.12 (2021): 2453-2463.
• 22. Modarres, Paresa, and Maryam Tabrizian. Electrohydrodynamicdriven micromixing for the synthesis of highly monodisperse nanoscale liposomes. ACS Applied Nano Materials 3.5 (2020): 4000-4013.
• 23. Yesiloz, Gurkan, Muhammed Said Boybay and Carolyn L. Ren. Label-free high-throughput detection and content sensing of individual droplets in microfluidic systems. Lab on a Chip 15.20 (2015): 4008-4019.
• 24. Yesiloz, Gurkan, Muhammed S. Boybay and Carolyn L. Ren. Effective thermo-capillary mixing in droplet microfluidics integrated with a microwave heater. Analytical chemistry 89.3 (2017): 1978-1984.
• 25. Danaei, M., et al. Impact of particle size and polydispersity index on the clinical applications of lipidic nanocarrier systems. Pharmaceutics 10.2 (2018): 57.