Oral Süspansiyondaki Sülfametoksazol ve Trimetoprimin Klasik Küçük Kare Kalibrasyon Metodu ile Spektrofotometrik Tayinleri ve Stabilite Çalışmaları

Year 2009, Issue: 2, 95 - 104, 01.06.2009

Şahver Ege Hişmioğulları , Ender Yarsan

Abstract

Oral süspansiyondaki sülfametoksazol ve trimetoprimin kantitatif tayini ve stabilite testi, spektrofotometrik klasik küçük kare kalibrasyon metodu ile çalışıldı. Sözkonusu veteriner ilaçlarının stabilite testleri, şu koşullarda gerçekleştirildi : Bir yıl süresince, oda ısısı, buzdolabı ve etüvde 37 0C bekletilen ilaçların, her üç ayda bir, analizleri yapıldı. Spektrofotometrik analiz için sülfametoksazol ve trimetoprimin, sırasıyla, 2.0-11.0 μg/mL ve 3.0-18.0 μg/mL konsantrasyon aralığındaki standart serileri hazırlandı. Standart serilerin absorbansları, üç ayrı dalga boyunda ölçüldü 237, 257 ve 288 nm . Bir klasik küçük kare kalibrasyonu, üç ayrı dalga boyunda absorbtiviteler ölçülerek elde edildi. Spektral metodun validasyonu, sentetik ikili karışımlar analiz edilerek yapıldı. Ardından da metot, sülfametoksazol ve trimetoprim içeren ticari veteriner formülasyonun eş zamanlı simultane analizi ve stabilite testine başarıyla uygulandı.

Keywords

Spektral klasik küçük kare, Simultane tayin, Stabilite testi, Sülfametoksazol, Trimetoprim, Kemometri

Spectrophotometric Determination and Stability Studies of Sulfamethoxazole and Trimethoprim in Oral Suspension by Classical Least Square Calibration Method

Abstract

Quantitative determination and stability test of sulfamethoxazole and trimethoprim in oral suspension were carried out by spectrophometric classical least square calibration method. Stability test of the related veterinary drugs was performed by using the following conditions: Room temperature, refrigerator and etuve 37 0C for each 3 month during 12 months. For the spectrophotometric analysis, the standard series of sulfamethoxazole and trimethoprim was prepared in the concentration range of 2.0-11.0 μg/mL and 3.0-18.0 μg/mL respectively. The absorbances of standard series were measured at three-wavelength set 237, 257 and 288 nm . A classical least square calibration was obtained by using the absorptivities at three-wavelength set. The validity of the spectral method was done by analyzing the synthetic binary mixtures. Af terwards the method was successfully applied to simultaneous analysis and stability test of the commercial veterinary formulation containing sulfamethoxazole and trimethoprim.

Keywords

Spectral classical least square, Simultaneous determination, Stability test, Sulfamethoxazole, Trimethoprim, Chemometry

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