UV kombine RP-HPLC ile İzotonik Serum Fizyolojikte İnsülin Tayini

Year 2021, Volume: 41 Issue: 2, 74 - 81, 01.06.2021

Merve Nenni

https://doi.org/10.52794/hujpharm.901476

Abstract

Tip I diabetes mellitus tedavisinde insülin yaygın olarak intravenöz olarak kullanılmaktadır. Bu çalışmanın amacı, % 0.9’luk NaCl solüsyon örneklerinde insülin tayini için yenilikçi, hızlı ve basit bir analiz tekniği geliştirmektir. İnsülin, UV detektörlü HPLC ile analiz edilmiştir. Yöntem, FDA yönergelerine göre onaylanmış ve tatmin edici sonuçlar gözlenmiştir. Örnekler, ACE 5 um C18 100 Å LC kolonu (250 x 4.6 mm) kullanılarak HPLC sistemine manuel olarak enjekte edilmiştir. Mobil faz, bir asetonitril: fosfat tamponu (pH 3.0, 10 mM) (50:50 v / v) karışımıdır. Enjeksiyon hacmi 20 µL’dir. UV saptaması 240 nm’de gerçekleştirilmiştir. Yöntemin, özgün, doğrusal, kesin, doğru ve duyarlı bir yöntem olduğu validasyon çalışmalarıyla ortaya konulmuştur.

Keywords

Reguler insulin, HPLC, izotonik serum fizyolojik, validasyon, UV

Determination of Insulin in saline by RP-HPLC combined with UV

Abstract

In the treatment of type I diabetes mellitus, insulin is widely used intravenously. This study aims to develop an innovative, fast and simple analysis technique for insulin determination in 0.9% NaCl solution samples. Insulin was analyzed by HPLC with a UV detector. The method has been validated according to FDA guidelines and satisfactory results have been observed. Samples were manually injected into the HPLC system using ACE 5 µm C18 100 Å LC column (250 x 4.6 mm). The mobile phase was a mixture of acetonitrile: phosphate buffer (pH 3.0, 10 mM) (50:50 v/v). The injection volume is 20 µL. UV detection was carried out at 240 nm. Based on validation studies, the method was found as specific, linear, precise, accurate, and sensitive.

Keywords

Regular insulin, HPLC, saline solution, validation, UV

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