Sıçanlarda Tıkanma Sarılığında Melatonin ve N-Asetilsisteinin Etkileri

Year 2022, Volume: 42 Issue: 1, 22 - 30, 01.03.2022

Mehmet Fatih Ekici , Alper Akcan , Hızır Yakup Akyıldız , Kemal Deniz Recep Saraymen Yücel Arıtaş

https://doi.org/10.52794/hujpharm.1034938

Abstract

Amaç: Tıkanma sarılıklı sıçanlarda, koletastazda N–asetilsistein ve melatoninin etkilerini ve karaciğer ve böbrek hasarında koruyucu rollerini araştırmak ve karşılaştırmak amaçlandı.
Materyal ve Metod: 48 sıçan çalışma içinkullanıldı. Sıçanlar sham, ana kontrol ve çalışma olarak 3 ana gruba ayrıldı. Daha sonra ana kontrol grubu erken sakrifikasyon grubu ve kontrol grubuna ayrıldı. Çalışma grupları kendi içinde melatonin, N–asetilsistein ve melatonin & N–asetilsistein olmak üzere üç ayrı alt gruba ayrıldı. Çalışma ve kontrol gruplarında laparotomi yapıldı, koledok dönüldü, bağlandı ve kesildi. İşlemden 5 gün sonra erken sakrifikasyon grubunda; kan örnekleri, karaciğer ve böbrek dokuları alındı. Çalışma gruplarında beşinci günden başlayarak 10. güne kadar subkutan olarak melatonin, N–asetilsistein ve melatonin & N–asetilsistein uygulandı, sham ve kontrol gruplarına serum fizyolojik verildi. 10. Günde tüm gruplardan kan örnekleri, karaciğer ve böbrek doku örnekleri alındı.
Bulgular: Total bilirübin, AST, ALT, kreatinin seviyeleri sarılıklı sıçanlarda anlamlı olarak yüksekti. 10. günde kontrol gruplarında AST, ALT, total bilirubin, BUN, kreatinin seviyeleri anlamlı olarak yüksekti. Melatonin, N–asetilsistein ve melatonin & N–asetilsistein grupları karşılaştırıldığında tüm biyokimyasal parametrelerin seviyelerinde anlamlı fark saptanmadı. Kontrol grubunda MDA ve NO seviyeleri çalışma grubundan daha yüksek saptandı. Melatonin, N–asetilsistein ve melatonin & N–asetilsistein grupları karşılaştırıldığında MDA ve NO seviyeleri açısından farklılık yoktu. Histopatolojik bulgularda MDA ve NO değerleri ile benzerlik gösteriyordu.
Sonuç: Tıkanma sarılıklı sıçanlarda melatonin ve NAC kullanılması karaciğer ve böbrek dokusunda serbest oksijen radikallerinin yarattığı hasardan korur.

Keywords

melatonin, n-asetilsistein, tıkanma sarılığı, serbest oskijen radikali

Project Number

TT–06–25

The Effects of Melatonin and N-Acetylcysteine on Obstructive Jaundiced Rats

Abstract

Aim: The aim of this experimental study is to investigate the effects of N-
acetylcysteine and melatoninin on the cholestasis and their protective effects on
liver and renal injury.
Materials and Methods: Forty eight rats were used in the study. Rats
were divided into three main groups as sham, main control, and study groups.
Main control group divided early sacrification group, control group, study group is divided
melatonin, N-acetylcysteine and melatonin & N-acetylcysteine groups. In study
and control groups, a laparotomy was performed and the common bile duct was
ligated and divided. Five days after the first operation, from early sacrification
group blood samples, and liver and renal tissues were collected. For study
groups from the fifth day to day 10 melatonin, N-acetylcysteine, melatonin &
N-acetylcysteine solutions were applied subcutaneous, saline to the sham and
control group. At the day ten from the all groups blood samples, and liver tissues
and renal tissues were collected.
Results: AST, ALT, BUN, creatinine, total bilirubin levels were
significantly higher in jaundiced rats than sham group rats. AST, ALT, total
bilirubin, BUN, creatinin levels were significantly higher in the control group
at the end of day 10. Among the melatonin group, N-acetylcyteine group
and melatonin & N-acetylcysteine group all biochemical parametres were not
different. Also the values of MDA and NO for control group were higher than
study groups. On the other hand, MDA and NO values were not different
between the melatonin group, N-acetylcyteine group and melatonin & N-
acetylcysteine group at the end study. Histopathologic findings were also
similiar as NO and MDA values.
Conclusion: Use of melatonin and N-acetylcysteine in obstructive jaundiced
rats, prevents damages of free oxygen radicals on liver and renal tissue.

Keywords

Melatonin, N-acetylcysteine,, obstructive jaundice, free oxigene radicals

Supporting Institution

Erciyes University

Project Number

TT–06–25

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