Investigation on Some Biological Activities of Different Parts of Vincetoxicum hirundinaria Medik.

Year 2023, Volume: 43 Issue: 2, 142 - 149, 01.06.2023

Zehra Öksüz , Sevda Güzel

https://doi.org/10.52794/hujpharm.1128462

Abstract

In this study, it was aimed to determine the antimicrobial, antibiofilm activities and synergistic effects of ethanol extracts obtained from above-ground, root, seed and seed coat of Vincetoxicum hirundinaria Medik. Antimicrobial activity was performed against 7 bacterial and 3 fungal reference strains by microdilution method and minimum inhibition concentrations (MIC) were determined. The crystal violet method was applied to determine the prevention of biofilm formation and inhibition of preformed biofilm activities on Pseudomonas aeruginosa biofilm. In addition, the synergistic effects of aerial part and seed extracts against both Escherichia coli and Enterococcus faecalis were examined by the microdilution checkerboard method. According to the antimicrobial results extracts had moderate to low efficacy against bacterial and yeast strains. The synergy test showed that the aerial and seed extracts had additive effect against both E.coli and E.faecalis. The extracts also showed the potential to inhibit biofilm formation and preformed biofilms. Especially root and seed pod extracts showed strong antibiofilm activity. In conclusion, the literature search indicated that the antimicrobial and antibiofilm activities of V. hirundinaria was evaluated for the first time in the current study, therefore; our findings provide important preliminary data to the literature in terms of antibiofilm activity of V. hirundinaria.

Keywords

Antimicrobial, Antibiofilm, Synergy Test, V. hirundinaria

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