Ticari Zerdeçal Kapsüllerinde Kurkumin Tayini için RP-HPLC Yöntemi Geliştirme

Year 2024, Volume: 44 Issue: 1, 1 - 8, 01.03.2024

Duygu Eneş , Mustafa Çelebier , Derya Dikmen , Sacide Altınöz

https://doi.org/10.52794/hujpharm.1253858

Cited By: 2

Abstract

Curcumin is a natural polyphenol product isolated from the rhizome of the Curcuma longa plant and is the most active ingredient of this plant. Curcumin is hepatoprotective, nephroprotective, anti-thrombotic, cardioprotective, anti-diabetic and anti-rheumatic. The aim of this study was to develop an HPLC method for the analysis of curcumin in commercially available capsules. Supelco Discovery 5 µm C18 Column (4.6mm x 5.0 µm) was used for chromatographic separation. The mobile phase was acetonitrile (ACN), 20 mM phosphate buffer, pH 6 (40:60 v/v). The flow rate was 1.5 mL min-1. UV detection was at 262 nm. The retention time was about 6.13th minutes for curcumin. The developed method has been validated according to ICH guidelines. The method was found to be selective under the experimental conditionsIt was linear over the range 0.05 - 50.0 μg mL-1. The intraday and interday studies showed that the RSD% was less than 2.0 for intraday and 2.0 for interday, and the Bias% was less than 2.0 for intraday and 2.0 for interday. The method could be successfully used in curcumin-loaded commercial capsules' quality control.

Keywords

Kurkumin, Zerdeçal, HPLC, Validasyon, Tayin

RP-HPLC Method Development for Determination of Curcumin in Commercial Turmeric Capsules

Abstract

Curcumin is a natural polyphenol product isolated from the rhizome of the Curcuma longa plant and is the most active ingredient of this plant. Curcumin is hepatoprotective, nephroprotective, anti-thrombotic, cardioprotective, anti-diabetic and anti-rheumatic. The aim of this study was to develop an HPLC method for the analysis of curcumin in commercially available capsules. Supelco Discovery 5 µm C18 Column (4.6mm x 5.0 µm) was used for chromatographic separation. The mobile phase was acetonitrile (ACN), 20 mM phosphate buffer, pH 6 (40:60 v/v). The flow rate was 1.5 mL min-1. UV detection was at 262 nm. The retention time was about 6.13th minutes for curcumin. The developed method has been validated according to ICH guidelines. The method was found to be selective under the experimental conditionsIt was linear over the range 0.05 - 50.0 μg mL-1. The intraday and interday studies showed that the RSD% was less than 2.0 for intraday and 2.0 for interday, and the Bias% was less than 2.0 for intraday and 2.0 for interday. The method could be successfully used in curcumin-loaded commercial capsules' quality control.

Keywords

Curcumin, Turmeric, HPLC, Validation, Determination

References

• 1. Zhou H, Beevers CS and Huang S. The targets of curcumin. Curr Drug Targets. 2011;12(3):332-347. https://doi.org/10.2174/138945011794815356
• 2. Shishodia S, Sethi G and Aggarwal BB. Curcumin: getting back to the roots. Ann N Y Acad Sci. 2005;1056(1):206-217. https://doi.org/10.1196/annals.1352.010
• 3. Indira Priyadarsini K. Chemical and structural features influencing the biological activity of curcumin. Curr Pharm Des. 2013;19(11):2093-2100. https://doi.org/10.2174/138161213805289228
• 4. Slika L and Patra D. A short review on chemical properties, stability and nano-technological advances for curcumin delivery. Expert Opin Drug Deliv. 2020;17(1):61-75. https://doi.org/10.1080/17425247.2020.1702644
• 5. Pari L, Tewas D and Eckel J. Role of curcumin in health and disease. Arch Physiol Biochem. 2008;114(2):127-149. https://doi.org/10.1080/13813450802033958
• 6. Pulido-Moran M, et al. Curcumin and health. Molecules. 2016;21(3):264. https://doi.org/10.3390/molecules21030264
• 7. Wakte PS, et al. Optimisation of microwave, ultra-sonic and supercritical carbon dioxide assisted extraction techniques for curcumin from Curcuma longa. Sep Purif Technol. 2011;79(1):50-55. https://doi.org/10.1016/j.seppur.2011.03.010
• 8. Banu KS and Cathrine L. General techniques involved in phytochemical analysis. IJARCS. 2015;2(4):25-32.
• 9. Yadav R and Agarwala M. Phytochemical analysis of some medicinal plants. J Phytol. 2011;3(12).
• 10. Singh S and Aggarwal BB. Activation of transcription factor NF-κB is suppressed by curcumin (diferuloylmethane)(∗). J Biol Chem. 1995;270(42):24995-25000. https://doi.org/10.1074/jbc.270.42.24995.
• 11. Liang G, et al. Inhibition of LPS-induced production of inflammatory factors in the macrophages by mono-carbonyl analogues of curcumin. J Cell Mol Med. 2009;13(9b):3370-3379. https://doi.org/10.1111/j.1582-4934.2009.00711.x
• 12. Chen A and Xu J. Activation of PPARγ by curcumin inhibits Moser cell growth and mediates suppression of gene expression of cyclin D1 and EGFR. Am J Physiol Gastrointest and Liver Physiol. 2005;288(3):G447-G456. https://doi.org/10.1152/ajpgi.00209.2004
• 13. Durgaprasad S, Pai CG and Alvres JF. A pilot study of the antioxidant effect of curcumin in tropical pancreatitis. Indian J Med Res. 2005;122(4):315.
• 14. Hanai H, et al. Curcumin maintenance therapy for ulcerative colitis: randomised, multicenter, double-blind, placebo-controlled trial. Clin Gastroenterol Hepatol. 2006;4(12):1502-1506. https://doi.org/10.1016/j.cgh.2006.08.008
• 15. Phan TT, et al. Protective effects of curcumin against oxidative damage on skin cells in vitro: its implication for wound healing. J Trauma. 2001;51(5):927-931. https://doi.org/10.1097/00005373-200111000-00017
• 16. Aggarwal BB, et al. Curcumin: the Indian solid gold. Adv Exp Med Biol. 2007;595:1-75. https://doi.org/10.1007/978-0-387-46401-5_1
• 17. Hatcher H, et al. Curcumin: from ancient medicine to current clinical trials. Cell Mol Life Sci. 2008, 65(11):1631-1652. https://doi.org/10.1007/s00018-008-7452-4
• 18. Jagetia GC and Aggarwal BB. “Spicing up” of the immune system by curcumin. J Clin Immunol. 2007, 27(1):19-35. https://doi.org/10.1007/s10875-006-9066-7
• 19. Anand P, et al. Bioavailability of curcumin: problems and promises. Mol Pharm. 2007;4(6):807-818. https://doi.org/10.1021/mp700113r
• 20. Kocaadam B and Şanlier N. Curcumin, an active component of turmeric (Curcuma longa), and its effects on health. Crit Rev Dood Sci and Nut. 2017;57(13):2889-2895. https://doi.org/10.1080/10408398.2015.1077195
• 21. Sri SN, et al. Curcumin-based food supplements: Challenges and future prospects. Nonvitamin and Nonmineral Nutritional Supplements. 2019;119-128. https://doi.org/10.1016/B978-0-12-812491-8.00016-3
• 22. Goel A, Kunnumakkara AB and Aggarwal BB. Curcumin as “Curecumin”: from kitchen to clinic. Biochem Pharmacol. 2008;75(4):787-809. https://doi.org/10.1016/j.bcp.2007.08.016
• 23. Maughan RJ. Quality assurance issues in the use of dietary supplements, with special reference to protein supplements. J Nutr. 2013;143(11):1843S-1847S. https://doi.org/10.3945/jn.113.176651
• 24. Sanzini E, et al. Quality control of plant food supplements. Food Funct. 2011;2(12):740-746. https://doi.org/10.1039/c1fo10112a
• 25. Jayaprakasha GK, Jagan Mohan Rao L and Sakariah KK. Improved HPLC method for the determination of curcumin, demethoxycurcumin, and bisdemethoxycurcumin. J Agric Food Chem. 2002;50(13):3668-3672. https://doi.org/10.1021/jf025506a
• 26. Jadhav BK, Mahadik KR and Paradkar AR. Development and validation of improved reversed phase-HPLC method for simultaneous determination of curcumin, demethoxycurcumin and bis-demethoxycurcumin. Chromatographia. 2007;65:483-488. https://doi.org/10.1365/s10337-006-0164-8
• 27. Kadam PV, et al. Standardisation and quantification of curcumin from Curcuma longa extract using U.V. visible spectroscopy and HPLC. J Pharmacogn Phytochem. 2018;7(5):1913-1918.
• 28. Guideline IHT. Validation of analytical procedures Q2 (R1). in International conference of harmonisation. Geneva, Switzerland. 2022.