Prediyabetik Gebelerde 50 gr Glukoz Yükleme Testinin Oksidatif Stres ve İnterlökin-8 Parametreleri Üzerine Etkisinin Değerlendirilmesi

Year 2022, , 510 - 514, 27.12.2022

Zeliha Cansel Özmen , Cuma Mertoğlu , Leyla Aydoğan , Mehmet Can Nacar , Köksal Deveci , Muzaffer Katar , Zeki Özsoy

https://doi.org/10.35440/hutfd.1082328

Abstract

Amaç: Gestasyonel diyabetes mellitus (GDM), gebelikte β-hücre fonksiyon bozukluğu ve insülin direncinin metabolik defektleri ile karakterize, gebeliğin yaygın bir tıbbi komplikasyonudur. Bu çalışmada, prediabetik gebelerde 50 gr oral glukoz tolerans testi (OGTT)’nin oksidatif stres ve interlökin-8 (IL-8) parametreleri üzerine etkisinin değerlendirilmesi amaçlanmıştır.
Gereç ve Yöntemler: 50 gr OGTT yapılan 79 gebeden açlık ve 1. saat kan numuneleri alındı. Birinci saat kan şekeri 140– 200 mg/dl olan hastalar prediyabetik grup olarak kabul edildi. Daha sonra açlık ve 1. saatte alınan serum numunelerinde, rutin biyokimya parametrelerinin ve süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), malondialdehit (MDA) ve interlökin-8 (IL-8) parametrelerinin düzeyleri ölçüldü.
Bulgular: Prediabetik grup serum GPx ve SOD düzeyleri, kontrol grubundan anlamlı derecede düşük bulundu (p<0,05). Prediabetik grup serum IL-8 düzeyleri ise kontrol grubundan anlamlı olarak yüksek bulundu (p<0,05). OGTT sırasında parametrelerin açlık ve 1. saat düzeyleri karşılaştırıldığında ise SOD düzeylerinde anlamlı derecede azalma (p<0,001) ve IL-8 düzeylerinde ise anlamlı olarak yükseklik bulundu (p<0,001).
Sonuç: Sonuç olarak prediabetik gebelerde serum GPX ve SOD düzeylerinde azalma, serum IL-8 düzeylerinde ise artış olduğu, bununla birlikte bu hastalara 50 gr OGTT yapılması durumunda da SOD düzeylerinde azalma ve IL-8 düzeylerinde ise artış olduğu bulundu. Bulduğumuz bu sonuçlar bize prediabetik gebelerde zaten var olan oksidatif stres ve sistemik inflamasyonun 50 gr OGTT ile daha da tetiklenerek gebe için olumsuz risk faktörleri oluşturabileceğini düşündürdü.

Keywords

inflamasyon, OGTT, oksidatif stres, prediyabet

Supporting Institution

Çalışma Tokat Gaziosmanpaşa Üniversitesi Bilimsel Araştırma ve Projeler Birimi (2015/111) tarafından desteklenmiştir.

Project Number

2015/111

Evaluation the Effect of 50 g Glucose Tolerance Test on Oxidative Stress and Interleukin-8 Parameters in Prediabetic Pregnancy

Abstract

Background: The aim of this study is to evaluate the effect of 50 g oral glucose tolerance test (OGTT) on oxidative stress and interleukin-8 (IL-8) parameters in prediabetic pregnant women.
Materials and Methods: Fasting and 1 hour blood samples were collected from 79 pregnant women who were administered 50 gr OGTT. Patients with a 1 hour blood glucose level of 140–200 mg/dl were considered as the prediabetic group. Thereafter, routine biochemical parameters and the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA) and interleukin-8 (IL-8) parameters were measured from the serum samples taken during fasting and at 1 hour.
Results: The serum GPx and SOD levels of the prediabetic group were remarkably lower than that of the control group (p<0.05). Whereas the serum IL-8 levels of the prediabetic group were significantly higher than that of the control group (p<0.05). When the fasting and 1 hour levels of the parameters were compared during OGTT, SOD levels were significantly decreased (p<0.001) and IL-8 levels were significantly higher (p<0.001).
Conclusions: In conclusion, it was found that serum GPX and SOD levels decreased, while serum IL-8 levels increased in prediabetic pregnant women; however, when 50 g OGTT was administered to these patients, SOD levels decreased and IL-8 levels increased. These results we obtained suggest that oxidative stress and systemic inflammation that are already present in prediabetic pregnant women may be triggered by 50 g OGTT, posing negative risk factors for pregnant women.

Keywords

inflammation, OGTT, oxidative stress, prediabetes

Project Number

2015/111

References

• REFERENCES 1. Agarwal A, Hegde A, Yadav C, Ahmad A, Manjrekar PA, Srikantiah RM. Assessment of oxidative stress and inflammation in prediabetes-A hospital based cross-sectional study. Diabetes Metab Syndr 2016; 10(2): 123-6.
• 2. Korkmazer E, Solak N. Correlation between inflammatory markers and insulin resistance in pregnancy. J Obstet Gynaecol 2015; 35(2): 142-5.
• 3. Paradısı G, Bıaggı A, Ferrazzanı S, Carolis S, Caruso A. Abnormal Carbohydrate Metabolism During Pregnancy: association with endothelial dysfunction. Diabetes Care 2002; 25(3): 560-4.
• 4. Punthumapol C, Tekasakul P. 50 Grams Glucose Challenge Test for Screening of Gestational Diabetes Mellitus in Each Trimester in Potential Diabetic Pregnancy. J Med Assoc Thai 2008; 91(6): 787-93.
• 5. Yee LM, Cheng YW, Liddel J, Block-Kurbisch I, Caughey AB. 50-Gram glucose challenge test: is it indicative of outcomes in women without gestational diabetes mellitus? J Matern Fetal Neonatal Med 2011; 24(9): 1102-6.
• 6. Wang Y, Tan M, Huang Z, Sheng L, Ge Y, Zhang H et al. Elemental contents in serum of pregnant women with gestational diabetes mellitus. Biol Trace Elem Res 2002; 88(2): 113-8.
• 7. Akhlaghi F, Bagheri SM, Rajabi O. A comparative study of relationship between micronutrients and gestational diabetes. ISRN Obstet Gynecol 2012; 2012: 470419.
• 8. Clapes S, Fernandez T, Suarez G. Oxidative stress and birth defects in infants of women with pregesstational diabetes. MEDICC Rev 2013; 15(1): 37-40.
• 9. López-Tinoco C, Roca M, García-Valero A, Murri M, Tinahones FJ, Segundo C et al. Oxidative stress and antioxidant status in patients with late-onset gestational diabetes mellitus. Acta Diabetol 2013; 50(2): 201-8.
• 10. Kızıler A.R, Aydemir B, Cinemre F.B, Cinemre H, Gülyaşar T, Tüten A et al. Relationships Among Some Biochemical Parameters, Trace Elements and Lipid Peroxidation Levels in Women With Gestational Diabetes Mellitus. Int J Basic Clin Med 2013; 1(3): 157-64.
• 11. Sudharshana Murthy KA, Bhandiwada A, Chandan SL, Gowda SL, Sindhusree G. Evaluation of Oxidative Stress and Proinflammatory Cytokines in Gestational Diabetes Mellitus and Their Correlation with Pregnancy Outcome. Indian J Endocrinol Metab 2018; 22(1): 79-84.
• 12. Gordin D, Forsblom C, Rönnback M, Parkkonen M, Wadén J, Hietala K et al. Acute hyperglycaemia induces an inflammatory response in young patients with type 1 diabetes. Ann Med 2008; 40(8): 627-33.
• 13. Manning P.J, Sutherland W.H, Walker R.J, Jong S, Berry E.A. The effect of glucose ingestion on inflammation and oxidative stress in obese individuals. Metabolism 2008; 57(10): 1345-9.
• 14. Esposito K, Nappo F, Marfella R, Giugliano G, Giugliano F, Ciotola M et al. Inflammatory cytokine concentrations are acutely increased by hyperglycemia in humans: role of oxidative stress. Circulation 2002; 106(16): 2067-72.
• 15. Ceriello A, Assaloni R, Da Ros R, Maier A, Piconi L, Quagliaro Let al. Effect of atorvastatin and irbesartan, alone and in combination, on postprandial endothelial dysfunction, oxidative stress, and inflammation in type 2 diabetic patients. Circulation 2005; 111(19): 2518-24.
• 16. Dhindsa S, Tripathy D, Mohanty P, Ghanim H, Syed T, Aljada A et al. Differential effects of glucose and alcohol on reactive oxygen species generation and intranuclear factor–κB in mononuclear cells. Metabolism 2004; 53(3): 330-4.
• 17. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34(3): 497-500. 18. Paglia D. E, Valentine W. N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clinical Med 1967; 70(1): 158-69.
• 19. Esterbauer H, Cheeseman K. H. Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal. Methods Enzymol 1990; 186: 407-21.
• 20. Donma M, Donma O, Topçu B, Aydın M, Tülübaş F, Nalbantoğlu B et al. A New Insulin Sensitivity Index Derived From Fat Mass Index and Quantitative Insulin Sensitivity Check. Index Int J Basic Clin Med 2015; 3(1): 26-36.
• 21. Derosa G, D'Angelo A, Salvadeo SA, Ferrari I, Fogari E, Gravina A et al. Oral Glucose Tolerance Test Effects on Endothelial Inflammation Markers in Healthy Subjects and Diabetic Patients. Horm Metab Res 2010; 42(1): 8-13.
• 22. Ates I, Kaplan M, Inan B, Alişik M, Erel O, Yılmaz N et al. How does thiol/disulfide homeostasis change in prediabetic patients? Diabetes Res Clin Pract 2015; 110(2): 166-71.
• 23. Peuchant E, Brun JL, Rigalleau V, Dubourg L, Thomas MJ, Daniel JY et al. Oxidative and antioxidative status in pregnant women with either gestational or type 1 diabetes. Clin Biochem. 2004;37(4):293-8.
• 24. Chaudhari L, Tandon OP, Vaney N, Agarwal N. Lipid peroxidation and antioxidant enzymes in gestational diabetics. Indian J Physiol Pharmacol 2003; 47(4): 441-6.
• 25. Rajdl D, Racek J, Steinerova A, Novotny Z, Stozicky F, Trefil L. Markers of oxidative stress in diabetic mothers and their infants during delivery. Physiol Res 2005; 54(4): 429-36.
• 26. Lappas M, Permezel M, Rice GE. Release of Proinflammatory Cytokines and 8-Isoprostane from Placenta, Adipose Tissue, and Skeletal Muscle from Normal Pregnant Women and Women with Gestational Diabetes Mellitus. J Clin Endocrinol Metab 2004; 89(11): 5627-33.
• 27. Rueangdetnarong H, Sekararithi R, Jaiwongkam T, Kumfu S, Chattipakorn N, Tongsong T et al. Comparisons of the oxidative stress biomarkers levels in gestational diabetes mellitus (GDM) and non-GDM among Thai population: cohort study. Endocr Connect 2018; 7(5): 681-7.
• 28. Vrachnis N, Belitsos P, Sifakis S, Dafopoulos K, Siristatidis C, Pappa KI, et al. Role of adipokines and other inflammatory mediators in gestational diabetes mellitus and previous gestational diabetes mellitus. Int J Endocrinol 2012; 2012: 549748
• 29. Straczkowski M, Kowalska I, Nikolajuk A, Dzienis-Straczkowska S, Szelachowska M, Kinalska I. Plasma interleukin 8 concentrations in obese subjects with impaired glucose tolerance. Cardiovasc Diabetol 2003; 2: 5
• 30. Urakaze M, Temaru R, Satou A, Yamazaki K, Hamazaki T, Kobayashi M. The 1L-8 Production in Endothelial Cells is Stimulated by High Glucose. Horm Metab Res 1996; 28(8): 400-1.
• 31. Choi HJ, Jeon SY, Hong WK, Jung SE, Kang HJ, Kim JW et al. Effect of glucose ingestion in plasma markers of inflammation and oxidative stress: Analysis of 16 plasma markers from oral glucose tolerance test samples of normal and diabetic patients. Diabetes Res Clin Pract 2013; 99(2): 27-31.