Investigation of DNA Damage in Rats in Chronically Exposed to Sub-Hypnotic Dose Desflurane

Year 2015, Volume: 12 Issue: 1, 44 - 52, 15.04.2015

Nuray Altay Zeynep Baysal Yıldırım

Abstract

Background: In this study the aim was to research the DNA damage in rats whose chronic desfluran gases
exposed.
Metods: This experimental study, after the Ethic Committe permission was made in Harran University
Medical Faculty Farmacology Department. For the study 200-230 gr 18 female albino wistar rats were used.
After and before the study care and the feeding of the rats were made in the Harran University experimental
animal laboratory. Rats divided in two groups (Group I: Desfluran, Grup II: Control ). For the anaesthesia
pump system we used special planned plastic plate. Datex Ohmeda Tec 6 plus vaporizator was used to Group
I rats to whom hypnotic doses desflurane was given ( % 0,5-1 ). İn the second group same mechanism was
used but the desflurane vaporizator was closed. Each group %50 O2 and desflurane gas mixture was given in
the 5lt/minute speed. In the environment the desflurane and the O2 concentration were measured continously
by the anaesthesia gases monitor ( criticare ). The condition of exposing to the gases, again 1 hour in 5 days.
Outside of experiment, rats waited in room condition and in their cages. At the end of fifth day, 50mg/kg with
penthotal sodium, general anaesthesia was made and their extremities were fixed in the supine position fixing
to the table. Blood samples were taken from intracardiac undertaking. The blood samples were taken
heparinized tubes and put in cold condition in the special plate. Then sent to the Harran University Medical
Faculty Biochemical Department. The experimental rats sacrificed and then burried with lime. DNAdamage
was measured in taken bloods mononuclear leukocytes with Comet Assay method. İn addition, in same blood
samples were measured antioxidant capacity and oxidative stress.
Results: During the study, general anaesthesia conditions in any rat were not seen and rats were continued
their normal activity. DNAdamage, antioxidant capacity and oxidative stress were similar in both groups.
Conclusions: It was concluded that not occur in a significant DNA damage, compared to the control
group in rats exposed chronically desflurane.

Keywords

Desflurane, chronic exposure, DNAdamage

Sıçanlarda, Subhipnotik Doz Kronik Desfluran Maruziyetinde, DNA Hasarının Araştırılması

Abstract

Amaç: Bu çalışmada, subhipnotik dozda, kronik desfluran gazına maruz kalan sıçanlardaki DNA hasarının
araştırılması amaçlandı.
Materyal ve metod: Deneysel çalışma, Harran Üniversitesi Tıp Fakültesi Etik Kurulu izni sonrasında aynı
fakültenin Farmakoloji Laboratuarında yapıldı. Çalışma için 200-230gr ağırlığında 18 adet dişi Wistar türü
albino sıçan kullanıldı. Sıçanların çalışma öncesi ve sonrasındaki beslenme ve bakımları, Harran
Üniversitesi Deney Hayvanları Laboratuarında yapıldı. Sıçanlar randomize 2 gruba ayrıldı (Grup
I=Desfluran, Grup II= Kontrol). Anestezik gaz verme sistemi için, özel olarak tasarlanmış plastik kaplar
kullanıldı. Grup I' de sıçanlara, Datex-Ohmeda Tec6 Plus vaporizatörü ile hipnoz oluşturmayacak dozlarda
(%0.5-1) desfluran verildi. Grup II'de de aynı düzenek kullanıldı, ancak desfluran vaporizatörü kapalı
tutuldu. Her iki grupta deney düzeneğine %50 oksijen-hava karışımı 5 litre /dakika taze gaz akışı hızında
verildi. Ortamdaki desfluran ve oksijen konsantrasyonları bir anestezik gaz monitörü (Criticare ®) ile sürekli
olarak ölçüldü. Çalışma gazlarına maruz kalma durumu 5 gün boyunca günde 1 saat olacak şekilde
tekrarlandı. Deney dışındaki saatlerde sıçanlar kendi kafeslerinde oda şartlarında bakıldılar. Beşinci günün
sonunda 50 mg/kg intraperitoneal pentothal ile anestezisi sağlanan sıçanlar, supin pozisyonunda
ekstremitelerden masaya tespit edildi. Sıçanlardan intrakardiak girişimle kan örnekleri alındı. Alınan kanlar
heparinli tüplere aktarılarak özel bir kap içerisinde, soğuk ortamda, en kısa sürede Harran Üniversitesi Tıp
Fakültesi Biyokimya Anabilim Dalı Laboratuarına ulaştırıldı. Deney hayvanları kan alındıktan sonra
sakrifiye edilip, üzerlerine kireç kaymağı dökülüp toprağa gömüldüler. Alınan kanlardaki mononükleer
lökositlerinden Comet Assey yöntemi ile DNAhasarları ölçüldü. Ayrıca aynı kan örneklerinden, antioksidan
kapasite ve oksidatif stress indeks değerleri analiz edildi.
Bulgular: Deneysel çalışma sırasında hiçbir sıçanda genel anestezi durumu oluşmazken, tüm sıçanlar 5 gün
boyunca normal aktivitelerini sürdürdüler. Kan örneklerinden çalışılan DNAhasarı, antioksidan kapasite ve
oksidatif stres indeks değerleri, her iki grupta benzer bulundu.

Sonuç: Kronik olarak desflurana maruz kalan sıçanlarda, kontrol grubuna göre, belirgin bir DNA hasarının
oluşmadığı sonucuna varıldı.

Keywords

Desfluran, kronik maruziyet, DNAhasarı

References

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