The Protective Effect of Vitamin B12 on Epileptic Seizure Activity in Rats Induced by Penicillin

Şeyma Özsoy; Ziya Çakır; Zeynep Kasap Acungil

doi:10.35440/hutfd.1456429

Research Article

Penisilin ile İndüklenen Sıçanlardaki Epileptik Nöbet Aktivitesinde B12 Vitamininin Koruyucu Etkisi

Year 2024, Volume: 21 Issue: 2, 194 - 200, 29.08.2024

Şeyma Özsoy , Ziya Çakır , Zeynep Kasap Acungil

https://doi.org/10.35440/hutfd.1456429

Abstract

Amaç: Merkezi sinir sisteminde ani, anormal ve kontrolsüz nöronal aktivite ile karakterize edilen epilepsi, kronik nörolojik bir hastalıktır. B12 vitamini türevleri, belirli bir grup enzim için karmaşık organometalik yardımcılar olarak görev yapar. Bu vitamin merkezi ve periferik sinir sistemindeki birçok hücresel fonksiyonu etkiler. Bu çalışma, B12 Vitamininin anestezi altındaki sıçanlarda penisilin kaynaklı epileptik benzeri aktiviteyi elektrokortikografi (ECoG) yoluyla nasıl etkilediğini araştırmayı amaçladı.
Materyal ve Metod: Bu çalışma için 35 adet erkek Wistar sıçanı kullanıldı ve her biri 7 sıçandan oluşan beş gruba ayrıldı. Gruplar şu şekildeydi: Kontrol +Penisilin; Sıçanlara intrakraniyal (i.c.) olarak 2.5 μL 500 IU penisilin ve intraperitoneal (i.p.) olarak 1 ml salin solüsyonu verildi, 1 mg/kg Vit B12 +Penisilin; Sıçanlara intraperitoneal (i.p.) olarak 1 mg/kg Vitamin B12 ve penisilin verildi, 2 mg/kg Vit B12 +Penisilin; Sıçanlara intraperitoneal (i.p.) olarak 2 mg/kg Vitamin B12 ve penisilin verildi, VPA +Penisilin; Sıçanlara intraperitoneal (i.p.) olarak 500 mg/kg sodyum valproat ve penisilin verildi, ve 2 mg/kg Vit B12 + VPA +Penisilin; Sıçanlara 2 mg/kg Vitamin B12 i.p. ve 500 mg/kg sodyum valproat i.p. ve penisilin verildi, ilaçlar penisilinden 30 dakika sonra uygulandı. Uygulama sonrasında stereotaksik cihaza yerleştirilen sıçanlardan (180 dakika süreyle) ECoG kayıtları alındı.
Bulgular: B12 Vitamininin hemen uygulanması, sıçanlarda penisilin kaynaklı epileptiform aktivitenin hem spike-dalga frekansında hem de spike-dalga amplitüdünde anlamlı bir azalmaya neden oldu (p<0.05).
Sonuç: Bu sonuçlar, B12 Vitamininin akut olarak uygulamasının penisilin kaynaklı fokal başlangıçlı epileptik aktivite üzerinde antikonvülsan etkiler gösterdiğine işaret etmektedir. Çalışma ayrıca B12 vitamini tedavisinin anti-epileptojenik potansiyele sahip olabileceğini öne sürüyor.

Keywords

Epilepsi, B12 Vitamini, Penisilin.

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