Investigation of PLGF, sFlt-1 Expression in Placenta Previa, Placenta Accreta and Normotensive Placentas in the 3rd Trimester of Pregnancy

Year 2024, Volume: 21 Issue: 3, 407 - 414

Gamze Erdoğan , Seval Kaya , Nurullah Peker , İbrahim İbiloğlu , Engin Deveci

https://doi.org/10.35440/hutfd.1487194

Abstract

Background: We aimed to immunohistochemically examine the expression level of Placental growth factor (PLGF) and Soluble FMS-like tyrosine kinase-1 (sFlt-1) proteins in normotensive, Placenta accreta (PA) and Placenta previa (PP).
Materials and Methods: Three groups were created for the study: 20 Nomotensive placentas, 20 PP and 20 PA from women diagnosed with placenta samples. 4-6 μm thick sections were taken from the placentas, PLGF and sFlt-1 immunostaining were applied to the obtained sections, and their expression intensities and localizations in the tissues were determined.
Results: As a result of our observations, normotensive placenta group; sFlt-1 expression was observed in hofbauer cells and syncytial nodes and PLGF positive expression was observed in nuclei of muscle cells in some tunica media region. PP and PA group; sFlt-1 expression was positive in decidua cells, hofbauer cells and dilated vascular endothelium. PP and PA group; negative PLGF expression was observed in syncytial nodes and positive PLGF expression was observed in hofbauer cells and endothelial cells. There was a significant difference between normotensive, PP and PA groups (p<0.05).
Conclusions: It can be thought that sFlt-1 and PLGF may be important signal stimulators and markers in the trophoblastic degeneration, apoptotic cells, increase in angiogenesis and imbalance in implanta-tion seen in cases of PP and PA.

Keywords

Placenta accreta, Placenta previa, PLGF, sFlt-1

Project Number

TIP.23.003

Gebeliğin 3. Trimestrinde Plasenta Previa, Plasenta Akreata ve Normotensif Plasentalarda PLGF, sFlt-1 ekspresyonunun incelenmesi

Abstract

Amaç: Normotensif, Plasenta akreata (PA) ve Plasenta previa (PP); Plasenta büyüme faktör (PLGF) ve Soluble Fms-Benzeri Tirozin Kinaz-1 (sFlt-1) proteinlerin ekspresyon düzeylerinin immünohistokimyasal olarak incelenmesini amaçladık.
Materyal ve Metod: Çalışmaya 20 Nomotensif plasenta, 20 PP ve 20 PA tanısı konmuş kadına ait plasen-ta örnekleri olmak üzere 3 grup oluşturulmuştur. Plasentalardan 4-6 μm kalınlığında kesitler alındı, elde edilen kesitlere PLGF ve sFlt-1 immün boyama uygulanarak dokulardaki ekspresyon şiddetleri ve loka-lizasyonları belirlendi.
Bulgular: Gözlemlerimiz sonucu normotensif plasenta grubu; hofbauer hücreler ve sinsityal nodlar sFlt-1 ve bazı tunika media bölgesindeki kas hücrelerin nukleuslarında PLGF pozitif ekspresyonu izlendi. PP ve PA grubu; desidua hücreler, hofbauer hücrelerinde ve dilate damar endotelinde sFlt-1 ekspresyonu pozitif izlendi. PP ve PA grubu; sinsityal düğümlerde negatif, hofbauer hücrelerinde ve endotel hücre-lerinde pozitif PLGF ekspresyonu izlendi. Normotensif, PP ve PA grupları arasında anlamlı bir fark vardı (p<0.05).
Sonuç: PP ve PA olgularında görülen trofoblastik dejenerasyon ve apoptotik hücreler, anjiyogenezisin artmasına ve implantasyonda dengesizliğin gerçekleşmesinde sFlt-1 ve PLGF etkili olabileceğini düşün-mekteyiz.

Keywords

Plasenta akreata, Plasenta previa, PLGF, sFlt-1

Ethical Statement

Araştırma, Dicle Üniversitesi Tıp Fakültesi Etik Kurulu'nun 17.03.2022 tarih ve 67 sayılı onayı ile başlatılmıştır.

Supporting Institution

Dicle Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı

Project Number

TIP.23.003

Thanks

Danışman Hocam Prof.Dr. Engin DEVECİ 'ye Desteklerinden dolayı teşekkürlerimi sunarım.

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