Aydın ve İzmir İllerindeki Koyun ve Keçilerde Pestivirus Enfeksiyonunun Serolojik ve Virolojik Olarak Araştırılması

Year 2017, Volume: 6 Issue: 1, 63 - 68, 30.06.2017

Zafer Emin Ural Nural Erol

https://doi.org/10.31196/huvfd.325757

Cited By: 3

Abstract

Bu çalışma ile Aydın ve İzmir
illerindeki koyun ve keçilerde pestivirus enfeksiyonunun serolojik ve virolojik
olarak araştırılması amaçlanmıştır. Çalışmada 128 adet keçi, 332 adet koyun
olmak üzere toplam 460 adet hayvandan serum örnekleri elde edildi. Alınan kan
serumu örneklerinde, bovine viral diarrhea virusunun (BVDV) referans suşu NADL
kullanılarak virus nötralizasyon testi ile antikor varlığı araştırıldı. Toplam
348 adet (116 adet keçi, 232 adet koyun) hayvandan alınan tam kan örnekleri
pestivirus spesifik antijen varlığı yönünden ticari Enzyme Linked Immunosorbent
Assay (ELISA) (Serelisa^® BVD p80Ag Mono
Indirect, Synbiotics, Europe, Lyon, France) ile test edildi. Keçi örneklerinin
%41.40’ının (53 /128), koyun örneklerinin ise %47.59’unun (158/332) seropozitif
olduğu saptandı. Seropozitif bulunan toplam 211 serum örneğinin
%37.44’ünde 1/5, %34.12’sinde 1/10, %14.21’inde 1/20, %5.21’inde 1/40, %5.68’inde
1/80 ve %3.31’inde ise 1/160 olarak SN₅₀ değerleri belirlendi. Test edilen tam kan
örneklerinin antijen yönünden negatif olduğu belirlendi. Bu çalışmada, Aydın ve
İzmir illerinde örneklenen koyun ve keçilerde viremik hayvan tespit edilmemiş
olmasına rağmen, %45.87 (211/460) oranında seropozitiflik saptanması
pestivirusların bölgede sirküle olduğunu ve önemli ekonomik kayıplara neden
olabileceğini göstermektedir.

Keywords

Antijen, Antikor, Keçi, Koyun, Pestivirus

A Serological and Virological Investigation of the Pestivirus Infections in Sheep and Goats in the Aydin and Izmir Provinces

Abstract

The objective
of this study was serological and virological investigation of pestivirus
infections in sheep and goats in the Aydin and Izmir provinces. In the study,
460 serum samples were collected from 128 goats and 332 sheep. The presence of
antibodies in the blood serum samples was investigated using NADL, the
reference strain for bovine viral diarrhea virus (BVDV) in virus neutralization
test. In total, 348 whole blood samples collected from 116 goats and 232 sheep
were tested for the presence of the pestivirus specific antigens using
commercially available enzyme linked immunosorbent assay (ELISA) (Serelisa® BVD
p80ag Mono Indirect, Synbiotics, Europe, Lyon, France). 41.40% (53/128) of the
goat and 47.59% (158/332) of the sheep samples were found to be seropositive.
SN₅₀ values were 1/5 in 37.44%, 1/10 in 34.12%, 1/20 in 14.21%, 1/40
in 5.21%, 1/80 in 5.68% and 1/160 in 3.31% of the 221 seropositive serum
samples. The whole blood samples tested were negative for the antigen. Although
no viremia was detected in any of the sheep and goat tested in this study, the
45.87% (211/460) rate of seropositivity shows that Pestiviruses may be
circulating in the region and may cause significant economic losses.

Keywords

Pestivirus, Antigen, Antibody, Goat, Sheep.

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