Türkiye’de Yetiştirilen Et Irkı Kültür Sığırlarında Leptin, Ghrelin ve İnsülin Benzeri Büyüme Faktörü -1 (IGF-1) Gen Polimorfizmlerinin Belirlenmesi

Year 2019, Volume: 8 Issue: 1, 108 - 115, 01.07.2019

Aydın Daş Tekin Şahin Ömer Akbulut A. Şükrü Bengü Faruk Bozkaya

https://doi.org/10.31196/huvfd.592643

Cited By: 2

Abstract

Bu
çalışma, Türkiye’de yetiştirilen et ırkı kültür sığırlarında leptin, ghrelin ve
insülin benzeri büyüme faktörü -1 (IGF-1) geni polimorfizmlerinin belirlenmesi,
polimorfizmler yönünden genotip ve allel sıklıklarının tespit edilmesi amacıyla
yapılmıştır. Çalışmanın materyalini Şanlıurfa Harranova Besi ve Tarım
İşletmesi’nde bulunan ve fenotipik değerlendirmeyle seçilmiş Hereford (n=112),
Angus (n=145), Şarole (n=54), 36 Siyah Hereford (n=36), Brahman (n=24) ve
Limousin (n=34) ırkı toplam 405 baş erkek hayvan oluşturmuştur. Hayvanların et
örneklerinden DNA izole edildikten sonra leptin, ghrelin ve IGF-1 gen
polimorfizmleri PCR-RFLP yöntemiyle belirlenmiştir. Sonuç olarak incelenen besi
sığırı sürüsünde IGF-1/SnaBI
polimorfizmi yönünden BB, AB ve AA genotiplerinin her üçü de gözlemlenmiştir.
IGF-1 lokusunda sıklığı en yüksek genotip AB, en düşük genotip ise AA şeklinde
bulunmuş olup B allelinin sıklığı (0.600) A alleline göre (0.400) yüksek olduğu
gözlenmiştir. Leptin/PstI polimorfizmi
yönünden bakıldığında da yine olası üç genotip gözlenmiştir. Bu lokusta sıklığı
en yüksek genotip CT, en düşük genotip ise TT olarak bulunmuş, C allelinin
sıklığı 0.571 bulunurken T allelinin sıklığı 0.429 olarak tespit edilmiştir.
Ghrelin/BfaI polimorfizmi yönünden
ise incelenen materyalde AA ve AG genotipleri gözlenirken GG genotipi
gözlenmemiştir. Buna bağlı olarak A allelinin sıklığı yüksek bulunurken
(0.938), G allel sıklığı ise oldukça düşük (0.062) bulunmuştur. Sonuç olarak
incelenen besi sığırı populasyonlarının IGF-1/SnaBI ve Ghrelin/BfaI
polimorfizmleri yönünden Hardy-Weinberg dengesinde, Leptin/PstI polimorfizmi yönünden ise dengede olmadığı belirlenmiştir.

Keywords

Genetik polimorfizm, Besi sığırları, Leptin, Ghrelin, IGF-1

Supporting Institution

Bingöl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

BAP-697-176-2014

Thanks

Bu çalışma Bingöl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir (Proje No: BAP-697-176-2014).

Determination of Genetic Polymorphisms of Leptin, Ghreline and Insulin Like Growth Factor-1 (IGF-1) Genes in Beef Cattle Raised in Turkey

Abstract

This
study was performed to determine polymorphisms in leptin, ghrelin and insulin
like growth factor-1 (IGF-1) genes and to identify genotype and alleles
frequencies in beef cattles raised in Sanliurfa province of Turkey. A total of
405 male beef cattles, which were raised in Harranova Live stock and
Agricultural Enterprise in Şanlıurfa and selected by phenotypic evaluation,
were included in to the study. The animal material consisted of Hereford
(n=112), Angus (n=145), Charolais (n=54), Black Hereford (n=36), Brahman
(n=24)  and Limousin (n=34) cattles.
After DNA isolation from meat samples, leptin, ghrelin and insulin like growth
factor-1 (IGF-1) gene polymorphisms were determined by using PCR-RFLP method.
All three possible genotypes of BB, AB and AA were observed in IGF-1/SnaBI gene
AB and AA genotypes had the highest and the lowest frequencies, respectively.
The frequency of B allele (0.600) was higher than that of A allele
(0.400).  With respect to PstI polymorphism
at the leptin gene, three possible genotypes were also observed. In this locus;
genotypes having the highest and the lowest frequency were CT and TT
respectively and the frequency of C allele was 0.571 while frequency of T was
0.429. At the BfaI polymorphic site of Ghrelin gene in AA and AG
genotypes were observed while GG genotype was not observed. Accordingly, the
frequency of the A allele was found to be high (0.938) and the frequency of the
G allele was found to be quite low (0.062). As a result, it was determined that
the examined beef cattle populations were in the Hardy-Weinberg equilibrium in
terms for IGF-1 / SnaBI and Ghrelin / BfaI polymorphisms but not
for Leptin/PstI polymorphism. 

Keywords

Genetic polymorphisms, Beef cattle, Leptin, Ghreline, IGF-1

Project Number

BAP-697-176-2014

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